LOCUS       sequence-266969-       10879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Positive control when used with pMS22H-IRE. Expression of Gal4AD-IRP
            hybrid protein. Homology regions for recombination with pMS22H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, 
            Beltran-Sastre V, Serrano L, Maurer SP
  TITLE     rec-YnH enables simultaneous many-by-many detection of direct 
            protein-protein and protein-RNA interactions.
  JOURNAL   Nat Commun. 2018 Sep 14;9(1):3747. doi: 10.1038/s41467-018-06128-x.
  PUBMED    30217970
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-09-14"; volume: "9(1):3747. doi"; pages: " 
            10.1038/s41467-018-06128-x"
COMMENT     Sequence Label: pAWH-IRP
FEATURES             Location/Qualifiers
     source          1..10879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1474
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1520..1540
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1556..1897
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1903..1922
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1903..1921
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1928..1937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1940..1966
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2024..2048
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4718..4742)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      5190..5377
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5245..5269)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5494..6588)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6589..6994)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(7036..7052)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7036..7052)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7049..7071)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7060..7076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7084..7090,7091..7108,7109..7114))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7129..7150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    7205..7238
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7316..7349
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7535..7552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7706..8294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7786..7805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8465..9256,9257..9325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9088..9107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9326..9430)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9498..9516
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9554..9576)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9676..9695
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      9712..10876
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc ttttttttct tttctctctc ccccgttgtt gtctcaccat atccgcaatg
     1141 acaaaaaaat gatggaagac actaaaggaa aaaattaacg acaaagacag caccaacaga
     1201 tgtcgttgtt ccagagctga tgaggggtat ctcgaagcac acgaaacttt ttccttcctt
     1261 cattcacgca cactactctc taatgagcaa cggtatacgg ccttccttcc agttacttga
     1321 atttgaaata aaaaaaagtt tgctgtcttg ctatcaagta taaatagacc tgcaattatt
     1381 aatcttttgt ttcctcgtca ttgttctcgt tccctttctt ccttgtttct ttttctgcac
     1441 aatatttcaa gctataccaa gcatacaatc aactccaagc tttgcaaaga tggataaagc
     1501 ggaattaatt cccgagcctc caaaaaagaa gagaaaggtc gaattgggta ccgccgccaa
     1561 ttttaatcaa agtgggaata ttgctgatag ctcattgtcc ttcactttca ctaacagtag
     1621 caacggtccg aacctcataa caactcaaac aaattctcaa gcgctttcac aaccaattgc
     1681 ctcctctaac gttcatgata acttcatgaa taatgaaatc acggctagta aaattgatga
     1741 tggtaataat tcaaaaccac tgtcacctgg ttggacggac caaactgcgt ataacgcgtt
     1801 tggaatcact acagggatgt ttaataccac tacaatggat gatgtatata actatctatt
     1861 cgatgatgaa gataccccac caaacccaaa aaaagagatc tttaatacga ctcactatag
     1921 ggcgagcgcc gccatggagt acccatacga cgtaccagat tacgctcata tggccatgga
     1981 ggccagtgaa ttccacccgg gtgggcatcg atacgggatc tccacaagtt tgtacaaaaa
     2041 agcaggctta atgagcaacc catttgcata ccttgctgag ccgttagatc ctgcacaacc
     2101 aggaaagaag ttcttcaatt tgaataaatt agactattca agatatggac gcttaccgtt
