LOCUS       sequence-266824-       11899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 
            gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version
            of pAH-CTX1-rhadCas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11899)
  AUTHORS   Hogan AM, Rahman ASMZ, Lightly TJ, Cardona ST
  TITLE     A broad-host-range CRISPRi toolkit for silencing gene expression in 
            Burkholderia.
  JOURNAL   ACS Synth Biol. 2019 Sep 6. doi: 10.1021/acssynbio.9b00232.
  PUBMED    31491085
REFERENCE   2  (bases 1 to 11899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Sep 6. doi: 10.1021/acssynbio.9b00232."
COMMENT     Sequence Label: pAH-CTX1-rhadCas9-native
FEATURES             Location/Qualifiers
     source          1..11899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    195..242
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     416..435
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        416..434
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     459..475
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     460..476
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(520..1368)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(1442..2278)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLDQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     promoter        2424..2542
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             join(2566..2592,2593..2595,2596..5082,5083..5085,
                     5086..6672)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      6696..6767
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6783..6810
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(6848..6868)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6848..6866)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    7405..7452
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(7671..7676,7677..7693,7694..7699)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             7747..8937
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(8099..8118)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      9759..10347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10248..10267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            11779..11887
                     /note="incP origin of transfer"
ORIGIN
        1 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
       61 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      121 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      181 gaattgggga tcttgaagta cctattccga agttcctatt ctctagaaag tataggaact
      241 tcagagcgct tttgaagctg atgtgcttaa aaacttactc aatggaatta gctttatgct
      301 tgtaaaccgt tttgtgaaaa aatttttaaa ataaaaaagg ggacctctag ggtccccaat
      361 taattagtaa tataatctat taaaggtcat tcaaaaggtc atccaccggc gcgcgtaata
      421 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa
      481 gcttatgtca actgggttcg tgcgagctca tcgatgcatt taatctttct gcgaattgag
      541 atgacgccac tggctgggcg tcatcccggt ttcccgggta aacaccaccg aaaaatagtt
      601 actatcttca aagccacatt cggtcgaaat atcactgatt aacaggcggc tatgctggag
      661 aagatattgc gcatgacaca ctctgacctg tcgcagatat tgattgatgg tcattccagt
      721 ctgctggcga aattgctgac gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt
      781 atccagcgca aagggacttt tcaggctagc cgccagccgg gtaatcagct tatccagcaa
      841 cgtttcgctg gatgttggcg gcaacgaatc actggtgtaa cgatggcgat tcagcaacat
      901 caccaactgc ccgaacagca actcagccat ttcgttagca aacggcacat gctgactact
      961 ttcatgctca agctgaccga taacctgccg cgcctgcgcc atccccatgc tacctaagcg
     1021 ccagtgtggt tgccctgcgc tggcgttaaa tcccggaatc gccccctgcc agtcaagatt
     1081 cagcttcaga cgctccgggc aataaataat attctgcaaa accagatcgt taacggaagc
     1141 gtaggagtgt ttatcgtcag catgaatgta aaagagatcg ccacgggtaa tgcgataagg
     1201 gcgatcgttg agtacatgca ggccattacc gcgccagaca atcaccagct cacaaaaatc
     1261 atgtgtatgt tcagcaaaga catcttgcgg ataacggtca gccacagcga ctgcctgctg
     1321 gtcgctggca aaaaaatcat ctttgagaag ttttaactga tgcgccaccg tggctacctc
     1381 ggccagagaa cgaagttgat tattcgcaat atggcgtaca aatacgttga gaagattcgc
     1441 gttattgcag aaagccatcc cgtccctggc gaatatcacg cggtgaccag ttaaactctc
