LOCUS       sequence-266687-        3288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Positive selection vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Jeske M, Altenbuchner J
  TITLE     The Escherichia coli rhamnose promoter rhaP(BAD) is in Pseudomonas 
            putida KT2440 independent of Crp-cAMP activation.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Feb;85(6):1923-33. doi: 
            10.1007/s00253-009-2245-8. Epub 2009 Sep 30.
  PUBMED    19789867
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-009-2245-8"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-02"; volume: "85"; issue: "6"; pages: 
            "1923-33"
COMMENT     Sequence Label: pJOE4786.1
FEATURES             Location/Qualifiers
     source          1..3288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..52)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     152..171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     445..464
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        445..463
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     699..721
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     713..730
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     714..730
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(746..762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(746..762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(759..781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    770..786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(794..800,801..818,819..824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    839..860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        complement(1071..1089)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1072..1089)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1299..1316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1470..2058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1550..1569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2229..3020,3021..3089))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2852..2871
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3090..3194)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3262..3280
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
      241 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      301 ttacgccaat ctagatccgg tggatgacct tttgaatgac ctttaataga ttatattact
      361 aattaattgg ggaccctaga ggtccccttt tttattttaa aaattttttc acaaaacggt
      421 ttacaagcgt aaagcttgca tgcctaatac gactcactat agggaaatct agaggatccc
      481 cgggtaccat gggcccgcgg ccgcctcgag aattcatcga tacgtagccg gctagcgata
      541 tcagatctac gcgtacgagc tcgaattcat cgatcattcg ccattcaggc tgcgcaactg
      601 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      661 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      721 gacggccagt gaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      781 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
      841 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
      901 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      961 gatcgatgaa ttcgagctcg tacgcgtaga tctgatatcg ctagccggct acgtatcgat
     1021 gaattctcga ggcggccgcg ggcccatggt acccggggat cctctagatt tctatagtgt
     1081 cacctaaatc gtatgcatgc aagctttacg cttgtaaacc gttttgtgaa aaaattttta
     1141 aaataaaaaa ggggacctct agggtcccca attaattagt aatataatct attaaaggtc
     1201 attcaaaagg tcatccaccg gatctagatc gatctgcatt aatgaatcgg ccaacgcgcg
     1261 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1321 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1381 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1441 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1501 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1561 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1621 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1681 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1741 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1801 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1861 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1921 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1981 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2041 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2101 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2161 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2221 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2281 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2341 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2401 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2461 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2521 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2581 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2641 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2701 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2761 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2821 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2881 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2941 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3001 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3061 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3121 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3181 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3241 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgt
//
