LOCUS       sequence-266861-       10077 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coryne. glutamicum HA production plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Hoffmann J, Altenbuchner J
  TITLE     Hyaluronic acid production with Corynebacterium glutamicum: effect 
            of media composition on yield and molecular weight.
  JOURNAL   J Appl Microbiol. 2014 Sep;117(3):663-78. doi: 10.1111/jam.12553. 
            Epub 2014 Jun 20.
  PUBMED    24863652
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/jam.12553";
            journalName: "J Appl Microbiol"; date: "2014-09"; volume: "117"; 
            issue: "3"; pages: "663-78"
COMMENT     Sequence Label: pJH181.3
FEATURES             Location/Qualifiers
     source          1..10077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(11..16,17..32,33..39)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    47..63
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     52..74
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     terminator      2472..2543
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2564..2581)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2564..2580)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2793..2879
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2971..2998
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3017..3108
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6312..6331)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6323..7138
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6323..7138
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6396..6415)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7246..7834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7735..7754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7988..8005
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8020..8162
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8088..8107)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8248..8270
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8264..8455)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     8664..8683
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8664..8682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(8698..9780)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9742..9761
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9781..9858)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
ORIGIN
        1 aaatgagctg ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa
       61 caatttcaca caggaaacag aattgaagga gatatacata tgcgtaccct gaaaaacctg
      121 atcaccgttg ttgctttctc tatcttctgg gttctgctga tctacgttaa cgtttacctg
      181 ttcggtgcta aaggttctct gtctatctac ggtttcctgc tgatcgctta cctgctggtt
      241 aaaatgtctc tgtctttctt ctacaaaccg ttcaaaggtc gtgctggtca gtacaaagtt
      301 gctgctatca tcccgtctta caacgaagac gctgaatctc tgctggaaac cctgaaatct
      361 gttcagcagc agacctaccc gctggctgaa atctacgttg ttgacgacgg ttctgctgac
      421 gaaaccggta tcaaacgtat cgaagactac gttcgtgaca ccggtgacct gtcttctaac
      481 gttatcgttc accgttctga aaaaaaccag ggtaaacgtc acgctcaggc ttgggctttc
      541 gaacgttctg acgctgacgt tttcctgacc gttgactctg acacctacat ctacccggac
      601 gctctggaag aactgctgaa aaccttcaac gacccgaccg ttttcgctgc taccggtcac
      661 ctgaacgttc gtaaccgtca gaccaacctg ctgacccgtc tgaccgacat ccgttacgac
      721 aacgctttcg gtgttgaacg tgctgctcag tctgttaccg gtaacatcct ggtttgctct
      781 ggtccgctgt ctgtttaccg tcgtgaagtt gttgttccga acatcgaccg ttacatcaac
      841 cagaccttcc tgggtatccc ggtttctatc ggtgacgacc gttgcctgac caactacgct
      901 accgacctgg gtaaaaccgt ttaccagtct accgctaaat gcatcaccga cgttccggac
      961 aaaatgtcta cctacctgaa acagcagaac cgttggaaca aatctttctt ccgtgaatct
     1021 atcatctctg ttaaaaaaat catgaacaac ccgttcgttg ctctgtggac catcctggaa
     1081 gtttctatgt tcatgatgct ggtttactct gttgttgact tcttcgttgg taacgttcgt
     1141 gaattcgact ggctgcgtgt tctggctttc ctggttatca tcttcatcgt tgctctgtgc
     1201 cgtaacatcc actacatgct gaaacacccg ctgtctttcc tgctgtctcc gttctacggt
     1261 gttctgcacc tgttcgttct gcagccgctg aaactgtact ctctgttcac catccgtaac
     1321 gctgactggg gtacccgtaa aaaactgctg taagctagtc acaggcctag ccttttgtaa
     1381 ggacgtatat gatcaaaaaa tgcttgttcc cggcagccgg ctacggcacg cgcttcttgc
     1441 ctgctaccaa agccatgccc aaggaaatgc tgccggtggt caacaagcca ctgatccagt
     1501 atggcgttga agaagcattg gatgcgggtc tgaacgagat ctccatcgtt accgggcgcg
     1561 gcaagcgcgc cctggaagac cacttcgaca tcagctacga gctggaaaac cagatcaagg
     1621 gcaccgacaa ggaaaaatac ctggtcggta ttcgtcgtct gctcaacgag tgctcgttct
     1681 cgtacacgcg ccagaccgaa atgaagggcc tcggccacgc cattctcact ggccgcccac
     1741 tgatcggtga cgagccgttc gcggtggtgc tggcggacga cctgtgcgtc aacccggaag