     1501 ggcgaaaaag cgtcgaaaag tggttactgt cgctgaatcc acagcgatag gcgatgtcag
     1561 taacgctggc ctcgctgtgg cgtagcagat gtcgggcttt catcagtcgc aggcggttca
     1621 ggtatcgctg aggcgtcagt cccgtttgct gcttaagctg ccgatgtagc gtacgcagtg
     1681 aaagagaaaa ttgatccgcc acggcatccc aattcacctc atcggcaaaa tggtcctcca
     1741 gccaggccag aagcaagttg agacgtgatg cgctgttttc caggttctcc tgcaaactgc
     1801 ttttacgcag caagagcagt aattgcataa acaagatctc gcgactggcg gtcgagggta
     1861 aatcattttc cccttcctgc tgttccatct gtgcaaccag ctgtcgcacc tgctgcaata
     1921 cgctgtggtt aacgcgccag tgagacggat actgcccatc cagctcttgt ggcagcaact
     1981 gatccagccc ggcgagaaac tgaaatcgat ccggcgagcg atacagcaca ttggtcagac
     2041 acagattatc ggtatgttca tacagatgcc gatcatgatc gcgtacgaaa cagaccgtgc
     2101 caccggtgat ggtatagggc tgcccattaa acacatgaat acccgtgcca tgttcgacaa
     2161 tcacaatttc atgaaaatca tgatgatgtt caggaaaatc cgcctgcggg agccggggtt
     2221 ctatcgccac ggacgcgtta ccagacggaa aaaaatccac actatgtaat acggtcatac
     2281 tggcctcctg atgtcgtcaa cacggcgaaa tagtaatcac gaggtcaggt tcttacctta
     2341 aattttcgac ggaaaaccac gtaaaaaacg tcgatttttc aagatacagc gtgaattttc
     2401 aggaaatgcg gtgagcatca catcaccaca attcagcaaa ttgtgaacat catcacgttc
     2461 atctttccct ggttgccaat ggcccatttt cctgtcagta acgagaaggt cgcgaattca
     2521 ggcgcttttt agactggtcg taatgaaatt cagcaggaac tagttatgga taagaaatac
     2581 tcaataggct tagctatcgg cacaaatagc gtcggatggg cggtgatcac tgatgaatat
     2641 aaggttccgt ctaaaaagtt caaggttctg ggaaatacag accgccacag tatcaaaaaa
     2701 aatcttatag gggctctttt atttgacagt ggagagacag cggaagcgac tcgtctcaaa
     2761 cggacagctc gtagaaggta tacacgtcgg aagaatcgta tttgttatct acaggagatt
     2821 ttttcaaatg agatggcgaa agtagatgat agtttctttc atcgacttga agagtctttt
     2881 ttggtggaag aagacaagaa gcatgaacgt catcctattt ttggaaatat agtagatgaa
     2941 gttgcttatc atgagaaata tccaactatc tatcatctgc gaaaaaaatt ggtagattct
     3001 actgataaag cggatttgcg cttaatctat ttggccttag cgcatatgat taagtttcgt
     3061 ggtcattttt tgattgaggg agatttaaat cctgataata gtgatgtgga caaactattt
     3121 atccagttgg tacaaaccta caatcaatta tttgaagaaa accctattaa cgcaagtgga
     3181 gtagatgcta aagcgattct ttctgcacga ttgagtaaat caagacgatt agaaaatctc
     3241 attgctcagc tccccggtga gaagaaaaat ggcttatttg ggaatctcat tgctttgtca
     3301 ttgggtttga cccctaattt taaatcaaat tttgatttgg cagaagatgc taaattacag
     3361 ctttcaaaag atacttacga tgatgattta gataatttat tggcgcaaat tggagatcaa
     3421 tatgctgatt tgtttttggc agctaagaat ttatcagatg ctattttact ttcagatatc
     3481 ctaagagtaa atactgaaat aactaaggct cccctatcag cttcaatgat taaacgctac
     3541 gatgaacatc atcaagactt gactctttta aaagctttag ttcgacaaca acttccagaa
     3601 aagtataaag aaatcttttt tgatcaatca aaaaacggat atgcaggtta tattgatggg
     3661 ggagctagcc aagaagaatt ttataaattt atcaaaccaa ttttagaaaa aatggatggt
     3721 actgaggaat tattggtgaa actaaatcgt gaagatttgc tgcgcaagca acggaccttt
     3781 gacaacggct ctattcccca tcaaattcac ttgggtgagc tgcatgctat tttgagaaga
     3841 caagaagact tttatccatt tttaaaagac aatcgtgaga agattgaaaa aatcttgact
     3901 tttcgaattc cttattatgt tggtccattg gcgcgtggca atagtcgttt tgcatggatg
     3961 actcggaagt ctgaagaaac aattacccca tggaattttg aagaagttgt cgataaaggt
     4021 gcttcagctc aatcatttat tgaacgcatg acaaactttg ataaaaatct tccaaatgaa
     4081 aaagtactac caaaacatag tttgctttat gagtatttta cggtttataa cgaattgaca
     4141 aaggtcaaat atgttactga aggaatgcga aaaccagcat ttctttcagg tgaacagaag
     4201 aaagccattg ttgatttact cttcaaaaca aatcgaaaag taaccgttaa gcaattaaaa
     4261 gaagattatt tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg agttgaagat
     4321 agatttaatg cttcattagg tacctaccat gatttgctaa aaattattaa agataaagat
     4381 tttttggata atgaagaaaa tgaagatatc ttagaggata ttgttttaac attgacctta
     4441 tttgaagata gggagatgat tgaggaaaga cttaaaacat atgctcacct ctttgatgat
     4501 aaggtgatga aacagcttaa acgtcgccgt tatactggtt ggggacgttt gtctcgaaaa
     4561 ttgattaatg gtattaggga taagcaatct ggcaaaacaa tattagattt tttgaaatca
     4621 gatggttttg ccaatcgcaa ttttatgcag ctgatccatg atgatagttt gacatttaaa
     4681 gaagacattc aaaaagcaca agtgtctgga caaggcgata gtttacatga acatattgca
     4741 aatttagctg gtagccctgc tattaaaaaa ggtattttac agactgtaaa agttgttgat
