LOCUS       sequence-266934-        1749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express tTALE-EmGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Shin E, Kim W, Lee S, Bae J, Kim S, Ko W, Seo HS, Lim S, Lee HS, Jo 
            K
  TITLE     Truncated TALE-FP as DNA Staining Dye in a High-salt Buffer.
  JOURNAL   Sci Rep. 2019 Nov 20;9(1):17197. doi: 10.1038/s41598-019-53722-0.
  PUBMED    31748571
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-11-20"; volume: "9(1):17197. doi"; pages: " 
            10.1038/s41598-019-53722-0"
COMMENT     Sequence Label: pET-tTALE-EmGFP-15b
FEATURES             Location/Qualifiers
     source          1..1749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1033..1035,1036..1038,1039..1749)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1033..1035,1036..1038,1039..1749)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1078..1099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1339..1358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1686..1707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatctacgca cgctcggcta cagccagcag caacaggaga agatcaaacc gaaggttcgt
       61 tcgacagtgg cgcagcacca cgaggcactg gtcggccatg ggtttacaca cgcgcacatc
      121 gttgcgctca gccaacaccc ggcagcgtta gggaccgtcg ctgtcaagta tcaggacatg
      181 atcgcagcgt tgccagaggc gacacacgaa gcgatcgttg gcgtcggcaa acagtggtcc
      241 ggcgcacgcg ctctggaggc cttgctcacg gtggcgggag agttgagagg tccaccgtta
      301 cagttggaca caggccaact tctcaagatt gcaaaacgtg gcggcgtgac cgcagtggag
      361 gcagtgcatg catggcgcaa tgcactgacg ggtgcccccc tgaacctgac tccagcacag
      421 gtggtggcta ttgcctccaa taatggggga aaacaggctc tcgaaacagt tcaaagactt
      481 ctgcctgtct tgtgccaaga tcatggactt acacccgcac aagtcgttgc aatcgctagc
      541 aatggcggtg ggaagcaagc cctggagaca gttcaaaggc tccttccagt cctgtgtcag
      601 gcacacgggc ttacccctga tcaagtggtc gcaattgcct cacatgacgg cgggaaacag
      661 gcattggaaa ctgtgcagcg cctgctcccc gttctttgcc aggatcatgg gttgacacct
      721 gcacaagttg ttgcaattgc aagcaacggc ggcggaaaac aggcactgga aactgtgcaa
      781 agactcctgc cagttttgtg ccaggctcat ggcctgactc cagaccaggt cgttgctatt
      841 gccagcaata acggaggtaa gcaagcattg gagactgttc agcggctcct ccctgtcctc
      901 tgtcaggccc acgggctgac tcctgcacag gtggtggcca tcgcttcaaa cggggggggg
      961 aagcaagccc tcgaaactgt tcaacggttg ttgcctgttt tgtgtcagga tcatgggggc
     1021 ggctctggcg gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1081 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1141 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1201 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1261 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1321 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1381 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1441 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1501 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1561 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1621 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1681 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1741 ctgtacaag
//