     1801 gtgacggcgt gctgactcag atggtcaagc tgtacaaaca gtaccgctgc tcgatcgtcg
     1861 ccatccagga agtcgacccg caggaaacca acaagtatgg tgtgatcgcc ggtgagatga
     1921 tccgcgacga catcttccgt gtgaccaaca tggtcgaaaa accggccccg gaagacgcgc
     1981 cttcgaacct ggcgatcatc ggccgttaca tcctgacccc ggatatcttc gacatcatcg
     2041 ccaacaccaa gccaggcaaa ggcggtgaaa tacagatcac cgacgccctg ctgcagcagg
     2101 cgaaggatgg ctgcgtgatt gcctataagt tcaagggcaa gcgtttcgac tgcggtggcg
     2161 ccgaaggcta catcgacgcg accaacttct gcttcgagaa ctactacaag actggcaagg
     2221 cttactgatc tagaaagctt tttttatcgc tagctgtaca agtaagcttg gctgttttgg
     2281 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     2341 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     2401 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     2461 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2521 gttgtttgtc ggtgaacgct ctcctggtac cgagctcgaa ttcactggcc gtcgttttac
     2581 agccaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
     2641 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
     2701 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
     2761 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     2821 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     2881 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
     2941 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
     3001 cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3061 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3121 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3181 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3241 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3301 ccaatgatga gcacttttga tccccctgcg gcgtcgctga tcgccctcgc gacgttgtgc
     3361 gggtggcttg tccctgaggg cgctgcgaca gatagctaaa aatctgcgtc aggatcgccg
     3421 tagagcgcgc gtcgcgtcga ttggaggctt cccctttggt tgacggtctt caatcgctct
     3481 acggcgatcc tgacgctttt ttgttgcgta ccgtcgatcg ttttatttct gtcgatcccg
     3541 aaaaagtttt tgccttttgt aaaaaacttc tcggtcgccc cgcaaatttt cgattccaga
     3601 ttttttaaaa accaagccag aaatacgaca caccgtttgc agataatctg tctttcggaa
     3661 aaatcaagtg cgatacaaaa tttttagcac ccctgagctg cgcaaagtcc cgcttcgtga
     3721 aaattttcgt gccgcgtgat tttccgccaa aaactttaac gaacgttcgt tataatggtg
     3781 tcatgacctt cacgacgaag taccaaaatt ggcccgaatc atcagctatg gatctctctg
     3841 atgtcgcgct ggagtccgac gcgctcgatg ctgccgtcga tttaaaaacg gtgatcggat
     3901 ttttccgagc tctcgatacg acggacgcgc cagcatcacg agactgggcc agtgccgcga
     3961 gcgacctaga aactctcgtg gcggatcttg aggagctggc tgacgagctg cgtgctcggc
     4021 agcgccagga ggacgcacag tagtggagga tcgaatcagt tgcgcctact gcggtggcct
     4081 gattcctccc cggcctgacc cgcgaggacg gcgcgcaaaa tattgctcag atgcgtgtcg
     4141 tgccgcagcc agccgcgagc gcgccaacaa acgccacgcc gaggagctgg aggcggctag
     4201 gtcgcaaatg gcgctggaag tgcgtccccc gagcgaaatt ttggccatgg tcgtcacaga
     4261 gctggaagcg gcagcgagaa ttatccgcga tcgtggcgcg gtgcccgcag gcatgacaaa
     4321 catcgtaaat gccgcgtttc gtgtggccgt ggccgcccag gacgtgtcag cgccgccacc
     4381 acctgcaccg aatcggcagc agcgtcgcgc gtcgaaaaag cgcacaggcg gcaagaagcg
     4441 ataagctgca cgaatacctg aaaaatgttg aacgccccgt gagcggtaac tcacagggcg
     4501 tcggctaacc cccagtccaa acctgggaga aagcgctcaa aaatgactct agcggattca
     4561 cgagacattg acacaccggc ctggaaattt tccgctgatc tgttcgacac ccatcccgag
     4621 ctcgcgctgc gatcacgtgg ctggacgagc gaagaccgcc gcgaattcct cgctcacctg
     4681 ggcagagaaa atttccaggg cagcaagacc cgcgacttcg ccagcgcttg gatcaaagac
     4741 ccggacacgg gagaaacaca gccgaagtta taccgagttg gttcaaaatc gcttgcccgg
     4801 tgccagtatg ttgctctgac gcacgcgcag cacgcagccg tgcttgtcct ggacattgat
     4861 gtgccgagcc accaggccgg cgggaaaatc gagcacgtaa accccgaggt ctacgcgatt
     4921 ttggagcgct gggcacgcct ggaaaaagcg ccagcttgga tcggcgtgaa tccactgagc
     4981 gggaaatgcc agctcatctg gctcattgat ccggtgtatg ccgcagcagg catgagcagc
     5041 ccgaatatgc gcctgctggc tgcaacgacc gaggaaatga cccgcgtttt cggcgctgac
     5101 caggcttttt cacataggct gagccggtgg ccactgcacg tctccgacga tcccaccgcg
     5161 taccgctggc atgcccagca caatcgcgtg gatcgcctag ctgatcttat ggaggttgct
     5221 cgcatgatct caggcacaga aaaacctaaa aaacgctatg agcaggagtt ttctagcgga
     5281 cgggcacgta tcgaagcggc aagaaaagcc actgcggaag caaaagcact tgccacgctt
     5341 gaagcaagcc tgccgagcgc cgctgaagcg tctggagagc tgatcgacgg cgtccgtgtc
     5401 ctctggactg ctccagggcg tgccgcccgt gatgagacgg cttttcgcca cgctttgact
     5461 gtgggatacc agttaaaagc ggctggtgag cgcctaaaag acaccaagat catcgacgcc
     5521 tacgagcgtg cctacaccgt cgctcaggcg gtcggagcag acggccgtga gcctgatctg
     5581 ccgccgatgc gtgaccgcca gacgatggcg cgacgtgtgc gcggctacgt cgctaaaggc
     5641 cagccagtcg tccctgctcg tcagacagag acgcagagca gccgagggcg aaaagctctg
     5701 gccactatgg gaagacgtgg cggtaaaaag gccgcagaac gctggaaaga cccaaacagt
