LOCUS       sequence-266981-        9025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Gal4BD-Bait hybrid protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, 
            Beltran-Sastre V, Serrano L, Maurer SP
  TITLE     rec-YnH enables simultaneous many-by-many detection of direct 
            protein-protein and protein-RNA interactions.
  JOURNAL   Nat Commun. 2018 Sep 14;9(1):3747. doi: 10.1038/s41467-018-06128-x.
  PUBMED    30217970
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-09-14"; volume: "9(1):3747. doi"; pages: " 
            10.1038/s41467-018-06128-x"
COMMENT     Sequence Label: pBW
FEATURES             Location/Qualifiers
     source          1..9025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1317..1441
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1466..1471,1472..1489,1490..1496)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1550..2209
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1616..1635)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2551..2856
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2735..2754
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2897..3021)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3060..3107
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3134..3321
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3189..3213)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3345..3361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3345..3361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3358..3380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3369..3385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3393..3399,3400..3417,3418..3423))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3438..3459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3576..3593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3747..4335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3827..3846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4664..4711
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4736..4755
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4943..5737)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4943..5737)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5054..5073)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5664..5683
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5738..5842)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5869..7211
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6832..6879)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7252..7270
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7308..7330)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7430..7449
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7470..7751
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7752..8426
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8529..8984)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8666..8687)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8878..8897
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcccgggg atctccacaa
     1321 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     1381 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     1441 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
     1501 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     1561 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     1621 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     1681 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     1741 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
     1801 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     1861 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     1921 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     1981 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     2041 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     2101 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     2161 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc
     2221 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
     2281 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt
     2341 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
     2401 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
     2461 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
     2521 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga
     2581 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
     2641 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     2701 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     2761 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     2821 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
     2881 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
     2941 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     3001 agctttcttg tacaaagtgg taagtaacta agatccgtcg acctgcagcg gccgcataac
     3061 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgcgc gcttgcagcc
     3121 aagctaattc cgggcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
     3181 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
     3241 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct
     3301 tattgaccac acctctaccg gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     3361 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3421 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3481 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3541 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3601 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3661 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4201 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct
     4501 atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
     4561 gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg
     4621 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
     4681 ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc
     4741 tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc
     4801 ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca
     4861 tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc
     4921 atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     4981 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     5041 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     5101 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     5161 agcaggcatc gccatgagtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc
     5221 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     5281 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     5341 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     5401 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     5461 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     5521 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     5581 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     5641 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     5701 ccggagaacc tgcgtgcaat ccatcttgtt caatcatact cttccttttt caatattatt
     5761 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5821 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa cgaagcatct
     5881 gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
     5941 ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga
     6001 atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa
     6061 agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac
     6121 aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta
     6181 acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat
     6241 aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct
     6301 cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg
     6361 gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca
     6421 tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac
     6481 ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt
     6541 tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag
     6601 agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg
     6661 atgggtaggt tatataggga tatagcacag agatatatag caaagagata cttttgagca
     6721 atgtttgtgg aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt
     6781 ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct
     6841 atactttcta gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc
     6901 gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata
     6961 tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct
     7021 taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt
     7081 gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt
     7141 tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt
     7201 gatattggat catactaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7261 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7321 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7381 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     7441 cagattgtac tgagagtgca ccatagatca acgacattac tatatatata atataggaag
     7501 catttaatag aacagcatcg taatatatgt gtactttgca gttatgacgc cagatggcag
     7561 tagtggaaga tattctttat tgaaaaatag cttgtcacct tacgtacaat cttgatccgg
     7621 agcttttctt tttttgccga ttaagaatta attcggtcga aaaaagaaaa ggagagggcc
     7681 aagagggagg gcattggtga ctattgagca cgtgagtata cgtgattaag cacacaaagg
     7741 cagcttggag tatgtctgtt attaatttca caggtagttc tggtccattg gtgaaagttt
     7801 gcggcttgca gagcacagag gccgcagaat gtgctctaga ttccgatgct gacttgctgg
     7861 gtattatatg tgtgcccaat agaaagagaa caattgaccc ggttattgca aggaaaattt
     7921 caagtcttgt aaaagcatat aaaaatagtt caggcactcc gaaatacttg gttggcgtgt
     7981 ttcgtaatca acctaaggag gatgttttgg ctctggtcaa tgattacggc attgatatcg
     8041 tccaactgca tggagatgag tcgtggcaag aataccaaga gttcctcggt ttgccagtta
     8101 ttaaaagact cgtatttcca aaagactgca acatactact cagtgcagct tcacagaaac
     8161 ctcattcgtt tattcccttg tttgattcag aagcaggtgg gacaggtgaa cttttggatt
     8221 ggaactcgat ttctgactgg gttggaaggc aagagagccc cgaaagctta cattttatgt
     8281 tagctggtgg actgacgcca gaaaatgttg gtgatgcgct tagattaaat ggcgttattg
     8341 gtgttgatgt aagcggaggt gtggagacaa atggtgtaaa agactctaac aaaatagcaa
     8401 atttcgtcaa aaatgctaag aaataggtta ttactgagta gtatttattt aagtattgtt
     8461 tgtgcacttg ccgatctatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     8521 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     8581 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     8641 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     8701 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     8761 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     8821 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     8881 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     8941 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     9001 ctgcgcaact gttgggaagg gcgat
//
