LOCUS       sequence-266648-       12103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PspCas13b in mammalian cells, localized in the cytoplasm. 
            For cloning guide RNAs compatible with PspCas13b. Contains a 3' 
            direct repeat. Clone using BsmBI. F overhang acac. R overhang caac.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12103)
  AUTHORS   Wessels HH, Mendez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana 
            NE
  TITLE     Massively parallel Cas13 screens reveal principles for guide RNA 
            design.
  JOURNAL   Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 
            10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
  PUBMED    32518401
REFERENCE   2  (bases 1 to 12103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0456-9"; journalName: "Nat Biotechnol"; date: 
            "2020-06"; volume: "38"; issue: "6"; pages: "722-727"
COMMENT     Sequence Label: pLentiRNACRISPR_004 -
            hU6-DR_BsmBI-EFS-PspCas1...
FEATURES             Location/Qualifiers
     source          1..12103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2608..2856
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2608..2628
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2779..2798
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2969..3180
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6481..6507
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             6526..6582
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6583..7179
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7076..7096
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7195..7783
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7248..7268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7666..7677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7855..8088
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8114..8131)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8120..8344
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8390..8818
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8477..8496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8687..8708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8827..8847)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8832..9161
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9012..9147
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9074..9093
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9209..9256
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9275..9649
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9779..9900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9816..9835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9870..9889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9949..9965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9949..9965)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9962..9984)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9973..9989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9997..10003,10004..10021,10022..10027))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10042..10063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10180..10197)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10351..10939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10431..10450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11110..11901,11902..11970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11733..11752
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11971..12075)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccgaga cggacgtctc tgttgtggaa
     2881 ggtccagttt tgaggggcta ttacaacttt tttgaattcg ctagctaggt cttgaaagga
     2941 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     3001 aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac
     3061 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     3121 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     3181 gaccggttct agagcgctgc caccatgaac atccccgctc tggtggaaaa ccagaagaag
     3241 tactttggca cctacagcgt gatggccatg ctgaacgctc agaccgtgct ggaccacatc
     3301 cagaaggtgg ccgatattga gggcgagcag aacgagaaca acgagaatct gtggtttcac
     3361 cccgtgatga gccacctgta caacgccaag aacggctacg acaagcagcc cgagaaaacc
     3421 atgttcatca tcgagcggct gcagagctac ttcccattcc tgaagatcat ggccgagaac
     3481 cagagagagt acagcaacgg caagtacaag cagaaccgcg tggaagtgaa cagcaacgac
     3541 atcttcgagg tgctgaagcg cgccttcggc gtgctgaaga tgtacaggga cctgaccaac
     3601 cactacaaga cctacgagga aaagctgaac gacggctgcg agttcctgac cagcacagag
     3661 caacctctga gcggcatgat caacaactac tacacagtgg ccctgcggaa catgaacgag
     3721 agatacggct acaagacaga ggacctggcc ttcatccagg acaagcggtt caagttcgtg
     3781 aaggacgcct acggcaagaa aaagtcccaa gtgaataccg gattcttcct gagcctgcag
     3841 gactacaacg gcgacacaca gaagaagctg cacctgagcg gagtgggaat cgccctgctg
     3901 atctgcctgt tcctggacaa gcagtacatc aacatctttc tgagcaggct gcccatcttc
     3961 tccagctaca atgcccagag cgaggaacgg cggatcatca tcagatcctt cggcatcaac
     4021 agcatcaagc tgcccaagga ccggatccac agcgagaagt ccaacaagag cgtggccatg
     4081 gatatgctca acgaagtgaa gcggtgcccc gacgagctgt tcacaacact gtctgccgag
     4141 aagcagtccc ggttcagaat catcagcgac gaccacaatg aagtgctgat gaagcggagc
     4201 agcgacagat tcgtgcctct gctgctgcag tatatcgatt acggcaagct gttcgaccac
     4261 atcaggttcc acgtgaacat gggcaagctg agatacctgc tgaaggccga caagacctgc
     4321 atcgacggcc agaccagagt cagagtgatc gagcagcccc tgaacggctt cggcagactg
     4381 gaagaggccg agacaatgcg gaagcaagag aacggcacct tcggcaacag cggcatccgg
     4441 atcagagact tcgagaacat gaagcgggac gacgccaatc ctgccaacta tccctacatc
     4501 gtggacacct acacacacta catcctggaa aacaacaagg tcgagatgtt tatcaacgac
     4561 aaagaggaca gcgccccact gctgcccgtg atcgaggatg atagatacgt ggtcaagaca
     4621 atccccagct gccggatgag caccctggaa attccagcca tggccttcca catgtttctg
     4681 ttcggcagca agaaaaccga gaagctgatc gtggacgtgc acaaccggta caagagactg
     4741 ttccaggcca tgcagaaaga agaagtgacc gccgagaata tcgccagctt cggaatcgcc
     4801 gagagcgacc tgcctcagaa gatcctggat ctgatcagcg gcaatgccca cggcaaggat
     4861 gtggacgcct tcatcagact gaccgtggac gacatgctga ccgacaccga gcggagaatc
     4921 aagagattca aggacgaccg gaagtccatt cggagcgccg acaacaagat gggaaagaga
     4981 ggcttcaagc agatctccac aggcaagctg gccgacttcc tggccaagga catcgtgctg
     5041 tttcagccca gcgtgaacga tggcgagaac aagatcaccg gcctgaacta ccggatcatg
     5101 cagagcgcca ttgccgtgta cgatagcggc gacgattacg aggccaagca gcagttcaag
     5161 ctgatgttcg agaaggcccg gctgatcggc aagggcacaa cagagcctca tccatttctg
