LOCUS       sequence-267025-        3045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dominate Negative zebrafish e-cadherin label.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Cronan MR, Beerman RW, Rosenberg AF, Saelens JW, Johnson MG, Oehlers
            SH, Sisk DM, Jurcic Smith KL, Medvitz NA, Miller SE, Trinh LA, 
            Fraser SE, Madden JF, Turner J, Stout JE, Lee S, Tobin DM
  TITLE     Macrophage Epithelial Reprogramming Underlies Mycobacterial 
            Granuloma Formation and Promotes Infection.
  JOURNAL   Immunity. 2016 Oct 18;45(4):861-876. doi: 
            10.1016/j.immuni.2016.09.014.
  PUBMED    27760340
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2016.09"; journalName: "Immunity"; date: 
            "2016-10-18- 18"; volume: "45"; issue: "4"; pages: "861-876"
COMMENT     Sequence Label: pME DN-E-cadherin
FEATURES             Location/Qualifiers
     source          1..3045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     327..349
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     341..358
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     342..358
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    374..473
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             479..520
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     protein_bind    complement(975..1074)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1091..1110)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1092..1110)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1115..1131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1115..1131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1233..1252)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1244..2053
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1311..1330)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2200..2788
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2689..2708
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2942..2959
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
      361 taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
      421 aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagcag gctggaccat
      481 gggaagctct aagagcaagc ctaaagaccc aagccagaga agacggagga aaagcaactc
      541 caaaaaagaa ccgctccttc cagaagatga tgtacgggac aacatctact attatgatga
      601 ggaaggcggt ggagaagatg atcaggactt tgatttgagt gtcctgcaca gaggcctgga
      661 caacagacca gaagtgttca ggaatgatgt ggcgcccact ttcatgcctg cacctcagta
      721 cagacctcga ccggccaacc ctgaggagat cggcacattc attgatgata acctgaaggc
      781 tgcagataac gaccccacag ctcctcccta cgactctctg ctggtgtttg actatgaagg
      841 aggaggatca gacgcaggtt cactcagctc cctcaacact tccagctcag gaaacgacca
      901 agactatgac ttcctcaatg agtggggccc acgcttcaag aagctagcgg acatgtatgg
      961 aggaggagag gataacccag ctttcttgta caaagttggc attataagaa agcattgctt
     1021 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     1081 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     1141 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
     1201 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     1261 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     1321 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     1381 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     1441 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     1501 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     1561 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     1621 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     1681 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     1741 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     1801 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     1861 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     1921 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     1981 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     2041 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     2101 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     2161 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2221 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2281 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2341 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2401 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2461 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2521 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2581 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2641 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2701 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2761 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2821 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2881 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2941 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     3001 gcgttggccg attcattaat gcagctggca cgacaggttt cccga
//
