LOCUS       sequence-266778-        6287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human GATA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Ko LJ, Yamamoto M, Leonard MW, George KM, Ting P, Engel JD
  TITLE     Murine and human T-lymphocyte GATA-3 factors mediate transcription 
            through a cis-regulatory element within the human T-cell receptor 
            delta gene enhancer.
  JOURNAL   Mol Cell Biol. 1991 May . 11(5):2778-84.
  PUBMED    2017177
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1991 May . 11(5):2778-84."
COMMENT     Sequence Label: RSV/hGATA3
FEATURES             Location/Qualifiers
     source          1..6287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..268)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     545..564
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(645..662)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(816..1404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(896..915)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1575..2366,2367..2435))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2198..2217
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2436..2540)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2608..2626
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    3393..3527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3417..3436)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3471..3490
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3952..3972)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4102..4167
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 cttggaggtg cacaccaatg tggtgaatgg tcaaatggcg tttattgtat cgagctaggc
       61 acttaaatac aatatctctg caatgcggaa ttcagtggtt cgtccaatcc atgtcagacc
      121 cgtctgttgc cttcctaata aggcacgatc gtaccacctt acttccacca atcggcatgc
      181 acggtgcttt ttctctcctt gtaaggcatg ttgctaactc atcgttacca tgttgcaaga
      241 ctacaagagt attgcataag actacatttc cccctcccta tgcaaaagcg aaactactat
      301 atcctgaggg gactcctaac cgcgtacaac cgaagccccg cttttcgcct aaacacaccc
      361 tagtcccctc agatacgcgt atatctggcc cgtacatcgc gaagcagcgc aaaacgccta
      421 accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt
      481 ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata ctggcttaac
      541 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
      601 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
      661 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      721 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      781 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      841 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      901 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      961 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1021 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1081 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1141 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1201 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     1261 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1321 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1381 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1441 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1501 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1561 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1621 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1681 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1741 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1801 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1861 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1921 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1981 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2041 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2101 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2161 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     2221 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2281 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2341 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2401 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2461 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2521 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2581 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2641 gaattccttt gcctaattta aatgaggact taacctgtgg aaatattttg atgtgggaag
     2701 ctgttactgt taaaactgag gttattgggg taactgctat gttaaacttg cattcaggga
