LOCUS       sequence-266942-         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-221-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-221-5p
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(99..123)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(347..366)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    412..467
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(466..485)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 cgacaaagag acatacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc
       61 tagcaaactg gggcacaaac ttaattgaga attctaccac ccagctttct tgtacaaagt
      121 ggtacgtctc aagaccagag gtctctgctt aaattaggaa atctacattg tatgccaggt
      181 aaatctacat tgtatgccag gtaaatctac attgtatgcc aggtaaatct acattgtatg
      241 ccaggtcaac cagaagagac ctgtgcttca ctcctcaggt gcaggctgcc tatcagaagg
      301 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
      361 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
      421 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
      481 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
      541 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
      601 aacagccccc tgctgtccat
//
