LOCUS       sequence-266825-       10403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% 
            glucose, induced with 0.3% xylose; for homologous recombination at 
            the xylose locus in Caulobacter crescentus; tetracycline resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Guzzo M, Castro LK, Reisch CR, Guo MS, Laub MT
  TITLE     A CRISPR Interference System for Efficient and Rapid Gene Knockdown 
            in Caulobacter crescentus.
  JOURNAL   mBio. 2020 Jan 14;11(1):e02415-19. doi: 10.1128/mBio.02415-19.
  PUBMED    31937638
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/mBio"; 
            journalName: "mBio"; date: "2020-01-14- 14"; volume: "11"; issue: 
            "1"; pages: "e02415-19"
COMMENT     Sequence Label: pXGFPC-5 Pxyl-dcas9 (Spy)
FEATURES             Location/Qualifiers
     source          1..10403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     70..86
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      87..133
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             join(2657..2683,2684..2686,2687..5173,5174..5176,
                     5177..6763)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(6915..6931)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6942..6958)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(7156..7744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7236..7255)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7850..7866)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7963..9162)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             9268..9918
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     oriT            10212..10321
                     /note="incP origin of transfer"
ORIGIN
        1 ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa aagatccgtc
       61 gacggccgct ctagaactag tggatccaaa taaaacgaaa ggctcagtcg aaagactggg
      121 cctttcgttt tatctgttgt ttgtcgcatt atacgcaagg cgacaaggtg ctgatgccgc
      181 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
      241 aattacaaca gtttttatat aagcttagcg ctaccggact cagatccaga tcgaaatgta
      301 ccggctgaac tggtggaagc acgttgagcc tcgtcaagga ctcatgaagc gcgtccctgc
      361 tctcagtcgc ctgctcgtta cgcctgaggt gtcaaaacac cgtctgttca tttggctaga
      421 cgctagagtt cttccggatc acaagctcca ggtcgttaca ctcgatgacg actgctcatt
      481 cggagtttta cactcaaggt tccacgaggt gtgggctctt gcagcggggt cgtggcacgg
      541 ctcaggcaat gatccgcgct acaccatctc caccaccttc gagaccttcc ccttcccgga
      601 aggcctgacg cccaacatag ccgccgtcga ctatgagggc gacccgcgcg cccaggccat
      661 cgccgcggcg gcggccgagc tgaaccggct gcgggaagcc tggctcaatc cgcccgacct
      721 ggtgcggatc gagcccgagg tcgtgcccgg ctatcccgac cgcgtgctgc ccgtcagccc
      781 cgaggcgggc gccgagctga agaagcgcac cctgaccaac ctctacaacc agcgcccggc
      841 ctggctggac atggcccacc agcgcctgga cgccgccgtc gccgccgcct acggctggcc
      901 cgacggcctg accgacgacg agatcctgga gcgcctgttc gccctcaacc aggagcgcgc
      961 ggcggcgggg cggtaggtta gacccctcca aaaccccgac ctccccggcg aaggccgggg
     1021 tccagttaca tccggaacgc ttggagtggg cccgcctacg gcctcgacca acccgcagtc
     1081 ggcaaggggt tcgatctgga ccccggcatt cgccggggag gtcggcagag aggtcagaac
     1141 cgcgtcttgc agccctgctt cagatgaacc ccgcgcagga agccgcggcg ggccaggccg
     1201 gcgtcgacgg cggcgttcat ctggcggtcg gcgggggcag cgccggtgac ttcgcgcacc
     1261 aggccccgga acgggatgat gctgttgatc agggtcttgc caccggcctc ggccagcttg
     1321 ccggctcggt tttcggcgta ctcggcgccg accttgtagt cgggccccag cgccgcgttc
     1381 agcgccttca cctcattggc cagggcctgg caggacttca gacccgcgag gctgtagggg
     1441 tcgtcaaagg ccttggccag gaccggcggc acctcgcgct tggacaggcc gacgtcgcgc
