LOCUS       sequence-266958-        9709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Gal4AD-Prey hybrid protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, 
            Beltran-Sastre V, Serrano L, Maurer SP
  TITLE     rec-YnH enables simultaneous many-by-many detection of direct 
            protein-protein and protein-RNA interactions.
  JOURNAL   Nat Commun. 2018 Sep 14;9(1):3747. doi: 10.1038/s41467-018-06128-x.
  PUBMED    30217970
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-09-14"; volume: "9(1):3747. doi"; pages: " 
            10.1038/s41467-018-06128-x"
COMMENT     Sequence Label: pAW
FEATURES             Location/Qualifiers
     source          1..9709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1475
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1557..1898
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1904..1923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1904..1922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1929..1938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1941..1967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2025..2149
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2174..2179,2180..2197,2198..2204)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2258..2917
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2324..2343)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3259..3564
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3443..3462
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3605..3729)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4137..4324
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4192..4216)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4441..5535)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5536..5941)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5983..5999)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5983..5999)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5996..6018)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6007..6023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6031..6037,6038..6055,6056..6061))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6076..6097
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6152..6185
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6263..6296
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6365..6382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6536..7124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6616..6635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7295..8086,8087..8155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7918..7937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8156..8260)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8328..8346
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8384..8406)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8506..8525
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8542..9706
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg
     1981 aggccagtga attccacccg ggtgggcatc gatacgggat ctccacaagt ttgtacaaaa
     2041 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     2101 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
     2161 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
     2221 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     2281 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     2341 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     2401 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     2461 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     2521 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     2581 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     2641 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     2701 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     2761 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     2821 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     2881 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa
     2941 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga
     3001 tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca
     3061 gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg
     3121 taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa
     3181 atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg
     3241 agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat
     3301 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc
     3361 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     3421 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     3481 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     3541 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
     3601 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     3661 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta
     3721 caaagtggta agtaactaag atccatcgag ctcgagctgc agatgaatcg tagatactga
     3781 aaaaccccgc aagttcactt caactgtgca tcgtgcacca tctcaatttc tttcatttat
     3841 acatcgtttt gccttctttt atgtaactat actcctctaa gtttcaatct tggccatgta
     3901 acctctgatc tatagaattt tttaaatgac tagaattaat gcccatcttt tttttggacc
     3961 taaattcttc atgaaaatat attacgaggg cttattcaga agctttggac ttcttcgcca
     4021 gaggtttggt caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg
     4081 aaaagatgga aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga
     4141 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     4201 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     4261 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta
     4321 ccggccggtc gaaattcccc taccctatga acatattcca ttttgtaatt tcgtgtcgtt
     4381 tctattatga atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt
     4441 ttaagcaagg attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt
     4501 ggaaccacct aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc
     4561 aatggcctta ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa
     4621 gatagtggcg atagggttga ccttattctt tggcaaatct ggagcagaac cgtggcatgg
     4681 ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa
     4741 caaacccaag gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt
     4801 gctggtgatt ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga
     4861 atcaatcaat tgatgttgaa ccttcaatgt aggaaattcg ttcttgatgg tttcctccac
     4921 agtttttctc cataatcttg aagaggccaa aacattagct ttatccaagg accaaatagg
     4981 caatggtggc tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc
     5041 tggaacggtg tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt
     5101 accaaagtaa atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa
     5161 ttgtggcttg attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa
     5221 gttggcgtac aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact
     5281 acctgtaccc catttaggac cacccacagc acctaacaaa acggcatcag ccttcttgga
     5341 ggcttccagc gcctcatctg gaagtgggac acctgtagct tcgatagcag caccaccaat
     5401 taaatgattt tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac
     5461 cttaatggct tcggctgtga tttcttgacc aacgtggtca cctggcaaaa cgacgatctt
     5521 cttaggggca gacattagaa tggtatatcc ttgaaatata tatatatatt gctgaaatgt
     5581 aaaaggtaag aaaagttaga aagtaagacg attgctaacc acctattgga aaaaacaata
     5641 ggtccttaaa taatattgtc aacttcaagt attgtgatgc aagcatttag tcatgaacgc
     5701 ttctctattc tatatgaaaa gccggttccg gcgctctcac ctttcctttt tctcccaatt
     5761 tttcagttga aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa aatttccagt
     5821 catcgaattt gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt gaggaaaaaa
     5881 ataatggttg ctaagagatt cgaactcttg catcttacga tacctgagta ttcccacagt
     5941 tgggggatct cgactctagc tagaggatca attcgtaatc atgtcatagc tgtttcctgt
     6001 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6061 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6121 tttccagtcg ggaaacctgt cgtgccagct gataacttcg tataatgtat gctatacgaa
     6181 gttattaggt ctgaagagga gtttacgtcc agccaagcta gcttggctgc aggtcgagcg
     6241 gccgcgatcc ggaaccctta atataacttc gtataatgta tgctatacga agttatcagc
     6301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8281 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8341 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     8401 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     8461 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     8521 ttgtactgag agtgcaccat aacgcattta agcataaaca cgcactatgc cgttcttctc
     8581 atgtatatat atatacaggc aacacgcaga tataggtgcg acgtgaacag tgagctgtat
     8641 gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt tgaagttcct
     8701 attccgaagt tcctattctc tagctagaaa gtataggaac ttcagagcgc ttttgaaaac
     8761 caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct
     8821 actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat
     8881 atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat
     8941 ctaaactcga cctctacatt ttttatgttt atctctagta ttactcttta gacaaaaaaa
     9001 ttgtagtaag aactattcat agagtgaatc gaaaacaata cgaaaatgta aacatttcct
     9061 atacgtagta tatagagaca aaatagaaga aaccgttcat aattttctga ccaatgaaga
     9121 atcatcaacg ctatcacttt ctgttcacaa agtatgcgca atccacatcg gtatagaata
     9181 taatcgggga tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa
     9241 cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa tagacaccaa agtagccttc
     9301 ttctaacctt aacggaccta cagtgcaaaa agttatcaag agactgcatt atagagcgca
     9361 caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc tcgggatgca
     9421 tttttgtaga acaaaaaaga agtatagatt ctttgttggt aaaatagcgc tctcgcgttg
     9481 catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg
     9541 ttgcattttt gttttacaaa aatgaagcac agattcttcg ttggtaaaat agcgctttcg
     9601 cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc
     9661 tcgcgttgca tttttgttct acaaaatgaa gcacagatgc ttcgttgct
//
