LOCUS       sequence-267007-        2104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for TEF1 sgRNA, Part 234(promoter,coding region, 
            terminator).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM764
FEATURES             Location/Qualifiers
     source          1..2104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        300..375
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(472..506)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(577..1236)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1151..1170
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1418..2006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1907..1926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagtcggtct ccaacgatgt gcttcagtat tacatttttt gccttcaacg ccttgattgt
       61 tctatttttg ctaataataa atctatttca tcggactaaa agtccattag ttgtaagcgg
      121 atttagctca gttgggagag cgccagactg aagaaaaact tcggtcaagt catctggagg
      181 tcctgtgttc gatccacaga attcgcagat ggccggcatg gtcccagcct cctcgctggc
      241 gccggctggg caacaccttc gggtggcgaa tgggactttt tgatatttaa gttaataaag
      301 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      361 gcaccgagtc ggtgcttttt ttattttttg tcactattgt tatgtaaaat gccacctctg
      421 acagtatgga acgcaaactt ctgtctagtg gatagctgtg agaccagacc aataaaaaac
      481 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
      541 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
      601 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      661 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      721 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
      781 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
      841 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
      901 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
      961 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     1021 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     1081 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     1141 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     1201 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     1261 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     1321 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
     1381 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1441 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1501 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1561 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1621 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1681 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1741 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1801 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1861 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1921 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1981 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2041 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2101 accg
//