     1501 accggctggg tgccgatgtc gacggccttc ttgcccgcct cgccccagcg gccggacggt
     1561 tcctcgggct tggccggcgg cttgacctgg gcgtgggcgc tggaggccag cagcacggcg
     1621 gcggctccgg ccatgatcag cagcttgttc ggcatcaaaa gcgccctccc gacaaaacac
     1681 gccacgggaa cggcaagcct cgccgccggt tcccgcccgc aagtccctcg gcacgaactc
     1741 taaggcctgc gcacgatcca tcgccaccac aaagcgtgtc atcccggccg aagcgcagcg
     1801 gagagccggg acccagggcc acaagcacgg cgcttcgtca ccccctgggt cccggatagc
     1861 gctccgcgct tccgggatga cacgggttta agcacttggg tgagaagccc tagttcggcg
     1921 cgccgcccgc ttccaggtcg aacgcggcgt cgaaggcgtc gtcgccagga accgtccctt
     1981 ccagggcggc gtcgaacagg tcttcgagcg atgaccagat gaaggccatc tgctccatga
     2041 tcgtcgggac gctggccgga tcgaagccgg ggtagaggtc ttccaccagc cacaggcccg
     2101 tgccgggatc gaaggcgaag cggcagccga tcaggcggaa ctgggcgcgg gccatggtct
     2161 cgaacagggc cgtcaggtcg cgggcggcgt ccaggtcgtc gtggtccagc accaggcagc
     2221 ggatctgcag ccagccgtgg tcgggcagca ggtagaaggc gccctcgtcc tgatcctcgc
     2281 ccgaaacctc cagcccccgg tcgatggctt cgacgacata gccggccgcg cggcaggtgt
     2341 cggtgagcgc ggccagcagg gcggcttcct ggtcaggggt caggtcggtc atgggcaaga
     2401 ggtccaggtc gtggtttgtc ggcggcttct agcatggacc gcccgcgccc gtgaggccga
     2461 ggatttcgcg ctggtcagac aacctacttg ccgtccccac atgttagcgc taccaagtgc
     2521 cgacgaacgc gcgccgccga cggtgtcggc gcttcagacg ctcgagtttt ggggagacga
     2581 ccatatgcct gcaggcgcct taattaatat gcatggtacc ttaagatctc gagctcctga
     2641 ctcaggaggt atcgatatgg ataagaaata ctcaataggc ttagctatcg gcacaaatag
     2701 cgtcggatgg gcggtgatca ctgatgaata taaggttccg tctaaaaagt tcaaggttct
     2761 gggaaataca gaccgccaca gtatcaaaaa aaatcttata ggggctcttt tatttgacag
     2821 tggagagaca gcggaagcga ctcgtctcaa acggacagct cgtagaaggt atacacgtcg
     2881 gaagaatcgt atttgttatc tacaggagat tttttcaaat gagatggcga aagtagatga
     2941 tagtttcttt catcgacttg aagagtcttt tttggtggaa gaagacaaga agcatgaacg
     3001 tcatcctatt tttggaaata tagtagatga agttgcttat catgagaaat atccaactat
     3061 ctatcatctg cgaaaaaaat tggtagattc tactgataaa gcggatttgc gcttaatcta
     3121 tttggcctta gcgcatatga ttaagtttcg tggtcatttt ttgattgagg gagatttaaa
     3181 tcctgataat agtgatgtgg acaaactatt tatccagttg gtacaaacct acaatcaatt
     3241 atttgaagaa aaccctatta acgcaagtgg agtagatgct aaagcgattc tttctgcacg
     3301 attgagtaaa tcaagacgat tagaaaatct cattgctcag ctccccggtg agaagaaaaa
     3361 tggcttattt gggaatctca ttgctttgtc attgggtttg acccctaatt ttaaatcaaa
     3421 ttttgatttg gcagaagatg ctaaattaca gctttcaaaa gatacttacg atgatgattt
     3481 agataattta ttggcgcaaa ttggagatca atatgctgat ttgtttttgg cagctaagaa
     3541 tttatcagat gctattttac tttcagatat cctaagagta aatactgaaa taactaaggc
     3601 tcccctatca gcttcaatga ttaaacgcta cgatgaacat catcaagact tgactctttt
     3661 aaaagcttta gttcgacaac aacttccaga aaagtataaa gaaatctttt ttgatcaatc
     3721 aaaaaacgga tatgcaggtt atattgatgg gggagctagc caagaagaat tttataaatt
     3781 tatcaaacca attttagaaa aaatggatgg tactgaggaa ttattggtga aactaaatcg
     3841 tgaagatttg ctgcgcaagc aacggacctt tgacaacggc tctattcccc atcaaattca
     3901 cttgggtgag ctgcatgcta ttttgagaag acaagaagac ttttatccat ttttaaaaga
     3961 caatcgtgag aagattgaaa aaatcttgac ttttcgaatt ccttattatg ttggtccatt
     4021 ggcgcgtggc aatagtcgtt ttgcatggat gactcggaag tctgaagaaa caattacccc
     4081 atggaatttt gaagaagttg tcgataaagg tgcttcagct caatcattta ttgaacgcat
     4141 gacaaacttt gataaaaatc ttccaaatga aaaagtacta ccaaaacata gtttgcttta
     4201 tgagtatttt acggtttata acgaattgac aaaggtcaaa tatgttactg aaggaatgcg
     4261 aaaaccagca tttctttcag gtgaacagaa gaaagccatt gttgatttac tcttcaaaac
     4321 aaatcgaaaa gtaaccgtta agcaattaaa agaagattat ttcaaaaaaa tagaatgttt
     4381 tgatagtgtt gaaatttcag gagttgaaga tagatttaat gcttcattag gtacctacca
