LOCUS       sequence-26669-e         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Enslen H, Raingeaud J, Davis RJ
  TITLE     Selective activation of p38 mitogen-activated protein (MAP) kinase 
            isoforms by the MAP kinase kinases MKK3 and MKK6.
  JOURNAL   J Biol Chem. 1998 Jan 16. 273(3):1741-8.
  PUBMED    9430721
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Jan 16. 273(3):1741-8."
COMMENT     Sequence Label: pCDNA3 Flag p38 gamma (agf)
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatcccg gcagccaaca tggactataa
      121 ggacgatgac gacaaaagct ccccgccacc cgcccgcaag ggcttttacc gccaggaggt
      181 gaccaaaacg gcctgggagg tgcgcgccgt gtaccaggac ctgcagcccg ttggctctgg
      241 tgcctatggt gcagtgtgct ctgcagtaga cagccgcact ggcaacaagg tggccatcaa
      301 gaagttgtac cggcccttcc agtcggagct gtttgccaag cgcgcctaca gagagttgcg
      361 cctcctcaaa cacatgcgcc acgagaacgt cattgggctg ctggatgtgt tcactcccga
      421 tgagactctg gatgacttca cagacttcta cctggtgatg ccattcatgg gcactgacct
      481 gggcaagctc atgaagcacg agactctgag tgaagacaga atccagtttc ttgtgtatca
      541 gatgctgaag gggctgaagt atatccacgc tgccggcgtc atccacaggg acttgaaacc
      601 tggaaacctg gctgtgaacg aggactgtga nctnaa
//