     4801 gaattggtca aagtaatggg gcggcataag ccagaaaata tcgttattga aatggcacgt
     4861 gaaaatcaga caactcaaaa gggccagaaa aattcgcgag agcgtatgaa acgaatcgaa
     4921 gaaggtatca aagaattagg aagtcagatt cttaaagagc atcctgttga aaatactcaa
     4981 ttgcaaaatg aaaagctcta tctctattat ctccaaaatg gaagagacat gtatgtggac
     5041 caagaattag atattaatcg tttaagtgat tatgatgtcg atgccattgt tccacaaagt
     5101 ttccttaaag acgattcaat agacaataag gtcttaacgc gttctgataa aaatcgtggt
     5161 aaatcggata acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta ttggagacaa
     5221 cttctaaacg ccaagttaat cactcaacgt aagtttgata atttaacgaa agctgaacgt
     5281 ggaggtttga gtgaacttga taaagctggt tttatcaaac gccaattggt tgaaactcgc
     5341 caaatcacta agcatgtggc acaaattttg gatagtcgca tgaatactaa atacgatgaa
     5401 aatgataaac ttattcgaga ggttaaagtg attaccttaa aatctaaatt agtttctgac
     5461 ttccgaaaag atttccaatt ctataaagta cgtgagatta acaattacca tcatgcccat
     5521 gatgcgtatc taaatgccgt cgttggaact gctttgatta agaaatatcc aaaacttgaa
     5581 tcggagtttg tctatggtga ttataaagtt tatgatgttc gtaaaatgat tgctaagtct
     5641 gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt actctaatat catgaacttc
     5701 ttcaaaacag aaattacact tgcaaatgga gagattcgca aacgccctct aatcgaaact
     5761 aatggggaaa ctggagaaat tgtctgggat aaagggcgag attttgccac agtgcgcaaa
     5821 gtattgtcca tgccccaagt caatattgtc aagaaaacag aagtacagac aggcggattc
     5881 tccaaggagt caattttacc aaaaagaaat tcggacaagc ttattgctcg taaaaaagac
     5941 tgggatccaa aaaaatatgg tggttttgat agtccaacgg tagcttattc agtcctagtg
     6001 gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat ccgttaaaga gttactaggg
     6061 atcacaatta tggaaagaag ttcctttgaa aaaaatccga ttgacttttt agaagctaaa
     6121 ggatataagg aagttaaaaa agacttaatc attaaactac ctaaatatag tctttttgag
     6181 ttagaaaacg gtcgtaaacg gatgctggct agtgccggag aattacaaaa aggaaatgag
     6241 ctggctctgc caagcaaata tgtgaatttt ttatatttag ctagtcatta tgaaaagttg
     6301 aagggtagtc cagaagataa cgaacaaaaa caattgtttg tggagcagca taagcattat
     6361 ttagatgaga ttattgagca aatcagtgaa ttttctaagc gtgttatttt agcagatgcc
     6421 aatttagata aagttcttag tgcatataac aaacatagag acaaaccaat acgtgaacaa
     6481 gcagaaaata ttattcattt atttacgttg acgaatcttg gagctcccgc tgcttttaaa
     6541 tattttgata caacaattga tcgtaaacga tatacgtcta caaaagaagt tttagatgcc
     6601 actcttatcc atcaatccat cactggtctt tatgaaacac gcattgattt gagtcagcta
     6661 ggaggtgact aactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
     6721 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     6781 ctggctcacc ttcgggtggg cctttctgcg tgcggccgcc accgcggtgg agctccagct
     6841 tttgttccct ttagtgaggg ttaattgcgc gggtggatga ccttttgaat gacctttaat
     6901 agattatatt actaattaat tggggaccct agaggtcccc ttttttattt taaaaatttt
     6961 ttcacaaaac ggtttacaag cataaagcta attcagcaag gacggccccg acatcgtagg
     7021 tctgctgaat caggcgctga aactttcctg aagcaacgaa aaaggggccc tgcggggccc
     7081 cttttttctg cgcctcgttc ccagtttgtt cccaaatcgt tcactcctga gagtgggtcg
     7141 aatggaaaca tcaatgaaaa cagcaggtta tatggcggag gcggtgagat tcgaactcat
     7201 atgaaacgcc ccaacctcgc tggaggccgc attattcctg ggccctactg tccattttca
     7261 ctcgcgtttg ttcccagagt gttcccagcg tgtgcgagag agcctggaag cctgccgtca
     7321 aacctgccgt cccagggcaa cagccggatt tcatgttgac ccattgaaat ccattgctgt
     7381 tgacaaaggg aatcagggga tcttgaagtt cctattccga agttcctatt ctctagaaag
     7441 tataggaact tcagagcgct tttgaagcta attcgctgca ttaatgaatc ggccaacgcg
     7501 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7621 ccacagaatc aggggataac gcaggaaaga acatgaatta attctcatgt ttgacagctt
     7681 atcatcgatt agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca
     7741 ccgtgtatga aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct
     7801 gtaggcatag gcttggttat gccggtactg ccgggcctct tgcgggatat tgtccattcc
     7861 gacagcatcg ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta
     7921 tgcgcacccg ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct
     7981 tcgctacttg gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatt
     8041 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     8101 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     8161 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     8221 ttacatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     8281 ttcctaatgc aggagtcgca taagggagag cgccgaccca tgcccttgag agccttcaac