     2761 cacaaaaaac tcatgaaaat ggtgctggaa aacccattca agggtcaaat tttcattttt
     2821 ttgctgttgg tggggaacct ttggagctgc agggtgtgtt agcaaactac aggaccaaat
     2881 atcctgctca aactgtaacc ccaaaaaatg ctacagttga cagtcagcag atgaacactg
     2941 accacaaggc tgttttggat aaggataatg cttatccagt ggagtgctgg gttcctgatc
     3001 caagtaaaaa tgaaaacact agatattttg gaacctacac aggtggggaa aatgtgcctc
     3061 ctgttttgca cattactaac acagcaacca cagtgcttct tgatgagcag ggtgttgggc
     3121 ccttgtgcaa agctgacagc ttgtatgttt ctgctgttga catttgtggg ctgtttacca
     3181 acacttctgg aacacagcag tggaagggac ttcccagata ttttaaaatt acccttagaa
     3241 agcggtctgt gaaaaacccc tacccaattt cctttttgtt aagtgaccta attaacagga
     3301 ggacacagag ggtggatggg cagcctatga ttggaatgtc ctctcaagta gaggaggtta
     3361 gggtttatga ggacacagag gagcttcctg gggatccaga catgataaga tacattgatg
     3421 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     3481 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     3541 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     3601 acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat acatcaaata
     3661 ttccttatta acccctttac aaattaaaaa gctaaaggta cacaattttt gagcatagtt
     3721 attaatagca gacactctat gcctgtgtgg agtaagaaaa aacagtatgt tatgattata
     3781 actgttatgc ctacttataa aggttacaga atatttttcc ataattttct tgtatagcag
     3841 tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca agagttctat tactaaacac
     3901 agcatgactc aaaaaactta gcaattctga aggaaagtcc ttggggtctt ctacctttct
     3961 cttctttttt ggaggagtag aatgttgaga gtcagcagta gcctcatcat cactagatgg
     4021 catttcttct gagcaaaaca ggttttcctc attaaaggca ttccaccact gctcccattc
     4081 atcagttcca taggttggaa tctaaaatac acaaacaatt agaatcagta gtttaacaca
     4141 ttatacactt aaaaatttta tatttacctt agagctttaa atctctgtag gtagtttgtc
     4201 caattatgtc acaccacaga agtaaggttc cttcacaaag atcaattccc ttttttttat
     4261 tttcttttaa tgcatcaaac aactgtggcc agtgaaagga aacaaaactg gcagtttgtc
     4321 catttgaata tcagacctag tttcttctta atttccacac catttctccc atattcctta
     4381 aacttcttgg catccttcat gccttacagc tacccagatg caataaagtc attgtacagt
     4441 atttcttaca atataagtta tatgcaatgt tcagcatttt tttttttcac agcactagag
     4501 accctgttaa ataggggata tgagtcagaa tggcttattc acagatgggg tccagattca
     4561 gtggttggaa cacagacacc acagtgagct cctttgcaaa gtggcaaaca taattttgct
     4621 ttctgccttc aaaaacatat atccatcgcg tttaggcttc atgatactgc tcctgcaaaa
     4681 atgcaagtcg aaagggactg cagggactct cgctgggggc cctgtgagca tcgagcaggg
     4741 ctctaaccca tggcggtgac catgctggag gggtggtgtg gtccaaagga caggctggat
     4801 ggcgggtgca tcggcgtggg cgtggtcagc atgtggctgg agtggctgaa gggcgagatg
     4861 tggctcaggg aggacatgtg tctggagagg gcggccgggt taaacgagct gttcttgggg
     4921 aagtcctcca gtgagtcatg cacttttttg cactttttgg atttgctaga catttttcgg
     4981 tttctggtct ggatgccttc cttcttcata gtcaggggtc tgttaatatt gtgaagcttg
     5041 tagtagagcc cacaggcatt gcagacaggg tccccattgg cattcctcct ccagagtgtg
     5101 gttgtggtgg tctgacagtt cgcacaggac gtccctgctc tcctggctgc agacagcctt
     5161 cgcttgggct taatgagggg ccggttctgt ccgttcattt tgtgatagag cccgcaggcg
     5221 ttgcacaggt agtgtcccgt gccatctcgc cgccacagtg gggtcgaggt tgccccacag
     5281 ttcacacact ccctgccttc tgtgctggac cgggccttgg gcctggactt gcatccgaag
     5341 ccggtggggg agccgcccag caggctgctg ggggggaaga gtccggagct gtactcgggc
     5401 acgtagggcg ggtaggtggt gatggggtgg tgggtcgacg aggaggctcc acccagggcg
     5461 gtcatgctgc cacgggagtg ggacgactcc agcttcatgc tgtcgggcag gggcacctgg
     5521 tacttgaggc actctttctc gtcctgccgg gccgagccgg ccgagcctgg ggtggacagc
     5581 gatgggtccg gggagacgtc cttcggcggg gtgggcggga aggtgaagag gtgcgggctg
     5641 gcgtggcccc ccgacaagga ggaggacgag gccggggggt agacggagag gggccccggg
     5701 gagccgtggt ggatggacgt cttggagaag gggctgagat tccaggggga ggcggtgtgg
     5761 tggctgccca gggctttgcc gccgtccagc cagggtaggg atccatgaag cagaggcggg
     5821 cggcacacct ggctcccgtg gtgggtcgga gggtacctct gcaccgtggc cctgaccgag
     5881 tttccgtagt agggcgggac gtggttgcct tgaccgtcga tgttaaaaag cacatccacc
     5941 tcctccggca gcgggtactg cgccgcgtcc atgtaggagt ggctgaggcc cgggtggtgc
     6001 gtgtccgggt gctgcccgtt gagcacggcg gggtggtggt ggctcaccca gcgcggctgg
     6061 tccgccgtca cctccatggc ctcggctgtg ctcgcgccct ctcgccgggc ccggaacccg
     6121 cgcgcgggga gggagggcgg tccgcctggg aggtcggggg gctcctgccg tcggagggtc
     6181 gggggtcgtt gaatgatttg ctttcggtgg ggggatggga aggctgggaa gcaaaggtga
     6241 gcaaaggaga aggtttttta aaaattatct tggagggggg gaattag
//
