LOCUS       sequence-266732-        3414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluoride riboswitch-regulated catechol 2,3-dioxygenase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Thavarajah W, Silverman AD, Verosloff M, Kelley-Loughnane N, Jewett 
            MC, Lucks JB
  TITLE     Point-of-Use Detection of Environmental Fluoride via a Cell-Free 
            Riboswitch-Based Biosensor.
  JOURNAL   ACS Synth Biol. 2019 Dec 12. doi: 10.1021/acssynbio.9b00347.
  PUBMED    31829623
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Dec 12. doi: 10.1021/acssynbio.9b00347."
COMMENT     Sequence Label: pJBL7025
FEATURES             Location/Qualifiers
     source          1..3414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(289..306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(423..968)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(503..522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1162..1196
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     terminator      2252..2323
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2339..2366
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2522..3181
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2588..2607)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3202..3296
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 cggcgcaaag tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt
       61 tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg
      121 gtggtgcgta acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt
      181 actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc
      241 tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga
      301 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga
      361 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
      421 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
      481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
      541 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
      601 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
      661 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      721 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
      781 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
      841 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
      901 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
      961 gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta
     1021 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt
     1081 tgacagctta tcatcgataa gcttccgatg gcgcgccgag aggctttaca ctttatgctt
     1141 ccggctgaat tctaaagatc tttgacagct agctcagtcc taggtataat actagtttat
     1201 aggcgatgga gttcgccata aacgctgctt agctaatgac tcctaccagt atcactactg
     1261 gtaggagtct atttttttag gaggaaggat ctatgaacaa aggtgtaatg cgaccgggcc
     1321 atgtgcagct gcgtgtactg gacatgagca aggccctgga acactacgtc gagttgctgg
     1381 gcctgatcga gatggaccgt gacgaccagg gccgtgtcta tctgaaggct tggaccgaag
     1441 tggataagtt ttccctggtg ctacgcgagg ctgacgagcc gggcatggat tttatgggtt
     1501 tcaaggttgt ggatgaggat gctctccggc aactggagcg ggatctgatg gcatatggct
     1561 gtgccgttga gcagctaccc gcaggtgaac tgaacagttg tggccggcgc gtgcgcttcc
     1621 aggccccctc cgggcatcac ttcgagttgt atgcagacaa ggaatatact ggaaagtggg
     1681 gtttgaatga cgtcaatccc gaggcatggc cgcgcgatct gaaaggtatg gcggctgtgc
     1741 gtttcgacca cgccctcatg tatggcgacg aattgccggc gacctatgac ctgttcacca
     1801 aggtgctcgg tttctatctg gccgaacagg tgctggacga aaatggcacg cgcgtcgccc
     1861 agtttctcag tctgtcgacc aaggcccacg acgtggcctt cattcaccat ccggaaaaag
     1921 gccgcctcca tcatgtgtcc ttccacctcg aaacctggga agacttgctt cgcgccgccg
     1981 acctgatctc catgaccgac acatctatcg atatcggccc aacccgccac ggcctcactc
     2041 acggcaagac catctacttc ttcgacccgt ccggtaaccg caacgaagtg ttctgcgggg
     2101 gagattacaa ctacccggac cacaaaccgg tgacctggac caccgaccag ctgggcaagg
     2161 cgatctttta ccacgaccgc attctcaacg aacgattcat gaccgtgctg acctgataag
     2221 gatccaaact cgagtaagga tctccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2281 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2341 ctcaccttcg ggtgggcctt tctgcgttta tacctagggt acggttttgg gatccttact
     2401 cgagtctaga ctgcagttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat
     2461 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
     2521 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     2581 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     2641 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     2701 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
     2761 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     2821 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     2881 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     2941 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     3001 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     3061 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     3121 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     3181 atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     3241 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
     3301 gcctccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     3361 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tatt
//
