LOCUS       sequence-266904-        3978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red flourescent tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3978)
  TITLE     Tobin lab unpublished plamids
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pME tdTomato No stop (for 5' tagging)
FEATURES             Location/Qualifiers
     source          1..3978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(70..97)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(189..275)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     324..346
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     338..355
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     339..355
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    371..470
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             476..1903
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(1905..2004)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2021..2040)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2022..2040)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2045..2061)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2045..2061)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2163..2182)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2174..2983
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2241..2260)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3130..3718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3619..3638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3872..3889
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt
       61 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt
      121 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct
      181 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
      241 gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
      301 gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa
      361 gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
      421 tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggt
      481 gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc
      541 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      601 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
      661 gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga
      721 ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      781 cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa
      841 ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat
      901 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
      961 ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta
     1021 catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat
     1081 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca
     1141 ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc
     1201 ctccgaggac aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga
     1261 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     1321 gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga
     1381 catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat
     1441 ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     1501 cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat
     1561 ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa
     1621 gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg
     1681 cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac
     1741 catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct
     1801 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg
     1861 ccgccaccac ctgttcctgt acggcatgga cgagctgtac aaggacccag ctttcttgta
     1921 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     1981 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2041 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2101 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2161 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2221 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2281 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2341 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2401 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2461 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     2521 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2581 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2641 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2701 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2761 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     2821 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     2881 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     2941 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3001 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3061 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3241 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3841 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3901 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     3961 cgacaggttt cccgactg
//
