LOCUS       sequence-266771-        1311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E. Coli TGT with a C-terminal His Tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Busby KN, Devaraj NK
  TITLE     Enzymatic covalent labeling of RNA with RNA transglycosylation at 
            guanosine (RNA-TAG).
  JOURNAL   Methods Enzymol. 2020;641:373-399. doi: 10.1016/bs.mie.2020.03.009. 
            Epub 2020 Apr 20.
  PUBMED    32713531
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2020"; volume: "641:373-399. doi"; pages: " 
            10.1016/bs.mie.2020.03.009. Epub 2020 Apr 20"
COMMENT     Sequence Label: TGT-His
FEATURES             Location/Qualifiers
     source          1..1311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             85..107
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1237..1254
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctgcagggcc agtgaattcg agctcggtac ctcgcgaatg catctagata tcggatccta
       61 atacgactca ctatagggaa taattttgtt taactttaag aaggagatat aatgaaattt
      121 gaactggaca ccaccgacgg tcgcgcacgc cgtggccgcc tggtctttga tcgtggcgta
      181 gtggaaacgc cttgttttat gcctgttggc acctacggca ccgtaaaagg gatgacgccg
      241 gaagaagttg aagccactgg cgcgcaaatt atcctcggca acaccttcca cctgtggctg
      301 cgcccgggcc aggaaatcat gaaactgcac ggcgatctgc acgattttat gcagtggaag
      361 gggccgatcc tcaccgactc cggcggcttc caggtcttca gccttggcga tattcgtaaa
      421 atcaccgaac agggcgtgca cttccgtaac ccgatcaacg gcgatccgat tttcctcgat
      481 cctgaaaaat caatggagat tcagtacgat cttggttcgg atatcgtcat gatctttgat
      541 gagtgtacgc cgtatcctgc tgactgggat tacgcaaaac gctccatgga gatgtctctg
      601 cgttgggcga agcgtagccg tgagcgtttt gacagtctcg gaaacaaaaa tgcgctgttt
      661 ggtatcatcc agggcagcgt ttacgaagat ttacgtgata tttctgttaa aggtctggta
      721 gatatcggtt ttgatggcta cgctgtcggc ggtctggctg tgggtgagcc gaaagcagat
      781 atgcaccgca ttctggagca tgtatgcccg caaattccgg cagacaaacc gcgttacctg
      841 atgggcgttg gtaaaccaga agacctggtt gaaggcgtac gtcgtggtat cgatatgttt
      901 gactgcgtaa tgccaacccg caacgcccga aatggtcatt tgttcgtgac cgatggcgtg
      961 gtgaaaatcc gcaatgcgaa gtataagagc gatactggcc cactcgatcc tgagtgtgat
     1021 tgctacacct gtcgcaatta ttcacgcgct tacttgcatc atcttgaccg ttgcaacgaa
     1081 atattaggcg cgcgactcaa caccattcat aaccttcgtt actaccagcg tttgatggcg
     1141 ggtttacgca aggctattga agagggtaaa ttagagagct tcgtaactga tttttaccag
     1201 cgtcaggggc gagaagtacc acctttgaac gttgatcacc atcaccacca tcactaaaaa
     1261 ggcgggcctc gagcaaagcc cgccgaaagg cgggcttttc tgtgtaagct t
//