     8341 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     8401 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag
     8461 gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg
     8521 cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag
     8581 gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg
     8641 cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc
     8701 gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga
     8761 tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt cacggcgatt
     8821 tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac
     8881 cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg
     8941 gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     9001 tgcggagaac tgtgaatgcg caaaccaacc cttgactcac cagtcacaga aaagcatctt
     9061 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     9121 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     9181 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     9241 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9301 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9361 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9421 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9481 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9541 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9601 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9661 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9721 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9781 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9841 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9901 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9961 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    10021 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    10081 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    10141 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    10201 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10261 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10321 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10381 gccttttgct ggccttttgc tcacatccag cttggtagcc tgaacgcttg gtgtggatca
    10441 ggggaaccag atggcagacg gagtcgaggt acgcggaaaa cggatcagga tctacttccg
    10501 ctaccagggc gagctgtgta gggagtccat accaggggac gcaacgcccg agaatatagc
    10561 caatgccgaa cagctggccg gcatcatcaa ctacgagatc aagcaaggag tcttcagcta
    10621 cagccgacac ttcccggact caccgcgggt gaaaagcaac accttgggcc attacatcga
    10681 cctctggctc gacatcaaac gcaaccagat tgccgccagc ggcttccgcg gctacaccag
    10741 ccgcgtggaa acccacatcc gacctcgctg gggtgacagc caggccgaca gcatcgacca
    10801 cctggatatt caagactggg tgcagaacac gctcatgccc aagctgcaca acaagacggt
    10861 gcgagagatc gtcagcaacc tgcggcagat cttccggctg tatcgaactc ggaaccggag
    10921 cgcgcacgac ccaacagacg gcatcgtgat cactcttccg gatgcggatg acccagaccc
    10981 attcactcgt gaggaaatcg acctcatcct cggtactgaa accgcgcgca tcggtgagct
    11041 gaacctggcg gagttcatga tctggagcgg cccccgcgtc agcgaggcca tagctctggc
    11101 ctgggaagat gtcgatttgg acacggggac cgtggttttc cgccgcgctc gtgtacgcag
    11161 ccagtacaag gtcaccaaga ctcgccgatc gacgaggaag gtccagctac tggccccggc
    11221 gctgcgcgca ctccagcagc aggccaagct aaccagacgc cttccgcccg tacagatcga
    11281 ggtgatagac cgcgacaacc ggaccagaaa gcctcaacgg gtacgcttcg tgttccacaa
    11341 cagtgctagc ggggcggcgt actccacgtc ggatacgctg cgcaacggct ggtggcatgg
    11401 ccacctcagg aatgcgggcg tccgctcgcg tggtcccaat cagtgccgcc acaccttcgc
    11461 cagccagatg ctcagcagcg gcatcgccac tcccgaatgg atcgcggacc agatggggca
    11521 cacctcgacg gcgatgatct tcaagcatta cgccaagtgg atcagcaagg acggccccga
    11581 catcgtaggt ctgctgaatc aggcgctgaa actttcctga agcaacgaaa aaggttaagg
    11641 tatactttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt cggtatatcc
    11701 atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact ttccttggtg
    11761 tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc
    11821 cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg
    11881 ctggccgata agctctagc
//
