LOCUS       sequence-26683-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Maretto S, Cordenonsi M, Dupont S, Braghetta P, Broccoli V, Hassan 
            AB, Volpin D, Bressan GM, Piccolo S
  TITLE     Mapping Wnt/beta-catenin signaling during mouse development and in 
            colorectal tumors.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Mar 18. 100(6):3299-304.
  PUBMED    12626757
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2003 Mar 18. 100(6):3299-304."
COMMENT     Sequence Label: BAT-gal
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             406..426
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(459..481)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgctcccggc cgccatggcg gccgcgggaa ttcgattaag gacctcagaa tcatcaaagg
       61 acctcagaat catcaaagga cctcagaatc atcaaaggac ctcagaatca tcaaaggacc
      121 tcagaatcat caaaggcctc agaatcatca aaggacctca gaatcatcaa aggaccttca
      181 gaatcactag tgaattcgcg gccgcctgca ggtcgacaga ctgacaataa ccacgagcaa
      241 cagtacatca aagtatttat atttttttca tttcccccaa aacacatcct ggacacaccc
      301 tttgtcccct gttgatataa agtctcccag gacagagtgg accaacattt tgggagacag
      361 agataagctt gatatcgaat tcctgcagct gcacctgcca tggctccaaa aaagaagaga
      421 aaggtagaag acccccgcat ggctcgcgat gatcccgtcg ttttacaacg tcgtgactgg
      481 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      541 cgtaatagcg aagaggcccg caccgatcgc ccttcnnnnn agttgcgcan nctgaatggc
      601 gaatggngct ttgcctggtt tccggcaccn naancggtgc cggaaagctg gctggnagtg
      661 cnatcttcct gaggccgata ctgncgtcnt cccctcaaan nnnnnnangc ncggntacna
      721 ngcnccncat ctncaccaac gtgaccta
//
