LOCUS       sequence-26697-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  piggyBac empty vector with Gateway cassette, tet inducible promoter,
            and linked reporter (IRES-bgeo).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hamalainen
            R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A
  TITLE     piggyBac transposition reprograms fibroblasts to induced pluripotent
            stem cells.
  JOURNAL   Nature. 2009 Apr 9;458(7239):766-70. doi: 10.1038/nature07863
  PUBMED    19252478
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2009-04-9- 9"; volume: "458"; issue: "7239"; pages:
            "766-70"
COMMENT     Sequence Label: PB-TET
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   37..99
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    471..489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    508..526
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    545..563
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    582..600
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    619..637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    656..674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    693..711
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ggctgaataa taaaaaaatt agaaactatt atttaaccct agaaagataa tcatattgtg
       61 acgtacgtta aagataatca tgcgtaaaat tgacgcatgt gttttatcgg tctgtatatc
      121 gaggtttatt tattaatttg aatagatatt aagttttatt atatttacac ttacatacta
      181 ataataaatt caacaaacaa tttatttatg tttatttatt tattaaaaaa aaacaaaaac
      241 tcaaaatttc ttctataaag taacaaaact tttaaacatt ctctctttta caaaaataaa
      301 cttattttgt actttaaaaa cagtcatgtt gtattataaa ataagtaatt agcttaacct
      361 atacataata gaaacaaatt atacttatta gtcagtcaga aacaactttg gcacatatca
      421 atattatgct ctcgttaatt aactcgaggg atccaagctg gccgcacgtc tccctatcag
      481 tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga tagagaaggt acgtctagaa
      541 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
      601 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
      661 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
      721 gtctcgagct ccctatcagt gata
//
