LOCUS       sequence-266646-       12109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PspCas13b in mammalian cells, localized in the nucleus. 
            For cloning of guide RNAs compatible with PspCas13b. Contains a 3' 
            direct repeat. Clone using BsmBI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Wessels HH, Mendez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana 
            NE
  TITLE     Massively parallel Cas13 screens reveal principles for guide RNA 
            design.
  JOURNAL   Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 
            10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
  PUBMED    32518401
REFERENCE   2  (bases 1 to 12109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0456-9"; journalName: "Nat Biotechnol"; date: 
            "2020-06"; volume: "38"; issue: "6"; pages: "722-727"
COMMENT     Sequence Label: pLentiRNACRISPR_003 -
            hU6-DR_BsmBI-EFS-PspCas1...
FEATURES             Location/Qualifiers
     source          1..12109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2608..2856
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2608..2628
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2779..2798
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2969..3180
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6475..6522
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6532..6588
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6589..7185
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7082..7102
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7201..7789
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7254..7274)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7672..7683)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7861..8094
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8120..8137)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8126..8350
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8396..8824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8483..8502)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8693..8714
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8833..8853)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8838..9167
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9018..9153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9080..9099
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9215..9262
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9281..9655
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9785..9906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9822..9841)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9876..9895
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9955..9971)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9955..9971)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9968..9990)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9979..9995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10003..10009,10010..10027,10028..10033))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10048..10069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10186..10203)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10357..10945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10437..10456)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11116..11907,11908..11976))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11739..11758
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11977..12081)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccgaga cggacgtctc tgttgtggaa
     2881 ggtccagttt tgaggggcta ttacaacttt tttgaattcg ctagctaggt cttgaaagga
     2941 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     3001 aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac
     3061 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     3121 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     3181 gaccggttct agagcgctgc caccatgaac atccccgctc tggtggaaaa ccagaagaag
     3241 tactttggca cctacagcgt gatggccatg ctgaacgctc agaccgtgct ggaccacatc
     3301 cagaaggtgg ccgatattga gggcgagcag aacgagaaca acgagaatct gtggtttcac
     3361 cccgtgatga gccacctgta caacgccaag aacggctacg acaagcagcc cgagaaaacc
     3421 atgttcatca tcgagcggct gcagagctac ttcccattcc tgaagatcat ggccgagaac
     3481 cagagagagt acagcaacgg caagtacaag cagaaccgcg tggaagtgaa cagcaacgac
     3541 atcttcgagg tgctgaagcg cgccttcggc gtgctgaaga tgtacaggga cctgaccaac
     3601 cactacaaga cctacgagga aaagctgaac gacggctgcg agttcctgac cagcacagag
     3661 caacctctga gcggcatgat caacaactac tacacagtgg ccctgcggaa catgaacgag
     3721 agatacggct acaagacaga ggacctggcc ttcatccagg acaagcggtt caagttcgtg
     3781 aaggacgcct acggcaagaa aaagtcccaa gtgaataccg gattcttcct gagcctgcag
     3841 gactacaacg gcgacacaca gaagaagctg cacctgagcg gagtgggaat cgccctgctg
     3901 atctgcctgt tcctggacaa gcagtacatc aacatctttc tgagcaggct gcccatcttc
     3961 tccagctaca atgcccagag cgaggaacgg cggatcatca tcagatcctt cggcatcaac
     4021 agcatcaagc tgcccaagga ccggatccac agcgagaagt ccaacaagag cgtggccatg
     4081 gatatgctca acgaagtgaa gcggtgcccc gacgagctgt tcacaacact gtctgccgag
     4141 aagcagtccc ggttcagaat catcagcgac gaccacaatg aagtgctgat gaagcggagc
     4201 agcgacagat tcgtgcctct gctgctgcag tatatcgatt acggcaagct gttcgaccac
     4261 atcaggttcc acgtgaacat gggcaagctg agatacctgc tgaaggccga caagacctgc
     4321 atcgacggcc agaccagagt cagagtgatc gagcagcccc tgaacggctt cggcagactg
     4381 gaagaggccg agacaatgcg gaagcaagag aacggcacct tcggcaacag cggcatccgg
     4441 atcagagact tcgagaacat gaagcgggac gacgccaatc ctgccaacta tccctacatc
     4501 gtggacacct acacacacta catcctggaa aacaacaagg tcgagatgtt tatcaacgac
     4561 aaagaggaca gcgccccact gctgcccgtg atcgaggatg atagatacgt ggtcaagaca
     4621 atccccagct gccggatgag caccctggaa attccagcca tggccttcca catgtttctg
     4681 ttcggcagca agaaaaccga gaagctgatc gtggacgtgc acaaccggta caagagactg
     4741 ttccaggcca tgcagaaaga agaagtgacc gccgagaata tcgccagctt cggaatcgcc
     4801 gagagcgacc tgcctcagaa gatcctggat ctgatcagcg gcaatgccca cggcaaggat
     4861 gtggacgcct tcatcagact gaccgtggac gacatgctga ccgacaccga gcggagaatc
     4921 aagagattca aggacgaccg gaagtccatt cggagcgccg acaacaagat gggaaagaga
     4981 ggcttcaagc agatctccac aggcaagctg gccgacttcc tggccaagga catcgtgctg
     5041 tttcagccca gcgtgaacga tggcgagaac aagatcaccg gcctgaacta ccggatcatg
     5101 cagagcgcca ttgccgtgta cgatagcggc gacgattacg aggccaagca gcagttcaag
     5161 ctgatgttcg agaaggcccg gctgatcggc aagggcacaa cagagcctca tccatttctg
     5221 tacaaggtgt tcgcccgcag catccccgcc aatgccgtcg agttctacga gcgctacctg
     5281 atcgagcgga agttctacct gaccggcctg tccaacgaga tcaagaaagg caacagagtg
     5341 gatgtgccct tcatccggcg ggaccagaac aagtggaaaa cacccgccat gaagaccctg
     5401 ggcagaatct acagcgagga tctgcccgtg gaactgccca gacagatgtt cgacaatgag
     5461 atcaagtccc acctgaagtc cctgccacag atggaaggca tcgacttcaa caatgccaac