     5221 tacaaggtgt tcgcccgcag catccccgcc aatgccgtcg agttctacga gcgctacctg
     5281 atcgagcgga agttctacct gaccggcctg tccaacgaga tcaagaaagg caacagagtg
     5341 gatgtgccct tcatccggcg ggaccagaac aagtggaaaa cacccgccat gaagaccctg
     5401 ggcagaatct acagcgagga tctgcccgtg gaactgccca gacagatgtt cgacaatgag
     5461 atcaagtccc acctgaagtc cctgccacag atggaaggca tcgacttcaa caatgccaac
     5521 gtgacctatc tgatcgccga gtacatgaag agagtgctgg acgacgactt ccagaccttc
     5581 taccagtgga accgcaacta ccggtacatg gacatgctta agggcgagta cgacagaaag
     5641 ggctccctgc agcactgctt caccagcgtg gaagagagag aaggcctctg gaaagagcgg
     5701 gcctccagaa cagagcggta cagaaagcag gccagcaaca agatccgcag caaccggcag
     5761 atgagaaacg ccagcagcga agagatcgag acaatcctgg ataagcggct gagcaacagc
     5821 cggaacgagt accagaaaag cgagaaagtg atccggcgct acagagtgca ggatgccctg
     5881 ctgtttctgc tggccaaaaa gaccctgacc gaactggccg atttcgacgg cgagaggttc
     5941 aaactgaaag aaatcatgcc cgacgccgag aagggaatcc tgagcgagat catgcccatg
     6001 agcttcacct tcgagaaagg cggcaagaag tacaccatca ccagcgaggg catgaagctg
     6061 aagaactacg gcgacttctt tgtgctggct agcgacaaga ggatcggcaa cctgctggaa
     6121 ctcgtgggca gcgacatcgt gtccaaagag gatatcatgg aagagttcaa caaatacgac
     6181 cagtgcaggc ccgagatcag ctccatcgtg ttcaacctgg aaaagtgggc cttcgacaca
     6241 taccccgagc tgtctgccag agtggaccgg gaagagaagg tggacttcaa gagcatcctg
     6301 aaaatcctgc tgaacaacaa gaacatcaac aaagagcaga gcgacatcct gcggaagatc
     6361 cggaacgcct tcgatcacaa caattacccc gacaaaggcg tggtggaaat caaggccctg
     6421 cctgagatcg ccatgagcat caagaaggcc tttggggagt acgccatcat gaagcttcaa
     6481 ctgcctccac ttgaaagact gacactgggt agttccggat ccggcgcaac aaacttctct
     6541 ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgaccgagta caagcccacg
     6601 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6661 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6721 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6781 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6841 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6901 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6961 gtcggagtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     7021 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     7081 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     7141 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttaagt cgacaatcaa
     7201 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7261 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7321 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7381 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7441 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7501 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7561 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7621 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7681 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7741 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     7801 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7861 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7921 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7981 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8041 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     8101 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     8161 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     8221 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     8281 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     8341 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     8401 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8461 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8521 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8581 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8641 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8701 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8761 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     8821 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8881 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     8941 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     9001 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9061 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     9121 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     9181 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     9241 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     9301 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     9361 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     9421 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     9481 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     9541 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     9601 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     9661 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     9721 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     9781 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9841 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9901 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     9961 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10021 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10081 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10141 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10201 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10261 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11101 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11161 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11221 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11281 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11341 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11401 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11461 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11521 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11581 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11641 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11701 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11761 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11821 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11881 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11941 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    12001 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    12061 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
