LOCUS       sequence-266633-        5106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeted C-to-T in rice protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5106)
  TITLE     Gao Lab Plasmids
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: A3A-PBE-NG
FEATURES             Location/Qualifiers
     source          1..5106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4665..4673
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4753..4800
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4822..5070
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5083..5103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggaggcca gcccggctag cggcccaagg catctcatgg acccgcacat cttcaccagc
       61 aacttcaaca acggcatcgg caggcacaag acctacttgt gctacgaggt ggagaggctc
      121 gacaacggaa cctccgtgaa gatggaccaa cacagggggt tcctccacaa ccaagccaag
      181 aacctcctct gcggcttcta cggcaggcac gccgagttga ggttcctcga cttggtgcca
      241 tccctccaac tcgatccagc ccaaatctac cgcgtgacct ggttcatctc ctggtcccca
      301 tgcttctcct ggggttgcgc cggcgaggtt cgggctttcc tccaagaaaa cacccacgtc
      361 cgcctccgca ttttcgccgc caggatctat gattacgacc ctctctacaa ggaggccctc
      421 cagatgctgc gggacgccgg tgctcaggtg agtatcatga cctacgacga gttcaagcac
      481 tgctgggaca ccttcgttga ccaccagggc tgcccattcc aaccatggga cggtctggat
      541 gaacacagcc aagccttgtc cggcaggctc cgggccatcc tccaaaacca ggggaactcc
      601 gggagcgaga cgccaggcac ctccgagtcg gccaccccag aatctcttaa ggacaagaag
      661 tactcgatcg gcctcgccat tgggactaac tctgttggct gggccgtgat caccgacgag
      721 tacaaggtgc cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag
      781 aagaatctca ttggcgctct cctgttcgac agcggcgaga cggctgaggc tacgcggctc
      841 aagcgcaccg cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta cctgcaggag
      901 attttctcca acgagatggc gaaggttgac gattctttct tccacaggct ggaggagtca
      961 ttcctcgtgg aggaggataa gaagcacgag cggcatccaa tcttcggcaa cattgtcgac
     1021 gaggttgcct accacgagaa gtaccctacg atctaccatc tgcggaagaa gctcgtggac
     1081 tccacagata aggcggacct ccgcctgatc tacctcgctc tggcccacat gattaagttc
     1141 aggggccatt tcctgatcga gggggatctc aacccggaca atagcgatgt tgacaagctg
     1201 ttcatccagc tcgtgcagac gtacaaccag ctcttcgagg agaaccccat taatgcgtca
     1261 ggcgtcgacg cgaaggctat cctgtccgct aggctctcga agtctcggcg cctcgagaac
     1321 ctgatcgccc agctgccggg cgagaagaag aacggcctgt tcgggaatct cattgcgctc
     1381 agcctggggc tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga cgccaagctg
     1441 cagctctcca aggacacata cgacgatgac ctggataacc tcctggccca gatcggcgat
     1501 cagtacgcgg acctgttcct cgctgccaag aatctgtcgg acgccatcct cctgtctgat
     1561 attctcaggg tgaacaccga gattacgaag gctccgctct cagcctccat gatcaagcgc
     1621 tacgacgagc accatcagga tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc
     1681 gagaagtaca aggagatctt cttcgatcag tcgaagaacg gctacgctgg gtacattgac
     1741 ggcggggcct ctcaggagga gttctacaag ttcatcaagc cgattctgga gaagatggac
     1801 ggcacggagg agctgctggt gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca
     1861 ttcgataacg gcagcatccc acaccagatt catctcgggg agctgcacgc tatcctgagg
     1921 aggcaggagg acttctaccc tttcctcaag gataaccgcg agaagatcga gaagattctg
     1981 actttcagga tcccgtacta cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg
     2041 atgacccgca agtcagagga gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag
     2101 ggcgctagcg ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa cctgccaaat
     2161 gagaaggtgc tccctaagca ctcgctcctg tacgagtact tcacagtcta caacgagctg
     2221 actaaggtga agtatgtgac cgagggcatg aggaagccgg ctttcctgtc tggggagcag
