LOCUS       sequence-26672-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Enslen H, Raingeaud J, Davis RJ
  TITLE     Selective activation of p38 mitogen-activated protein (MAP) kinase 
            isoforms by the MAP kinase kinases MKK3 and MKK6.
  JOURNAL   J Biol Chem. 1998 Jan 16. 273(3):1741-8.
  PUBMED    9430721
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Jan 16. 273(3):1741-8."
COMMENT     Sequence Label: pCDNA3 Flag p38 beta2 (agf)
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aanccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatctaa gatggactac aaggacgatg
      121 acgataagtc gggccctcgc gccggcttct accggcagga gctgaacaag accgtgtggg
      181 aggtgccgct gcggctgcag gggctgcgcc cggtgggctc cggcgcctac ggctccgtct
      241 gttcggccta cgacgcccgg ctgcgccaga aggtggcggt gaagaagctg tcgcgcccct
      301 tccagtcgct gatccacgcg cgcagaacgt accgggagct gcggctgctc aagcacctga
      361 agcacgagaa cgtcatcggg cttctggacg tcttcacgcc ggccacgtcc atcgaggact
      421 tcagcgaagt gtacttggta accaccctga tgggcgccga cctgaacaac atcgtcaagt
      481 gccaggcgct gagcgacgag cacgttcaat tcctggttta ccagctgctg cgcgggctga
      541 agtacatcca ctcggccggg atcatccacc gggacctgaa gcccagcaac gtggctgtga
      601 acgaggactg tgagctcagg atcctggatt tcgggctggc gcgccaggcg gacgangaga
      661 tggccggctt tgtggccacg cgctggtacc gggcacctga gatcatgctc aactggttgc
      721 attacaacca aanagtggnn atctggtccg taggctg
//
