LOCUS       sequence-266893-        4652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Macrophage specific label.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Oehlers SH, Cronan MR, Scott NR, Thomas MI, Okuda KS, Walton EM, 
            Beerman RW, Crosier PS, Tobin DM
  TITLE     Interception of host angiogenic signalling limits mycobacterial 
            growth.
  JOURNAL   Nature. 2015 Jan 29;517(7536):612-5. doi: 10.1038/nature13967. Epub 
            2014 Nov 24.
  PUBMED    25470057
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13967"; journalName: "Nature"; date: "2015-01-29- 
            29"; volume: "517"; issue: "7536"; pages: "612-5"
COMMENT     Sequence Label: p5E mpeg1
FEATURES             Location/Qualifiers
     source          1..4652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(91..118)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(210..296)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     345..367
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     359..376
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     360..376
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    416..511
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        513..531
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2449..2465)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2449..2465)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2502..2520)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2521..2644
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2717..2735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2740..2756)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2740..2756)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2858..2877)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2869..3678
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2936..2955)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3825..4413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4314..4333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4567..4584
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
       61 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
      121 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
      181 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
      241 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
      301 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
      361 taaaacgacg gccagtctta agctcgggcc cgagttaacg ctaccatgga gctccaaata
      421 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
      481 tttataatgc caactttgta tagaaaagtt ggtaatacga ctcactatag ggcgaattgg
      541 gtaccgggcc ccccctcgag ttgttggagc acatctgaca tctgaaatta tttgaaagca
      601 gcagaaatgt ttttacagct attacaaaat ttacttaaca agaatccttt aaaatatgac
      661 aatttattgc caataattta cagtaagttt tgcagtactt taatattaca aattatacaa
      721 ctgtgctggc acacaaaaaa taacttaatc cagcacaaca acaacaacaa caactacaaa
      781 aagcatattt ccttttgctt tttcttgttc actcaacttt tggaaacatc aactgcactg
      841 acctaaaatt atacagtatg tcagtaatac aaaaacactt agttaggtta acataagatt
      901 attagttttc tggagaggtg aactactctg taagaaacct ttttaagttg tgccaactaa
      961 acatttttat cagcagaact acaatgcttg atgagtgaac aaaaaaacaa acaaacctta
     1021 tttccttttg ttttttcttg tgtacttaac atttttttca cctttatttg aaacgacagt
     1081 agggagtatg gacaggaaag tgttgggaga ggagagggat gagcatagaa ccaggaggta
     1141 ggaatcaaac tcaggtcact atgaacaccg gagtgccagg tgtcaacaca ctaaccacta
     1201 caccatcaac tccaacattt acttgaaaac atcagttgag tgaacaaaaa actcttgtgg
     1261 aaattggtac tgagaaataa tacattatca caacaaacaa aacagagaca aatgttaaca
     1321 aaacaattat tagaaacaga attagaaacc ggacaggagg taaaacaata atgataaaca
     1381 agacaaggat caaaacatgg caggacaaag acagtttttt gttcactcaa cctcagatat
     1441 tttaggtcag tgcaactgat gtttacaaag gttgtatgca tgtcaacacc aatggtgtag
     1501 tgattagtgt gttgacacaa ggcactccag tgttcacact gatgctggtt tgattcccac
     1561 atcgatgtcc tttgctaatc ctgcctctct ccccatgctt tcctgtctat actctctact
     1621 gttgtgtcaa ttaaaggtga aaaaagttaa gcacaaaaga aaaatctaag gatataagga
     1681 ttatgttttt ttttgttcac tcaaaatcag gcattccagt tctgcagaca aaaaatgttc
     1741 agttggcaca acttaaaaag ttttgttaca gagtagttca cctctccaga aaactaataa
     1801 tcttatgtta acccaactaa atgtttttac tgaaaaataa ttgaatgtca gtgcagctga
     1861 tgttttttta aaagtggaga gaacaaaaaa agtgaaagga aataaggatt cattgttgta
     1921 ccgacttatt tttttgaaga aaattctgct atagggcttt catgatcccc tatgctcaca
     1981 gcagttttag gagatgctta taataaagag gatttgaaaa acctgctaat cttgtacatt
     2041 tctaaaacaa gaagccaacc aatttcaatt cctcctcttt caaagccaat ggaaaattta
     2101 aaacgcacag tctcttgcgt catcaaaacc attctgaaga ctcatcaaaa tccttcgtag
     2161 ctttaaatat aaatctatta ttgaacagat catacttcac aaaaaatata aatatatttg
     2221 attcattcac ttcatgaaat tgtccttcaa cttaacgttt gaacgcaagg taccaaaaca
     2281 caaagaggaa cctcatagtc tcatgaccct tcccccacgg atttgaaact gagaagtggt
     2341 tttattgagg aactcagcaa aatctataat caccatattt gattgaaaat cacattagtg
     2401 caggagacag caaaacaaag cttgatatcg aattcctgca gcccggggga tccactagtt
     2461 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     2521 caagtttgta caaaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2581 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atgcagtcac
     2641 tatgaaccaa ctacttagat ggtattagtg acctgtagaa ttcgagctct agagctgcag
     2701 ggcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     2761 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     2821 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     2881 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2941 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     3001 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     3061 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     3121 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     3181 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     3241 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     3301 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     3361 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     3421 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     3481 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     3541 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     3601 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     3661 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     3721 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3781 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3841 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3901 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3961 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4021 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4081 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4141 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4201 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4261 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4321 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4381 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4441 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4501 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4561 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4621 cccgcgcgtt ggccgattca ttaatgcagc tg
//
