LOCUS       sequence-266578-       10460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral backbone for inducible expression of human GBP1 missing 
            the reactive cysteine of the CaaX-box (GBP1-C589A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, 
            Frickel EM
  TITLE     Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis 
            and pyroptosis.
  JOURNAL   EMBO J. 2019 Jul 1;38(13):e100926. doi: 10.15252/embj.2018100926. 
            Epub 2019 Jun 3.
  PUBMED    31268602
REFERENCE   2  (bases 1 to 10460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2019-07-1"; volume: "38(13):e100926. doi"; pages: " 
            10.15252/embj.2018100926. Epub 2019 Jun 3"
COMMENT     Sequence Label: pLenti-Tet-GBP1-C589A
FEATURES             Location/Qualifiers
     source          1..10460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2649..2667
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2686..2704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2723..2741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2760..2778
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2797..2815
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2834..2852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2871..2889
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2908..2926
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2948..2972
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4937..5148
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5156..5165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5162..5536
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    5552..6140
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5605..5625)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6023..6034)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6212..6445
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6471..6488)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6477..6701
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6747..7175
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6834..6853)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7044..7065
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7184..7204)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7189..7518
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7369..7504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7431..7450
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7566..7613
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7632..8006
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8136..8257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8173..8192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8227..8246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8306..8322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8306..8322)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8319..8341)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8330..8346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8354..8360,8361..8378,8379..8384))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8399..8420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8537..8554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8708..9296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8788..8807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9467..10258,10259..10327))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10090..10109
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10328..10432)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccggg agtatccgct cgagtccgga tctcgacccg
     2641 ggcacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct atcagtgata
     2701 gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac gtctcgagct
     2761 ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat agagaagtcg
     2821 acacgtctcg agctccctat cagtgataga gaaggtacgt ctagaacgtc tccctatcag
     2881 tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga tagagaaggt accccctata
     2941 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     3001 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattt gctaggaatt
     3061 agcgctaccg gtgcggccgc accatggcat cagagatcca catgacaggc ccaatgtgcc
     3121 tcattgagaa cactaatggg cgactgatgg cgaatccaga agctctgaag atcctttctg
     3181 ccattacaca gcctatggtg gtggtggcaa ttgtgggcct ctaccgcaca ggcaaatcct
     3241 acctgatgaa caagctggct ggaaagaaaa agggcttctc tctgggctcc acggtgcagt
     3301 ctcacactaa aggaatctgg atgtggtgtg tgccccaccc caagaagcca ggccacatcc
     3361 tagttctgct ggacaccgag ggtctgggag atgtagagaa gggtgacaac cagaatgact
     3421 cctggatctt cgccctggcc gtcctcctga gcagcacctt cgtgtacaat agcataggaa
     3481 ccatcaacca gcaggctatg gaccaactgt actatgtgac agagctgaca catagaatcc
     3541 gatcaaaatc ctcacctgat gagaatgaga atgaggttga ggattcagct gactttgtga
     3601 gcttcttccc agactttgtg tggacactga gagatttctc cctggacttg gaagcagatg
     3661 gacaacccct cacaccagat gagtacctga catactccct gaagctgaag aaaggtacca
     3721 gtcaaaaaga tgaaactttt aacctgccca gactctgtat ccggaaattc ttcccaaaga
     3781 aaaaatgctt tgtctttgat cggcccgttc accgcaggaa gcttgcccag ctcgagaaac
     3841 tacaagatga agagctggac cccgaatttg tgcaacaagt agcagacttc tgttcctaca
     3901 tctttagtaa ttccaaaact aaaactcttt caggaggcat ccaggtcaac gggcctcgtc
     3961 tagagagcct ggtgctgacc tacgtcaatg ccatcagcag tggggatctg ccgtgcatgg
     4021 agaacgcagt cctggccttg gcccagatag agaactcagc tgcagtgcaa aaggctattg
     4081 cccactatga acagcagatg ggccagaagg tgcagctgcc cacagaaagc ctccaggagc
     4141 tgctggacct gcacagggac agtgagagag aggccattga agtcttcatc aggagttcct
     4201 tcaaagatgt ggaccatcta tttcaaaagg agttagcggc ccagctagaa aaaaagcggg
     4261 atgacttttg taaacagaat caggaagcat catcagatcg ttgctcagct ttacttcagg
     4321 tcattttcag tcctctagaa gaagaagtga aggcgggaat ttattcgaaa ccagggggct
     4381 atcgtctctt tgttcagaag ctacaagacc tgaagaaaaa gtactatgag gaaccgagga
     4441 aggggataca ggctgaagag attctgcaga catacttgaa atccaaggag tctatgactg
     4501 atgcaattct ccagacagac cagactctca cagaaaaaga aaaggagatt gaagtggaac
     4561 gtgtgaaagc tgagtctgca caggcttcag caaaaatgtt gcaggaaatg caaagaaaga
     4621 atgagcggat gatggaacag aaggagagga gttatcagga acacttgaaa caactgactg
     4681 agaagatgga gaacgacagg gtccagttgc tgaaagagca agagaggacc ctcgctctta
     4741 aacttcagga acaggagcaa ctactaaaag agggatttca aaaagaaagc agaataatga
     4801 aaaatgagat acaggatctc cagacgaaaa tgagacgacg aaaggcagct accataagct
     4861 aagatccctg atcgatcggc cgaattcgct agctaggtct tgaaaggagt gggaattggc
     4921 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     4981 ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     5041 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     5101 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagga ccggtgccac
     5161 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     5221 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     5281 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     5341 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     5401 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     5461 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     5521 cgaggagcag gactgaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     5581 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5641 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5701 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5761 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5821 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5881 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5941 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6001 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6061 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6121 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     6181 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6241 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     6301 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6361 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6421 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     6481 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6541 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6601 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6661 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6721 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6781 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6841 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6901 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6961 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7021 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7081 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7141 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     7201 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7261 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7321 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7381 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7441 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7501 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     7561 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     7621 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     7681 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     7741 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     7801 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     7861 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     7921 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     7981 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     8041 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     8101 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     8161 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8221 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     8281 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8341 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8401 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8461 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8521 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8581 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8641 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8701 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8761 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8821 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8881 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8941 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9001 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9061 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9121 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9181 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9241 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9301 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9361 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9421 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9481 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9541 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9601 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9661 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9721 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9781 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9841 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9901 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9961 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10021 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10081 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10141 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10201 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10261 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10321 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10381 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10441 cccgaaaagt gccacctgac
//