     4441 tgatttgcta aaaattatta aagataaaga ttttttggat aatgaagaaa atgaagatat
     4501 cttagaggat attgttttaa cattgacctt atttgaagat agggagatga ttgaggaaag
     4561 acttaaaaca tatgctcacc tctttgatga taaggtgatg aaacagctta aacgtcgccg
     4621 ttatactggt tggggacgtt tgtctcgaaa attgattaat ggtattaggg ataagcaatc
     4681 tggcaaaaca atattagatt ttttgaaatc agatggtttt gccaatcgca attttatgca
     4741 gctgatccat gatgatagtt tgacatttaa agaagacatt caaaaagcac aagtgtctgg
     4801 acaaggcgat agtttacatg aacatattgc aaatttagct ggtagccctg ctattaaaaa
     4861 aggtatttta cagactgtaa aagttgttga tgaattggtc aaagtaatgg ggcggcataa
     4921 gccagaaaat atcgttattg aaatggcacg tgaaaatcag acaactcaaa agggccagaa
     4981 aaattcgcga gagcgtatga aacgaatcga agaaggtatc aaagaattag gaagtcagat
     5041 tcttaaagag catcctgttg aaaatactca attgcaaaat gaaaagctct atctctatta
     5101 tctccaaaat ggaagagaca tgtatgtgga ccaagaatta gatattaatc gtttaagtga
     5161 ttatgatgtc gatgccattg ttccacaaag tttccttaaa gacgattcaa tagacaataa
     5221 ggtcttaacg cgttctgata aaaatcgtgg taaatcggat aacgttccaa gtgaagaagt
     5281 agtcaaaaag atgaaaaact attggagaca acttctaaac gccaagttaa tcactcaacg
     5341 taagtttgat aatttaacga aagctgaacg tggaggtttg agtgaacttg ataaagctgg
     5401 ttttatcaaa cgccaattgg ttgaaactcg ccaaatcact aagcatgtgg cacaaatttt
     5461 ggatagtcgc atgaatacta aatacgatga aaatgataaa cttattcgag aggttaaagt
     5521 gattacctta aaatctaaat tagtttctga cttccgaaaa gatttccaat tctataaagt
     5581 acgtgagatt aacaattacc atcatgccca tgatgcgtat ctaaatgccg tcgttggaac
     5641 tgctttgatt aagaaatatc caaaacttga atcggagttt gtctatggtg attataaagt
     5701 ttatgatgtt cgtaaaatga ttgctaagtc tgagcaagaa ataggcaaag caaccgcaaa
     5761 atatttcttt tactctaata tcatgaactt cttcaaaaca gaaattacac ttgcaaatgg
     5821 agagattcgc aaacgccctc taatcgaaac taatggggaa actggagaaa ttgtctggga
     5881 taaagggcga gattttgcca cagtgcgcaa agtattgtcc atgccccaag tcaatattgt
     5941 caagaaaaca gaagtacaga caggcggatt ctccaaggag tcaattttac caaaaagaaa
     6001 ttcggacaag cttattgctc gtaaaaaaga ctgggatcca aaaaaatatg gtggttttga
     6061 tagtccaacg gtagcttatt cagtcctagt ggttgctaag gtggaaaaag ggaaatcgaa
     6121 gaagttaaaa tccgttaaag agttactagg gatcacaatt atggaaagaa gttcctttga
     6181 aaaaaatccg attgactttt tagaagctaa aggatataag gaagttaaaa aagacttaat
     6241 cattaaacta cctaaatata gtctttttga gttagaaaac ggtcgtaaac ggatgctggc
     6301 tagtgccgga gaattacaaa aaggaaatga gctggctctg ccaagcaaat atgtgaattt
     6361 tttatattta gctagtcatt atgaaaagtt gaagggtagt ccagaagata acgaacaaaa
     6421 acaattgttt gtggagcagc ataagcatta tttagatgag attattgagc aaatcagtga
     6481 attttctaag cgtgttattt tagcagatgc caatttagat aaagttctta gtgcatataa
     6541 caaacataga gacaaaccaa tacgtgaaca agcagaaaat attattcatt tatttacgtt
     6601 gacgaatctt ggagctcccg ctgcttttaa atattttgat acaacaattg atcgtaaacg
     6661 atatacgtct acaaaagaag ttttagatgc cactcttatc catcaatcca tcactggtct
     6721 ttatgaaaca cgcattgatt tgagtcagct aggaggtgac taaatactct ggccattttt
     6781 ctttaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc
     6841 gtgcggccgc tgattagcta agcagaaggc catcctgacg gatgcctttt tcgcttgcta
     6901 gctgcagccc gggggatcca ctagttctag agcggccatt cactggccgt cgttttacaa
     6961 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7021 ttcgccagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7081 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7141 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7201 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7261 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7321 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7381 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7441 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7501 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7561 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7621 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7681 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7741 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7801 ttaagggatt ttggtcatac gaggcccttt cgtcttcaag aattaattca ctggccgtcg
