LOCUS       sequence-266935-        1740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express tTALE-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Shin E, Kim W, Lee S, Bae J, Kim S, Ko W, Seo HS, Lim S, Lee HS, Jo 
            K
  TITLE     Truncated TALE-FP as DNA Staining Dye in a High-salt Buffer.
  JOURNAL   Sci Rep. 2019 Nov 20;9(1):17197. doi: 10.1038/s41598-019-53722-0.
  PUBMED    31748571
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-11-20"; volume: "9(1):17197. doi"; pages: " 
            10.1038/s41598-019-53722-0"
COMMENT     Sequence Label: pET-TALE-mCherry-15b
FEATURES             Location/Qualifiers
     source          1..1740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1033..1740
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDQMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1178..1196)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1446..1465
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1640..1663
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gatctacgca cgctcggcta cagccagcag caacaggaga agatcaaacc gaaggttcgt
       61 tcgacagtgg cgcagcacca cgaggcactg gtcggccatg ggtttacaca cgcgcacatc
      121 gttgcgctca gccaacaccc ggcagcgtta gggaccgtcg ctgtcaagta tcaggacatg
      181 atcgcagcgt tgccagaggc gacacacgaa gcgatcgttg gcgtcggcaa acagtggtcc
      241 ggcgcacgcg ctctggaggc cttgctcacg gtggcgggag agttgagagg tccaccgtta
      301 cagttggaca caggccaact tctcaagatt gcaaaacgtg gcggcgtgac cgcagtggag
      361 gcagtgcatg catggcgcaa tgcactgacg ggtgcccccc tgaacctgac tccagcacag
      421 gtggtggcta ttgcctccaa taatggggga aaacaggctc tcgaaacagt tcaaagactt
      481 ctgcctgtct tgtgccaaga tcatggactt acacccgcac aagtcgttgc aatcgctagc
      541 aatggcggtg ggaagcaagc cctggagaca gttcaaaggc tccttccagt cctgtgtcag
      601 gcacacgggc ttacccctga tcaagtggtc gcaattgcct cacatgacgg cgggaaacag
      661 gcattggaaa ctgtgcagcg cctgctcccc gttctttgcc aggatcatgg gttgacacct
      721 gcacaagttg ttgcaattgc aagcaacggc ggcggaaaac aggcactgga aactgtgcaa
      781 agactcctgc cagttttgtg ccaggctcat ggcctgactc cagaccaggt cgttgctatt
      841 gccagcaata acggaggtaa gcaagcattg gagactgttc agcggctcct ccctgtcctc
      901 tgtcaggccc acgggctgac tcctgcacag gtggtggcca tcgcttcaaa cggggggggg
      961 aagcaagccc tcgaaactgt tcaacggttg ttgcctgttt tgtgtcagga tcatgggggc
     1021 ggctctggcg gcatggtgag caagggcgag gaggaccaga tggccatcat caaggagttc
     1081 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     1141 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     1201 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     1261 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     1321 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     1381 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1441 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1501 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1561 cactacgacg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1621 gcctacaacg tgaacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg
     1681 gaacagtacg agcgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
//