     2161 ttcaatcaga gttctcctgg aggcagccgt tcggaactgt gataagtttt tggtgaagaa
     2221 agaagacatt gaaaatatcc tgaactggaa tgtcacgcag catatgaaca tagaagtgcc
     2281 attcaaaccc gcccgtgtca tcctgcagga cttcacgggt gtaccatctg tggttgactt
     2341 tgctgcaatg cgtgatgctg tgaaaaaatt agggggtgat ccagagaaaa taaaccctat
     2401 ctgtcctgtt gatcttgtaa tcgatcactc catccaggtt gatttcaaca gaagggctga
     2461 cagcttacag aagaatcaag acctagaatt tgaaagaaat agagaacgat ttgaattttt
     2521 aaagtggggt tccaaggctt ttcgaaacat gcggattatc cctcccggct caggaatcat
     2581 ccaccaagtg aatctggaat acttggccag ggtagtgttt gatcaggatg gatactacta
     2641 ccccgacagc ctcgtgggca ccgattcgca cacgaccatg attgacggtt tgggcgttct
     2701 tggctggggt gtgggtggca ttgaagcaga agctgtcatg ctgggtcagc ctatcagtat
     2761 ggtgcttccc caggtgattg gctacaggct gatgggaaag ccccaccctc tggtaacatc
     2821 cactgacatc gtgctcacca ttaccaagca cctccgccag gttggggtcg tgggcaaatt
     2881 tgttgagttc ttcgggccag gagtagccca gttgtccatt gctgacagag ctacaatcgc
     2941 caacatgtgt ccagaatacg gagcaactgc taccttcttc ccagttgatg aagtcagtat
     3001 caagtacttg gtgcagacag gtcgggatga aagcaaagtg aagcagatta gaaagtatct
     3061 tcaggctgta gggatgttcc gagattacag tgacccctct caagacccag acttcaccca
     3121 ggttgtggaa ttagatttga agaccgtagt gccttgctgc agtggaccca agaggcctca
     3181 ggacaaagtt gctgtgtctg atatgaaaaa ggactttgag agctgccttg gagccaagca
     3241 aggatttaaa ggattccagg ttgctccaga ccatcataat gaccataaga cgtttatcta
     3301 taatgatagt gaattcaccc tttctcatgg atctgtggta attgctgcca ttactagctg
     3361 cacaaacacc agtaatccat ctgtgatgtt aggagcagga ctgttagcga agaaagccgt
     3421 ggatgctggc ttgaacgtga agccctacgt gaagaccagc ctgtcccctg gaagtggtgt
     3481 ggtcacctac tacctgcgag agagtggagt catgccttac ttgtctcagc tggggtttga
     3541 tgtggtgggc tatggctgca tgacctgcat cggcaacagt ggacccttac ctgagcctgt
     3601 ggtagaagcc atcacacagg gagaccttgt agctgtcgga gtactctctg ggaacagaaa
     3661 ttttgaaggt cgagttcatc ccaacacccg ggccaactat ttggcctctc ctcccttagt
     3721 aatagcatat gcaattgctg ggaccatcag gatcgacttt gagaaagagc cattggggac
     3781 gaacgcaaag gggcagcagg tgtttctgag agatatctgg ccaactcgag aggagatcca
     3841 ggcagtggag cggcagtacg tcatccctgg gatgttcacg gaggtctacc agaaaataga
     3901 gactgtgaat gcaagctgga atgccttagc agccccatca gataagctgt atctctggaa
     3961 tcccaaatcc acatacatta agtcaccacc attctttgaa aacctgactt tggacctgca
     4021 gccccctaaa tctatagtgg atgcctatgt gctgttaaat ttgggagatt cagtaacgac
     4081 ggaccacatc tctccagctg gaaatattgc aagaaatagc cctgctgctc gctacttaac
     4141 caacagaggg ctaactccac gagaattcaa ctcctatggc tcccgccggg ggaatgatgc
     4201 catcatggca cgggggacgt tcgccaacat tcgcttgtta aacagatttt tgaacaagca
     4261 ggcaccacag actatacatc tgccttctgg agaaaccctt gatgtgtttg atgcagctga
     4321 gcggtaccag caggaaggcc atcccctgat cgtccttgct ggcaaggagt atggctcagg
     4381 cagctcccga gactgggcag ccaagggccc tttcctgctg ggaatcaaag ctgtcctggc
     4441 tgagagttac gagcgcattc accgcagtaa cctggttgga atgggggtga tcccccttga
     4501 atacctccct ggtgagaacg cagactccct gggactcaca ggacgggaac gatacaccat
     4561 catcattcca gaaaacctca caccacggat gcatgtccag gtcaagctgg acaccgggaa
     4621 gaccttccag gccgtcatac ggtttgacac tgacgtggag ctcacttatt tccacaatgg
     4681 cggcatactc aactacatga tccgcaagat ggcatagacc cagctttctt gtacaaagtg
     4741 gtaagtaact aagatccatc gagctcgagc tgcagatgaa tcgtagatac tgaaaaaccc
     4801 cgcaagttca cttcaactgt gcatcgtgca ccatctcaat ttctttcatt tatacatcgt
     4861 tttgccttct tttatgtaac tatactcctc taagtttcaa tcttggccat gtaacctctg
     4921 ataacagggt aattggcaaa cagctattat gggtattatg ggtgtttaaa ctagggataa
     4981 cagggtaata cattcaagcg cattagtaat gagtgaatat aactataacg gtcctaacga
     5041 gggcttattc agaagctttg gacttcttcg ccagaggttt ggtcaagtct ccaatcaagg
     5101 ttgtcggctt gtctaccttg ccagaaattt acgaaaagat ggaaaagggt caaatcgttg
     5161 gtagatacgt tgttgacact tctaaataag cgaatttctt atgatttatg atttttatta
     5221 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa
     5281 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat
     5341 agcatgaggt cgctcttatt gaccacacct ctaccggccg gtcgaaattc ccctacccta
     5401 tgaacatatt ccattttgta atttcgtgtc gtttctatta tgaatttcat ttataaagtt
     5461 tatgtacaaa tatcataaaa aaagagaatc tttttaagca aggattttct taacttcttc
     5521 ggcgacagca tcaccgactt cggtggtact gttggaacca cctaaatcac cagttctgat
