LOCUS       sequence-266752-        6180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allelic exchange plasmid to to integrate firefly luciferase gene 
            into S. pyogenes for biophotonic imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Loh JM, Proft T
  TITLE     Toxin-antitoxin-stabilized reporter plasmids for biophotonic imaging
            of Group A streptococcus.
  JOURNAL   Appl Microbiol Biotechnol. 2013 Nov;97(22):9737-45. doi: 
            10.1007/s00253-013-5200-7. Epub 2013 Sep 6.
  PUBMED    24061415
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-013-5200-7"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2013-11"; volume: "97"; issue: "22"; pages: 
            "9737-45"
COMMENT     Sequence Label: pFW11-ffluc
FEATURES             Location/Qualifiers
     source          1..6180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1653
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(67..85)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     rep_origin      complement(2996..3584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3076..3095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga
       61 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      121 gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc
      181 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      241 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      301 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt
      361 tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
      421 aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga
      481 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
      541 tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga
      601 tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg
      661 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
      721 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
      781 cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac
      841 aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg
      901 attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg
      961 aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat
     1021 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1081 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1141 acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt
     1201 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1261 ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct
     1321 ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa
     1381 caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt
     1441 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     1501 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     1561 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     1621 aaggccaaga agggcggaaa gtccaaattg taaggatcaa ttcaaaaatt gaaaaaatgg
     1681 tggaaacact ttcttcaatt tttttgtttt attatttaat atttgggaaa tattcattct
     1741 aattggtaag cgcgccatgg gccctgcagg agtatattaa tggaggctaa aatgaatttg
     1801 agtatatttg gtttaaagaa tattccttat ttgaaagaag gggatagtat agaaaagctt
     1861 attgaagaaa gcattaaaac ttcagaattt tttatcgaag acaatgatgt gctttgcata
     1921 gcttctaaag ttgtatctat cgcagaaggc caggtgatgt ctttaaacga gatacaagtg
     1981 agtgatgtag caaaagaaat ccacaggaat attcctcgta aagatcctag aataattgaa
     2041 attatgctaa atttagtcaa tagagatctg tcacgtttag atatcaaaaa aaattacatt
     2101 ggttgtcgct tggaaaatgg tttaaagttg acaagtggtg gaatcgatag aaaatcagta
     2161 gatgaagtat ttcttttacc taataatccg gatgcttctg caaaaagaat ttcggaatat
     2221 cttaaaaaat cacttggaaa aaatgttgca gttgttataa cagatagtga tggtagagag
     2281 gataaaagag gtgctactca agtagcaatt ggtatttatg gtattcatcc tttgagaaaa
     2341 acagaagtaa tagattcaca aggagagaca ataaaattcc aagaagagac tttgtgtgat
     2401 atgattgcag cttgtgctgg cttagtaatg gggcaacgtg gaactggaat tccagctgtc
     2461 ctcattaggg gcttggatta taaatggtct gaaaatacta gtattaagga agcattaaat
     2521 gagtagaaat caggactgta agaatattct aatcattggt tttggaaaaa ttgggaaaat
     2581 taaagcgtac aagtggctat cacgcggata tgaggtatat gtcaaagatt tgaaattctc
     2641 cagcatgaaa taccttaata caaatagtga tgtgttggat gatttatcac gacaatattt
     2701 tactattgag atttgtactc cgacaaatca ccaccttacg ccccgggcgc cggcctagga
     2761 ctagtgatca ccggtcgctc ttccgcttcc tcgctcactg acgcgtaaaa aaggcccaca
     2821 aaagtgggcc ttttttctcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3421 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccctcg
     3481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3541 ttgtttgcaa gcagcagatt acgtgcagaa aaaaaggatc tcaagaagat cctttgatct
     3601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3661 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3721 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3781 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3841 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3901 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3961 gaagtggtcc gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta
     4021 tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta
     4081 ccgagaaaaa aagcggccaa aaaggcctgc caccaggcca aaaaggccgc ttttttcgaa
     4141 gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac tgataagata
     4201 atatttcttt tatatagaag attttcgttc gtgaatacat tgtacaattg ctagcgtacg
     4261 gccgtcgacg tccggatccg ggaagttact taggtgtaca cttaagtctt tagagtatct
     4321 aaagacttat aggtaatggt tagctctgtg taatgcttgt actttatgtt agtagtaaac
     4381 taattaaacg taggaggatg aaatttatgt attctgcatt gcttggtaat ccagttgaac
     4441 attctatatc agatgaatta tttgaatatc taaatcacaa cataggagtt ctggatgaat
     4501 ataaacataa aaaaattttg gtagactcag tgtgtttaga agataaaatc aaacatctga
     4561 taaatgatcc aaaatgtatt ggattaaata taactttacc ttataaaaga gaggtaatga
     4621 ggtatattga caatttagac agagtagcag agataactgg atctgttaac aatatttata
     4681 aatctggaaa tataattaca ggaactaata ctgattggaa aggtatttat aatacattag
     4741 ttttctttaa tattagccat ataacaaaat gttgtgtttt aggtactggc ggtactgcta
     4801 gagctgctat tttcacttta caaaaactac aaatctcacc aacagttgta tacagggagc
     4861 caattagtga gaatacaaga agtttgataa agagtttccc tgatttaaag tatattacat
     4921 ataaggaatt ggctgatcaa caaattttaa atgatatgga aatcataata aatactacac
     4981 cagttggtat gataaattat gaagaaagtt tacctataaa ttttgatcat ttggacaatg
     5041 ttaattataa agataaatac tttatggatg tagttttcaa tcctttacat acgaaacttc
     5101 atgaatattt tggtaaaaaa ggagcaaaaa taattgatgg tttatgggtt ttgatttttc
     5161 agggaattga agcattttca ttgtggagtg ataaaattaa tgatggtaaa tatttttttt
     5221 caaaagatga tgttgtccta attcatacat ttctagaagg gaagataaaa gaagcttgat
     5281 tttcgttcgt gaatacatgt tataataact ataactaata acgtaacgta aaatttgttt
     5341 gatttgtcca aaatatggta taataggtac taatcaaaat agtgaggagg atatatttga
     5401 atacatacga acaaattaat aaagtgaaaa aaatacttcg gaaacattta aaaaataacc
     5461 ttattggtac ttacatgttt ggatcaggag ttgagagtgg actaaaacca aatagtgatc
     5521 ttgacttttt agtcgtcgta tctgaaccat tgacagatca aagtaaagaa atacttatac
     5581 aaaaaattag acctatttca aaaaaaatag gagataaaag caacttacga tatattgaat
     5641 taacaattat tattcagcaa gaaatggtac cgtggaatca tcctcccaaa caagaattta
     5701 tttatggaga atggttacaa gagctttatg aacaaggata cattcctcag aaggaattaa
     5761 attcagattt aaccataatg ctttaccaag caaaacgaaa aaataaaaga atatacggaa
     5821 attatgactt agaggaatta ctacctgata ttccattttc tgatgtgaga agagtcatta
     5881 tggattcgtc agaggaatta atagataatt atcaggatga tgaaaccaac tctatattaa
     5941 ctttatgccg tatgatttta actatggaca cgggtaaaat cataccaaaa gatattgcgg
     6001 gaaatgcagt ggctgaatct tctccattag aacataggga gagaattttg ttagcagttc
     6061 gtagttatct tggagagaat attgaatgga ctaatgaaaa tgtaaattta actataaact
     6121 atttaaataa cagattaaaa aaattataag aatcggaatt gatctaagga ggaataaaaa
//