     5761 gagtacgccc gagcacagcg agaaaaacta gctaagtcca gtcaacgaca agctaggaaa
     5821 gctaaaggaa atcgcttgac cattgcaggt tggtttatga ctgttgaggg agagactggc
     5881 tcgtggccga caatcaatga agctatgtct gaatttagcg tgtcacgtca gaccgtgaat
     5941 agagcactta agtctgcggg cattgaactt ccacgaggac gccgtaaagc ttcccagtaa
     6001 atgtgccatc tcgtaggcag aaaacggttc cccccgtagg ggtctctctc ttggcctcct
     6061 ttctaggtcg ggctgattgc tcttgaagct ctctaggggg gctcacacca taggcagata
     6121 acggttcccc accggctcac ctcgtaagcg cacaaggact gctcccaaag atctacgcgc
     6181 cttcaactca gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg
     6241 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     6301 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctccaggcc
     6361 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     6421 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
     6481 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     6541 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     6601 tgcatggtta ctcaccactg cgatcccagg gaaaacagca ttccaggtat tagaagaata
     6661 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     6721 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     6781 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     6841 gcctgttgaa caagtctgga aagaaatgca taagctattg ccattctcac cggattcagt
     6901 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     6961 ttgtattgat gttgggcgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     7021 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     7081 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     7141 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgg
     7201 ctttgttgca cgtttaaacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7261 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7321 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7381 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7441 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7501 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7561 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7621 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7681 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7741 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7801 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7861 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7921 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7981 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     8041 tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct
     8101 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     8161 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     8221 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     8281 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
     8341 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
     8401 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     8461 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
     8521 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
     8581 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     8641 atgcctgcag gtcgactcta gattaatacg actcactata gggcaattgc gttgcgctca
     8701 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8761 gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac
     8821 gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac
     8881 gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
     8941 tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc
     9001 ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc
     9061 agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact
     9121 ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca
     9181 gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg
     9241 ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa
     9301 aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt
     9361 gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc
     9421 actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg
     9481 ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc
     9541 gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga
     9601 ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc
     9661 cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga
     9721 aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac
     9781 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
     9841 gcaccattcg atggtgtcaa cgtaaatgcc gcttcgcctt cgcgcgcgaa ttgcaagctg
     9901 atccgggctt atcgactgca cggtgcacca atgcttctgg cgtcaggcag ccatcggaag
     9961 ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact
    10021 cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctg
//