     7861 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7921 atcccccttt cgccagcaga tccgtcccta tcgtttccac gatcagcgat cggctcgttg
     7981 ccctgcgccg ctccaaagcc cgcgacgcag cgccggcagg cagagcaagt agagggcagc
     8041 gcctgcaatc catgcccacc cgttccacgt tgttatagaa gccgcataga tcgccgtgaa
     8101 gaggaggggt ccgacgatcg aggtcaggct ggtgagcgcc gccagtgagc cttgcagctg
     8161 cccctgacgt tcctcatcca cctgcctgga caacattgct tgcagcgccg gcattccgat
     8221 gccacccgaa gcaagcagga ccatgatcgg gaacgccatc catccccgtg tcgcgaaggc
     8281 aagcaggatg tagcctgtgc cgtcggcaat cattccgagc atgagtgccc gcctttcgcc
     8341 gagccgggcg gctacagggc cggtgatcat tgcctgggcg agtgaatgca gaatgccaaa
     8401 tgcggcaagc gaaatgccga tcgtggtcgc gtcccagtga aagcgatcct cgccgaaaat
     8461 gacccaaagc gcggccggca cctgtccgac aagttgcatg atgaagaaga ccgccatcag
     8521 ggcggcgacg acggtcatgc cccgggccca ccggaacgaa gcgagcgggt tgagagcctc
     8581 ccggcgtaac ggccggcgtt cgcctttgtg cgactccggc aaaaggaaac agcccgtcag
     8641 gaaattgagg ccgttcaagg ctgccgcggc gaagaacgga gcgtgggggg agaaaccgcc
     8701 catcagccca ccgagcacag gtcccgcgac catcccgaac ccgaaacagg cgctcatgaa
     8761 gccgaagtgc cgcgcgcgct catcgccatc agtgatatcg gcaatataag cgccggctac
     8821 cgccccagtc gccccggtga tgccggccac gatccgcccg atatagagaa cccaaaggaa
     8881 aggcgctgtc gccatgatgg cgtagtcgac agtggcgccg gccagcgaga cgagcaagat
     8941 tggccgccgc ccgaaacgat ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac
     9001 caacgcatac agcgccagca gaatgccata gtgggcggtg acgtcgttcg agtgaaccag
     9061 atcgcgcagg aggcccggca gcaccggcat aatcaggccg atgccgacag cgtcgagcgc
     9121 gacagtgctc agaattacga tcaggggtat gttgggtttc acgtctggcc tccggaccag
     9181 cctccgctgg tccgattgaa cgcgcggatt ctttatcact gataagttgg tggacatatt
     9241 atgtttatca gtgataaagt gtcaagcatg acaaagttgc agccgaatac agtgatccgt
     9301 gccgccctgg acctgttgaa cgaggtcggc gtagacggtc tgacgacacg caaactggcg
     9361 gaacggttgg gggttcagca gccggcgctt tactggcact tcaggaacaa gcgggcgctg
     9421 ctcgacgcac tggccgaagc catgctggcg gagaatcata cgcattcggt gccgagagcc
     9481 gacgacgact ggcgctcatt tctgatcggg aatgcccgca gcttcaggca ggcgctgctc
     9541 gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac cgggcgcacc gcagatggaa
     9601 acggccgacg cgcagcttcg cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc
     9661 aatgcgctga tgacaatcag ctacttcact gttggggccg tgcttgagga gcaggccggc
     9721 gacagcgatg ccggcgagcg cggcggcacc gttgaacagg ctccgctctc gccgctgttg
     9781 cgggccgcga tagacgcctt cgacgaagcc ggtccggacg cagcgttcga gcagggactc
     9841 gcggtgattg tcgatggatt ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg
     9901 agaaagggtg acgattgatc aggaccgctg ccggagcgca acccactcac tacagcagag
     9961 ccatgtagac aacatcccct ccccctttcc accgcgtcag acgcccgtag cagcccgcta
    10021 cgggcttttt catgccctgc cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg
    10081 cggccttctg gcgctcctgc tgcggcgtcc gctcgtatga tgatatattt ttatcttgtg
    10141 caatgtaaca tcagagattt tgagacacaa cgtggctttc cccccccccc cccaccgcgg
    10201 tggcggccgc cccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
    10261 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
    10321 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
    10381 atatcgtgcg aaaaaggatg gat
//