     5581 acctgcatcc aaaacctttt taactgcatc ttcaatggcc ttaccttctt caggcaagtt
     5641 caatgacaat ttcaacatca ttgcagcaga caagatagtg gcgatagggt tgaccttatt
     5701 ctttggcaaa tctggagcag aaccgtggca tggttcgtac aaaccaaatg cggtgttctt
     5761 gtctggcaaa gaggccaagg acgcagatgg caacaaaccc aaggaacctg ggataacgga
     5821 ggcttcatcg gagatgatat caccaaacat gttgctggtg attataatac catttaggtg
     5881 ggttgggttc ttaactagga tcatggcggc agaatcaatc aattgatgtt gaaccttcaa
     5941 tgtaggaaat tcgttcttga tggtttcctc cacagttttt ctccataatc ttgaagaggc
     6001 caaaacatta gctttatcca aggaccaaat aggcaatggt ggctcatgtt gtagggccat
     6061 gaaagcggcc attcttgtga ttctttgcac ttctggaacg gtgtattgtt cactatccca
     6121 agcgacacca tcaccatcgt cttcctttct cttaccaaag taaatacctc ccactaattc
     6181 tctgacaaca acgaagtcag tacctttagc aaattgtggc ttgattggag ataagtctaa
     6241 aagagagtcg gatgcaaagt tacatggtct taagttggcg tacaattgaa gttctttacg
     6301 gatttttagt aaaccttgtt caggtctaac actacctgta ccccatttag gaccacccac
     6361 agcacctaac aaaacggcat cagccttctt ggaggcttcc agcgcctcat ctggaagtgg
     6421 gacacctgta gcttcgatag cagcaccacc aattaaatga ttttcgaaat cgaacttgac
     6481 attggaacga acatcagaaa tagctttaag aaccttaatg gcttcggctg tgatttcttg
     6541 accaacgtgg tcacctggca aaacgacgat cttcttaggg gcagacatta gaatggtata
     6601 tccttgaaat atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag
     6661 acgattgcta accacctatt ggaaaaaaca ataggtcctt aaataatatt gtcaacttca
     6721 agtattgtga tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt
     6781 ccggcgctct cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgtca
     6841 ggcgacctct gaaattaaca aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg
     6901 cccctgtgtg ttctcgttat gttgaggaaa aaaataatgg ttgctaagag attcgaactc
     6961 ttgcatctta cgatacctga gtattcccac agttggggga tctcgactct agctagagga
     7021 tcaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7081 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7141 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7201 gctgataact tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg
     7261 tccagccaag ctagcttggc tgcaggtcga gcggccgcga tccggaaccc ttaatataac
     7321 ttcgtataat gtatgctata cgaagttatc agctgcatta atgaatcggc caacgcgata
     7381 acagggtaat acattcaagc gcattagtaa tgagtgaata taactataac ggtcctaagg
     7441 tagcgaaaga atcaccagtt acatatatgg tgatagtaac tataacggtc ctaacgggga
     7501 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8761 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9061 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     9481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     9541 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     9601 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     9661 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat aacgcattta
     9721 agcataaaca cgcactatgc cgttcttctc atgtatatat atatacaggc aacacgcaga
     9781 tataggtgcg acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc
     9841 gctcgttttc ggaaacgctt tgaagttcct attccgaagt tcctattctc tagctagaaa
     9901 gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa
     9961 aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa
    10021 acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac
    10081 ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt
    10141 atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc
    10201 gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga
    10261 aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa
    10321 agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat
    10381 gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg
    10441 gaagagaaaa tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa
    10501 agttatcaag agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct
    10561 ttgttagaaa aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt
    10621 ctttgttggt aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga
    10681 ttctttgttt gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac
    10741 agattcttcg ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc
    10801 tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa
    10861 gcacagatgc ttcgttgct
//