     5521 gtgacctatc tgatcgccga gtacatgaag agagtgctgg acgacgactt ccagaccttc
     5581 taccagtgga accgcaacta ccggtacatg gacatgctta agggcgagta cgacagaaag
     5641 ggctccctgc agcactgctt caccagcgtg gaagagagag aaggcctctg gaaagagcgg
     5701 gcctccagaa cagagcggta cagaaagcag gccagcaaca agatccgcag caaccggcag
     5761 atgagaaacg ccagcagcga agagatcgag acaatcctgg ataagcggct gagcaacagc
     5821 cggaacgagt accagaaaag cgagaaagtg atccggcgct acagagtgca ggatgccctg
     5881 ctgtttctgc tggccaaaaa gaccctgacc gaactggccg atttcgacgg cgagaggttc
     5941 aaactgaaag aaatcatgcc cgacgccgag aagggaatcc tgagcgagat catgcccatg
     6001 agcttcacct tcgagaaagg cggcaagaag tacaccatca ccagcgaggg catgaagctg
     6061 aagaactacg gcgacttctt tgtgctggct agcgacaaga ggatcggcaa cctgctggaa
     6121 ctcgtgggca gcgacatcgt gtccaaagag gatatcatgg aagagttcaa caaatacgac
     6181 cagtgcaggc ccgagatcag ctccatcgtg ttcaacctgg aaaagtgggc cttcgacaca
     6241 taccccgagc tgtctgccag agtggaccgg gaagagaagg tggacttcaa gagcatcctg
     6301 aaaatcctgc tgaacaacaa gaacatcaac aaagagcaga gcgacatcct gcggaagatc
     6361 cggaacgcct tcgatcacaa caattacccc gacaaaggcg tggtggaaat caaggccctg
     6421 cctgagatcg ccatgagcat caagaaggcc tttggggagt acgccatcat gaagaagcga
     6481 cctgccgcca caaagaaggc tggacaggct aagaagaaga aaggatccgg cgcaacaaac
     6541 ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgac cgagtacaag
     6601 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6661 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6721 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6781 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6841 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6901 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6961 gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7021 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7081 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7141 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaacgcg ttaagtcgac
     7201 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7261 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7321 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7381 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7441 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7501 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7561 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     7621 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7681 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7741 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     7801 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7861 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7921 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7981 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8041 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     8101 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     8161 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     8221 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     8281 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     8341 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     8401 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8461 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8521 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8581 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8641 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8701 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8761 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8821 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     8881 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8941 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     9001 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     9061 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     9121 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     9181 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     9241 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     9301 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     9361 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     9421 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     9481 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     9541 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     9601 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     9661 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     9721 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     9781 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9841 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9901 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9961 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    10021 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    10081 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    10141 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10201 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10261 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10321 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10381 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10441 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10501 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10561 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10621 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10681 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10741 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10801 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10861 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10921 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10981 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11041 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11101 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11161 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11221 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11281 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11341 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11401 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11461 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11521 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11581 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11641 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11701 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    11761 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11821 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11881 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11941 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12001 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12061 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
