LOCUS       sequence-266661-        5262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of C-terminally FLAG-tagged human RPL26 mutant lacking 
            the C-terminal UFMylation sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Wang L, Xu Y, Rogers H, Saidi L, Noguchi CT, Li H, Yewdell JW, 
            Guydosh NR, Ye Y
  TITLE     UFMylation of RPL26 links translocation-associated quality control 
            to endoplasmic reticulum protein homeostasis.
  JOURNAL   Cell Res. 2020 Jan;30(1):5-20. doi: 10.1038/s41422-019-0236-6. Epub 
            2019 Oct 8.
  PUBMED    31595041
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-019-0236-6"; journalName: "Cell Res"; date: 
            "2020-01"; volume: "30"; issue: "1"; pages: "5-20"
COMMENT     Sequence Label: pCMV6- Entry-RPL26DC-DDK
FEATURES             Location/Qualifiers
     source          1..5262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      67..522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(154..173)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     364..385
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     648..670
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     662..679
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     663..679
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        840..1219
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1220..1423
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1374..1394
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1449..1468
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1449..1467
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1925..1954
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1973..1996
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2016..2032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2016..2032)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2043..2665
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2242..2261)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(2814..3402)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2894..2913)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3731..3778
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3803..3822
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4010..4804)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4010..4804)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4121..4140)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4731..4750
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4839..5196)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4853..4988
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        5005..5196
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5153..5173
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(join(5198..5262,1..40))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
       61 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      121 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      181 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      241 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      301 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      361 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      421 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      481 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      541 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      601 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      661 ttgtaaaacg acggccagtg ccaagctgat ctatacattg aatcaatatt ggcaattagc
      721 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
      781 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
      841 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      901 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      961 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1021 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1081 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1141 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
     1201 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
     1261 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1321 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
     1381 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     1441 gaattttgta atacgactca ctatagggcg gccgggaatt cgtcgactgg atccggtacc
     1501 gaggagatct gccgccgcga tcgccatgaa gtttaatccc tttgtgactt ccgaccgaag
     1561 caagaatcgc aaaaggcatt tcaatgcacc ttcccacatt cgaaggaaga ttatgtcttc
     1621 ccctctttcc aaagagctga gacagaagta caacgtgcga tccatgccca tccgaaagga
     1681 tgatgaagtt caggttgtac gtggacacta taaaggtcag caaattggca aagtagtcca
     1741 ggtttacagg aagaaatatg ttatctacat tgaacgggtg cagcgggaaa aggctaatgg
     1801 cacaactgtc cacgtaggca ttcaccccag caaggtggtt atcactaggc taaaactgga
     1861 caaagaccgc aaaaagatcc tcgaacggaa agccaaatct cgccaaacgc gtacgcggcc
     1921 gctcgagcag aaactcatct cagaagagga tctggcagca aatgatatcc tggattacaa
     1981 ggatgacgac gataaggttt aaacggccgg ccgcggtcat agctgtttcc tgaacagatc
     2041 ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
     2101 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
     2161 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
     2221 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
     2281 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
     2341 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
     2401 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
     2461 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
     2521 tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc
     2581 atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt
     2641 ccactcagta gatgcctgtt gaattgggta cgcggccagc ggcgagcggt atcagctcac
     2701 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2761 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2821 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2881 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2941 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3001 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3061 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3121 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3181 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3241 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3301 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3361 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3421 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3481 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3541 taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg
     3601 agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg
     3661 tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc
     3721 ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc
     3781 atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg
     3841 tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg
     3901 aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat
     3961 ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact
     4021 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     4081 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     4141 ctatgtcctg atagcgatcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     4201 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     4261 cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     4321 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     4381 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     4441 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     4501 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     4561 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     4621 cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     4681 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     4741 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     4801 tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc taggcctcca
     4861 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     4921 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     4981 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     5041 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     5101 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     5161 tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt caatattatt
     5221 gaagcattta tcagggttat tgtctcatga gcggatacat at
//
