LOCUS       sequence-26676-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Enslen H, Brancho DM, Davis RJ
  TITLE     Molecular determinants that mediate selective activation of p38 MAP 
            kinase isoforms.
  JOURNAL   EMBO J. 2000 Mar 15. 19(6):1301-11.
  PUBMED    10716930
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2000 Mar 15. 19(6):1301-11."
COMMENT     Sequence Label: pCDNA3 Flag p38 delta
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..107
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt atggactaca aggacgacga tgacaagagc ctcactcgga
      121 aaaggggctt ctacaagcag gacatcaaca agactgcctg ggagctaccc aagacctacc
      181 tggcgcccgc gcacgtcggc agcggggcct atggagcggt gtgctcggcc atcgacaagc
      241 ggacagggga gaaggtggcc atcaagaagc tgagccggcc cttccagtcg gagatctttg
      301 ccaagcgcgc ttaccgcgag cttctgctct tgaagcacat gcaccatgag aacgtcattg
      361 ggcttctgga tgtcttcacc cctgcgtctt cccttcggag cttccatgat ttctacctgg
      421 tgatgccctt catgcagaca gacctacaga agatcatggg gatggaattc agcgaggata
      481 aggtccagta cttggtgtac cagatgctca aaggtctaaa gtacatccac tccgctggca
      541 tcgtccacag ggacctgaaa ccgggcaacc tggctgtgaa tgaagactgt gagctgaaga
      601 tcctggactt tgggctggcc cgccacacag acactgagat ganngggcta tgtggtgacc
      661 cgctggtacc gggcccccga ggtgatcctc agctggatgc attacaacca gacagtcgac
      721 ntctggtctg ntggttgcat c
//
