LOCUS       sequence-266708-       10044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Gutscher M, Sobotta MC, Wabnitz GH, Ballikaya S, Meyer AJ, Samstag 
            Y, Dick TP
  TITLE     Proximity-based protein thiol oxidation by H2O2-scavenging 
            peroxidases.
  JOURNAL   J Biol Chem. 2009 Nov 13;284(46):31532-40. doi: 
            10.1074/jbc.M109.059246. Epub 2009 Sep 15.
  PUBMED    19755417
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.059246"; journalName: "J Biol Chem"; date: 
            "2009-11-13- 13"; volume: "284"; issue: "46"; pages: "31532-40"
COMMENT     Sequence Label: pEIGW roGFP2-Orp1
FEATURES             Location/Qualifiers
     source          1..10044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1304..1537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1722..1766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2033..2150
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2207..2418
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2465..2474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2471..2473,2474..2476,2477..2479,2480..3190)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MAVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGI
                     KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYK"
     primer_bind     complement(2519..2540)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2780..2799)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3127..3148
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3845..3861)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3846..3862)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    3875..4463
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3928..3948)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4346..4357)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4607..4640)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4660..4840
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4865..4883)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4866..4883)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5173..5192)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5406..5425)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5525..5547
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5585..5603)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5671..5775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5776..5844,5845..6636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5994..6013)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6807..7395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7296..7315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7549..7566
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7636..7656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7641..7970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7821..7956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9104..9169
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9299..9319
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9744..9878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9781..9800)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9835..9854
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     2041 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2101 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg
     2161 agactagcct cgagaagctt gatcgatggc tccggtgccc gtcagtgggc agagcgcaca
     2221 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     2281 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     2341 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     2401 tttgccgcca gaacacaggt gtcgtgacgc ggatcgggat cggcctttac ggccggttac
     2461 ccgggccacc atggcagtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2521 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2581 cgatgccacc tacggcaagc tgaccctgaa gttcatctcc accaccggca agctgcccgt
     2641 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     2701 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2761 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2821 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2881 catcctgggg cacaagctgg agtacaacta caactgccac aacgtctata tcatggccga
     2941 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     3001 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3061 gcccgacaac cactacctga gcacctgctc cgccctgagc aaagacccca acgagaagcg
     3121 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     3181 gctgtacaag actagtggtg gttcaggtgg tggtggttca ggtggtggtg gttcaggtgg
     3241 aggaggatca ggaggaggag gatcaggagg aggaggatca ggaggagaat tcgatatctc
     3301 agaattctat aagctagcac ctgttgacaa gaaaggccaa ccattcccct tcgaccaatt
     3361 aaagggaaaa gtggtgctta tcgttaatgt tgcctccaaa tgtggattca ctcctcaata
     3421 caaagaacta gaggccttgt acaaacgtta taaggacgaa ggatttacca tcatcgggtt
     3481 cccatgcaac cagtttggcc accaagaacc tggctctgat gaagaaattg cccagttctg
     3541 ccaactgaac tatggcgtga ctttccccat tatgaaaaaa attgacgtta atggtggcaa
     3601 tgaggaccct gtttacaagt ttttgaagag ccaaaaatcc ggtatgttgg gcttgagagg
     3661 tatcaaatgg aattttgaaa aattcttagt cgataaaaag ggtaaagtgt acgaaagata
     3721 ctcttcacta accaaacctt cttcgttgtc cgaaaccatc gaagaacttt tgaaagaggt
     3781 ggaataggcg gccgcgactc tagctagtcg agctcaagct tcgaattcga tatcaagctt
     3841 atcgcgatac cgtcgacctc gagggaattc cgataatcaa cctctggatt acaaaatttg
     3901 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3961 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4021 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4081 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4141 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4201 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4261 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     4321 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4381 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4441 ctccctttgg gccgcctccc cgcatcggga attcgagctc ggtaccttta agaccaatga
     4501 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4561 taattcactc ccaacgaaga caagatggga tcaattcacc atgggaataa cttcgtatag
     4621 catacattat acgaagttat gctgcttttt gcttgtactg ggtctctctg gttagaccag
     4681 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     4741 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     4801 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     4861 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     4921 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     4981 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     5041 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     5101 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     5161 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     5221 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     5281 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     5341 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     5401 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     5461 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5521 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     5581 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5641 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5701 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5761 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5821 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5881 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5941 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6001 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6061 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6121 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6181 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6241 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6301 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6361 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6421 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6481 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6541 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6601 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6661 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6721 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6781 cccgtagaaa agatcaaagg atattcttga gatccttttt ttctgcgcgt aatctgctgc
     6841 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6901 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6961 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7021 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7081 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7141 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7201 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7261 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7321 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7381 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7441 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7501 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7561 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7621 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7921 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
     7981 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     8041 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     8101 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     8161 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     8221 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     8281 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
     8341 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
     8401 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
     8461 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
     8521 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
     8581 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
     8641 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
     8701 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
     8761 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
     8821 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
     8881 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
     8941 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
     9001 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
     9061 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     9121 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     9181 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     9241 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     9301 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     9361 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     9421 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     9481 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     9541 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     9601 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     9661 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     9721 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     9781 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9841 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     9901 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     9961 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    10021 aaatatgtac tacaaactta gtag
//
