LOCUS       sequence-266733-        2601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluoride riboswitch-regulated 3-way junction dimeric broccoli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Thavarajah W, Silverman AD, Verosloff M, Kelley-Loughnane N, Jewett 
            MC, Lucks JB
  TITLE     Point-of-Use Detection of Environmental Fluoride via a Cell-Free 
            Riboswitch-Based Biosensor.
  JOURNAL   ACS Synth Biol. 2019 Dec 12. doi: 10.1021/acssynbio.9b00347.
  PUBMED    31829623
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Dec 12. doi: 10.1021/acssynbio.9b00347."
COMMENT     Sequence Label: pJBL7026
FEATURES             Location/Qualifiers
     source          1..2601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        69..103
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     terminator      346..417
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      433..460
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             616..1275
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(682..701)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1296..1390
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1797..1814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1931..2476)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2011..2030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgataagct tccgatggcg cgccgagagg ctttacactt tatgcttccg gctgaattct
       61 aaagatcttt gacagctagc tcagtcctag gtataatact agtttatagg cgatggagtt
      121 cgccataaac gctgcttagc taatgactcc taccagtatc actactggta ggagtctatt
      181 tttttcccac atactctgat gatccgagac ggtcgggtcc agatattcgt atctgtcgag
      241 tagagtgtgg gctcggatca ttcatggcaa gagacggtcg ggtccagata ttcgtatctg
      301 tcgagtagag tgtgggctct tgccatgtgt atgtgggcca ggcatcaaat aaaacgaaag
      361 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      421 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataccta gggtacggtt
      481 ttgggatcct tactcgagtc tagactgcag ttgatcgggc acgtaagagg ttccaacttt
      541 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
      601 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      661 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      721 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      781 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg
      841 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      901 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      961 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     1021 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     1081 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     1141 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     1201 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     1261 cagggcgggg cgtaatttga tatcgagctc gcttggactc ctgttgatag atccagtaat
     1321 gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat
     1381 tggtgagaat ccaagcctcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt
     1441 cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg
     1501 tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt agtgtatgat
     1561 ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta
     1621 ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag cggagtgtat
     1681 actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga
     1741 aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg
     1801 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg
     1861 ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg
     1921 caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa
     1981 atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct
     2041 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     2101 cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     2161 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt
     2221 cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga
     2281 tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt
     2341 tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac
     2401 cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag
     2461 accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct agatttcagt
     2521 gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata agttgtaatt
     2581 ctcatgtttg acagcttatc a
//