     2281 aagaaggcca tcgtggacct cctgttcaag accaaccgga aggtcacggt taagcagctc
     2341 aaggaggact acttcaagaa gattgagtgc ttcgattcgg tcgagatctc tggcgttgag
     2401 gaccgcttca acgcctccct ggggacctac cacgatctcc tgaagatcat taaggataag
     2461 gacttcctgg acaacgagga gaatgaggat atcctcgagg acattgtgct gacactcact
     2521 ctgttcgagg accgggagat gatcgaggag cgcctgaaga cttacgccca tctcttcgat
     2581 gacaaggtca tgaagcagct caagaggagg aggtacaccg gctgggggag gctgagcagg
     2641 aagctcatca acggcattcg ggacaagcag tccgggaaga cgatcctcga cttcctgaag
     2701 agcgatggct tcgcgaaccg caatttcatg cagctgattc acgatgacag cctcacattc
     2761 aaggaggata tccagaaggc tcaggtgagc ggccaggggg actcgctgca cgagcatatc
     2821 gcgaacctcg ctggctcgcc agctatcaag aaggggattc tgcagaccgt gaaggttgtg
     2881 gacgagctgg tgaaggtcat gggcaggcac aagcctgaga acatcgtcat tgagatggcc
     2941 cgggagaatc agaccacgca gaagggccag aagaactcac gcgagaggat gaagaggatc
     3001 gaggagggca ttaaggagct ggggtcccag atcctcaagg agcacccggt ggagaacacg
     3061 cagctgcaga atgagaagct ctacctgtac tacctccaga atggccgcga tatgtatgtg
     3121 gaccaggagc tggatattaa caggctcagc gattacgacg tcgatcatat cgttccacag
     3181 tcattcctga aggatgactc cattgacaac aaggtcctca ccaggtcgga caagaaccgg
     3241 ggcaagtctg ataatgttcc ttcagaggag gtcgttaaga agatgaagaa ctactggcgc
     3301 cagctcctga atgccaagct gatcacgcag cggaagttcg ataacctcac aaaggctgag
     3361 aggggcgggc tctctgagct ggacaaggcg ggcttcatca agaggcagct ggtcgagaca
     3421 cggcagatca ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac taagtacgat
     3481 gagaatgaca agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc
     3541 gacttcagga aggatttcca gttctacaag gttcgggaga tcaacaatta ccaccatgcc
     3601 catgacgcgt acctgaacgc ggtggtcggc acagctctga tcaagaagta cccaaagctc
     3661 gagagcgagt tcgtgtacgg ggactacaag gtttacgatg tgaggaagat gatcgccaag
     3721 tcggagcagg agattggcaa ggctaccgcc aagtacttct tctactctaa cattatgaat
     3781 ttcttcaaga cagagatcac tctggccaat ggcgagatcc ggaagcgccc cctcatcgag
     3841 acgaacggcg agacggggga gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg
     3901 aaggttctct ccatgccaca agtgaatatc gtcaagaaga cagaggtcca gactggcggg
     3961 ttctctaagg agtcaattcg gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag
     4021 gactgggatc cgaagaagta cggcgggttc gtcagcccca ctgtggccta ctcggtcctg
     4081 gttgtggcga aggttgagaa gggcaagtcc aagaagctca agagcgtgaa ggagctgctg
     4141 gggatcacga ttatggagcg ctccagcttc gagaagaacc cgatcgattt cctggaggcg
     4201 aagggctaca aggaggtgaa gaaggacctg atcattaagc tccccaagta ctcactcttc
     4261 gagctggaga acggcaggaa gcggatgctg gcttccgctc gcttcctgca gaaggggaac
     4321 gagctggctc tgccgtccaa gtatgtgaac ttcctctacc tggcctccca ctacgagaag
     4381 ctcaagggca gccccgagga caacgagcag aagcagctgt tcgtcgagca gcacaagcat
     4441 tacctcgacg agatcattga gcagatttcc gagttctcca agcgcgtgat cctggccgac
     4501 gcgaatctgg ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc aatcagggag
     4561 caggctgaga atatcattca tctcttcacc ctgacgaacc tcggcgcccc tcgtgctttc
     4621 aagtacttcg acacaactat cgatcgcaag gtatacagaa gcactaagga ggtcctggac
     4681 gcgaccctca tccaccagtc gattaccggc ctctacgaga cgcgcatcga cctgtctcag
     4741 ctcgggggcg acaagcggcc agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag
     4801 acccgcgact ccggcggcag cacgaacctc tccgacatca tcgagaagga gacgggcaag
     4861 cagctcgtga tccaggagag catcctcatg ctgccggagg aggtggagga ggtcatcggc
     4921 aacaagcccg agtccgacat cctcgtgcac accgcctacg acgagtccac ggacgagaac
     4981 gtcatgctcc tgacgagcga cgctccagag tacaagccat gggctctcgt gatccaggac
     5041 agcaacggcg agaacaagat caagatgctg tccggcggct ccccaaagaa gaagcggaag
     5101 gtgtag
//
