LOCUS       sequence-266964-        9865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Gal4AD-Prey hybrid protein. Homology regions for 
            recombination with pBKWH, pBSWHhc or pMS22H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, 
            Beltran-Sastre V, Serrano L, Maurer SP
  TITLE     rec-YnH enables simultaneous many-by-many detection of direct 
            protein-protein and protein-RNA interactions.
  JOURNAL   Nat Commun. 2018 Sep 14;9(1):3747. doi: 10.1038/s41467-018-06128-x.
  PUBMED    30217970
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-09-14"; volume: "9(1):3747. doi"; pages: " 
            10.1038/s41467-018-06128-x"
COMMENT     Sequence Label: pAWH
FEATURES             Location/Qualifiers
     source          1..9865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1474
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1520..1540
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1556..1897
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1903..1922
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1903..1921
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1928..1937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1940..1966
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2024..2148
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2173..2178,2179..2196,2197..2203)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2257..2916
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2323..2342)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3258..3563
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3442..3461
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3604..3728)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4176..4363
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4231..4255)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4480..5574)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5575..5980)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(6022..6038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6022..6038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6035..6057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6046..6062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6070..6076,6077..6094,6095..6100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6115..6136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6191..6224
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6302..6335
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6521..6538)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6692..7280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6772..6791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7451..8242,8243..8311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8074..8093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8312..8416)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8484..8502
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8540..8562)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8662..8681
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8698..9862
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc ttttttttct tttctctctc ccccgttgtt gtctcaccat atccgcaatg
     1141 acaaaaaaat gatggaagac actaaaggaa aaaattaacg acaaagacag caccaacaga
     1201 tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac acgaaacttt ttccttcctt
     1261 cattcacgca cactactctc taatgagcaa cggtatacgg ccttccttcc agttacttga
     1321 atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta taaatagacc tgcaattatt
     1381 aatcttttgt ttcctcgtca ttgttctcgt tccctttctt ccttgtttct ttttctgcac
     1441 aatatttcaa gctataccaa gcatacaatc aactccaagc tttgcaaaga tggataaagc
     1501 ggaattaatt cccgagcctc caaaaaagaa gagaaaggtc gaattgggta ccgccgccaa
     1561 ttttaatcaa agtgggaata ttgctgatag ctcattgtcc ttcactttca ctaacagtag
     1621 caacggtccg aacctcataa caactcaaac aaattctcaa gcgctttcac aaccaattgc
     1681 ctcctctaac gttcatgata acttcatgaa taatgaaatc acggctagta aaattgatga
     1741 tggtaataat tcaaaaccac tgtcacctgg ttggacggac caaactgcgt ataacgcgtt
     1801 tggaatcact acagggatgt ttaataccac tacaatggat gatgtatata actatctatt
     1861 cgatgatgaa gataccccac caaacccaaa aaaagagatc tttaatacga ctcactatag
     1921 ggcgagcgcc gccatggagt acccatacga cgtaccagat tacgctcata tggccatgga
     1981 ggccagtgaa ttccacccgg gtgggcatcg atacgggatc tccacaagtt tgtacaaaaa
     2041 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     2101 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta
     2161 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     2221 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     2281 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     2341 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     2401 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     2461 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2521 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2581 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     2641 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2701 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2761 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2821 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2881 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2941 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     3001 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     3061 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     3121 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     3181 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     3241 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     3301 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     3361 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     3421 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     3481 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     3541 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     3601 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     3661 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3721 aaagtggtaa gtaactaaga tccatcgagc tcgagctgca gatgaatcgt agatactgaa
     3781 aaaccccgca agttcacttc aactgtgcat cgtgcaccat ctcaatttct ttcatttata
     3841 catcgttttg ccttctttta tgtaactata ctcctctaag tttcaatctt ggccatgtaa
     3901 cctctgataa cagggtaatt ggcaaacagc tattatgggt attatgggtg tttaaactag
     3961 ggataacagg gtaatacatt caagcgcatt agtaatgagt gaatataact ataacggtcc
     4021 taacgagggc ttattcagaa gctttggact tcttcgccag aggtttggtc aagtctccaa
     4081 tcaaggttgt cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa
     4141 tcgttggtag atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt
     4201 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     4261 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     4321 aggtatagca tgaggtcgct cttattgacc acacctctac cggccggtcg aaattcccct
     4381 accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat
     4441 aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac
     4501 ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt
     4561 tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg
     4621 caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac
     4681 cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt
     4741 gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat
     4801 aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt
     4861 taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac
     4921 cttcaatgta ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga
     4981 agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag
     5041 ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact
     5101 atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac
     5161 taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa
     5221 gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc
     5281 tttacggatt tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc
     5341 acccacagca cctaacaaaa cggcatcagc cttcttggag gcttccagcg cctcatctgg
     5401 aagtgggaca cctgtagctt cgatagcagc accaccaatt aaatgatttt cgaaatcgaa
     5461 cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat
     5521 ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat
     5581 ggtatatcct tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa
     5641 agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca
     5701 acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag
     5761 ccggttccgg cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat
     5821 gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg
     5881 atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc
     5941 gaactcttgc atcttacgat acctgagtat tcccacagtt gggggatctc gactctagct
     6001 agaggatcaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6061 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6181 gtgccagctg ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag
     6241 tttacgtcca gccaagctag cttggctgca ggtcgagcgg ccgcgatccg gaacccttaa
     6301 tataacttcg tataatgtat gctatacgaa gttatcagct gcattaatga atcggccaac
     6361 gcgataacag ggtaatacat tcaagcgcat tagtaatgag tgaatataac tataacggtc
     6421 ctaaggtagc gaaagaatca ccagttacat atatggtgat agtaactata acggtcctaa
     6481 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6541 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6601 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6661 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6721 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6781 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6841 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6901 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6961 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7021 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7081 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7141 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7201 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7261 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7321 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7381 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7441 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7501 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7561 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7621 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7681 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7741 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7801 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7861 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7921 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7981 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8041 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8101 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8161 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8221 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8281 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8341 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8401 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     8461 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     8521 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     8581 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     8641 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccataacg
     8701 catttaagca taaacacgca ctatgccgtt cttctcatgt atatatatat acaggcaaca
     8761 cgcagatata ggtgcgacgt gaacagtgag ctgtatgtgc gcagctcgcg ttgcattttc
     8821 ggaagcgctc gttttcggaa acgctttgaa gttcctattc cgaagttcct attctctagc
     8881 tagaaagtat aggaacttca gagcgctttt gaaaaccaaa agcgctctga agacgcactt
     8941 tcaaaaaacc aaaaacgcac cggactgtaa cgagctacta aaatattgcg aataccgctt
     9001 ccacaaacat tgctcaaaag tatctctttg ctatatatct ctgtgctata tccctatata
     9061 acctacccat ccacctttcg ctccttgaac ttgcatctaa actcgacctc tacatttttt
     9121 atgtttatct ctagtattac tctttagaca aaaaaattgt agtaagaact attcatagag
     9181 tgaatcgaaa acaatacgaa aatgtaaaca tttcctatac gtagtatata gagacaaaat
     9241 agaagaaacc gttcataatt ttctgaccaa tgaagaatca tcaacgctat cactttctgt
     9301 tcacaaagta tgcgcaatcc acatcggtat agaatataat cggggatgcc tttatcttga
     9361 aaaaatgcac ccgcagcttc gctagtaatc agtaaacgcg ggaagtggag tcaggctttt
     9421 tttatggaag agaaaataga caccaaagta gccttcttct aaccttaacg gacctacagt
     9481 gcaaaaagtt atcaagagac tgcattatag agcgcacaaa ggagaaaaaa agtaatctaa
     9541 gatgctttgt tagaaaaata gcgctctcgg gatgcatttt tgtagaacaa aaaagaagta
     9601 tagattcttt gttggtaaaa tagcgctctc gcgttgcatt tctgttctgt aaaaatgcag
     9661 ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttt tacaaaaatg
     9721 aagcacagat tcttcgttgg taaaatagcg ctttcgcgtt gcatttctgt tctgtaaaaa
     9781 tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttctacaa
     9841 aatgaagcac agatgcttcg ttgct
//
