LOCUS       sequence-267026-       10514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of recombinant proteins in plants thanks to efficient
            translational efficiency. Empty vector, clone ORF using Bsa1 sites. 
            Test UTR combinations by swapping 5' and 3' UTRs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10514)
  AUTHORS   Peyret H, Brown JKM, Lomonossoff GP
  TITLE     Improving plant transient expression through the rational design of 
            synthetic 5' and 3' untranslated regions.
  JOURNAL   Plant Methods. 2019 Sep 18;15:108. doi: 10.1186/s13007-019-0494-9. 
            eCollection 2019.
  PUBMED    31548848
REFERENCE   2  (bases 1 to 10514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2019-09-18"; volume: "15:108. doi"; pages: " 
            10.1186/s13007-019-0494-9. eCollection 2019"
COMMENT     Sequence Label: pHRE
FEATURES             Location/Qualifiers
     source          1..10514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      446..1077
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(1167..2315)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2614..3408)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     complement(3998..4015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4249..4268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4700..4724
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     4863..4882
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5007..5029
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5021..5038
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5022..5038
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5508..5553
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      5849..6101
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        6249..6594
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    8067..8243
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        8809..8988
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        8841..9024
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             9045..9839
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9099..9118)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9709..9728
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      10225..10477
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
       61 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
      121 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
      181 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
      241 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
      301 ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat
      361 tcatttctcg cttaaccgtg acagagtaga caggctgtct cgcggccgag gggcgcagcc
      421 cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc
      481 cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata
      541 aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa
      601 cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc
      661 cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta
      721 gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc
      781 tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac
      841 gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc
      901 cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat
      961 ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt
     1021 gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga
     1081 aaggcgctcg gtcttgcctt gctcgtcggt gatgtacact agtcgctggc tgctgaaccc
     1141 ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc
     1201 caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg
     1261 cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg
     1321 agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac
     1381 agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg
     1441 atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg
     1501 aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc
     1561 gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc
     1621 gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg
     1681 gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc
     1741 tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc
     1801 agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg
     1861 tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt
     1921 tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt
     1981 tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg
     2041 atggtcatcg acttcgccaa acctgccgcc tcctgttcaa gacgacgcga acgctccacg
     2101 gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg
     2161 gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg
     2221 acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct
     2281 tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg
     2341 actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc
     2401 agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg
     2461 tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg
     2521 ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc
     2581 ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca gtaaaatata
     2641 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     2701 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     2761 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     2821 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     2881 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
     2941 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     3001 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     3061 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     3121 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     3181 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     3241 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     3301 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
     3361 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
     3421 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     3481 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     3541 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatgg
     3601 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
     3661 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
     3721 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
     3781 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
     3841 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
     3901 cacttaaaaa actcaggccg cagtcggtaa ctatgcggtg tgaaataccg cacagatgcg
     3961 taaggagaaa ataccgcatc aggcgctatt ccgcttcctc gctcactgac tcgctgcgct
     4021 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4081 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4141 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4201 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4261 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4321 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4381 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4441 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4501 acttatcgcc actggcagca ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc
     4561 tgcgcggaaa tggacgggcc cccggcgcca gatctgggga accctgtggt tggcatgcac
     4621 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
     4681 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttgt
     4741 gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     4801 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     4861 gaagggaaga aagcgaaagg agcgggcgcc attcaggctg cgcaactgtt gggaagggcg
     4921 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     4981 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     5041 attgttagag ggagctctga tcactgagca tcgttgaaga tgcctctgcc gacagtggtc
     5101 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     5161 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     5221 actatccttc ttaattaagg aaacctcctc ggattccatt gcccagctat ctgtcacttt
     5281 attgagaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     5341 aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
     5401 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     5461 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     5521 tctatataag gaagttcatt tcatttggag aggtttaaga gacgcaacca caacgctcta
     5581 acgcaatcaa tctacattat attaaacgtc tctaaaaaga gacctaggta actgactagg
     5641 gtctctcgcc gaagagcgca tcggatctaa taataaactt aggcaataaa tttcgacatc
     5701 ataataaagc cacgtatcta catcaacaca agattagtat tttcaaaact gttttcagca
     5761 gaacacatat tttcattttt aacgtaattt tcatttagcg ttagtacagt caccttcaca
     5821 gaacgtttgg agaagtgagg gctcttccga tcgttcaaac atttggcaat aaagtttctt
     5881 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     5941 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     6001 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     6061 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cggcgcgcca gcttggcgta
     6121 atcatggtca tagctgttgc gattaataat tccacgcgga tcgcattgag aacccggaat
     6181 atcgcgaaac aggtaccccc ctactccaaa aatgtcaaag atacagtctc agaagaccaa
     6241 agggctattg agacttttca acaaagggta atttcgggaa acctcctcgg attccattgc
     6301 ccagctatct gtcacttcat cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc
     6361 catcattgcg ataaaggaaa ggctatcatt caagatgcct ctgccgacag tggtcccaaa
     6421 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     6481 aagcaagtgg attgatgtga catctccact gacgtaaggg atgacgcaca atcccactat
     6541 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag gacagcccaa
     6601 gcttcgactc tagaggatcc ccttaaatcg atatgagcac cgccaagatg agcgctggtg
     6661 agttctctaa gacctacggc accaaggata gccggtctgt gaatgattgc tacgccatct
     6721 acagcggcaa cggcatcaac ttcatcaacc tgttcatgca caccaacacc ggcatcaaga
     6781 gcgccttctc tattaacgac cttggccgga acgaggacat taagcttcat gaggccgaga
     6841 tcatcaacag cttgcacccg tactcattct tcgacaagtt cggcctggat atcatcctgt
     6901 gcaaccacgt gatggacatc atcgtgacta agcctggcct taagaacacc ggatgcaagt
     6961 tccagatgca caaccagatc ttcaacccga atgaggatat tctggctaag acccctggca
     7021 tcatcagcga agagaacttc tacgacctga gcaagatcaa gcctaagggc atgacacctt
     7081 tcgactggta catcaacgag tgcaagaagt acgacttcta catcagcgac aacggcgaca
     7141 tctctctgga ttacggtttc cctgtgatgg gcaagaccac ctcttattgg agagagaaca
     7201 tcccgcgaga gaagatcatc tccgttaagc agaagtgcct gccgaacacc tctgctctta
     7261 ccaacaggat tctgagcctt tctaccgtga gggctatcca gattgcttct gagctggctt
     7321 ctgaaaagac cgtggttctt gcttctaggc agaggctgga tctggacatc aagtctcagt
     7381 accggatctc attccctggt gttcaagatg agggcgcttt cactaggact ttctgcatcc
     7441 ctatggacaa gaccgctagg atcgtttgct tctacgctaa gacctccgtg gacgtgtcaa
     7501 atgagaggac caccttgacc atcaagatcg tcaacaagac cgtcgagagc aactacatgg
     7561 gtcctgtgcc taaggatcac atgtactgcg ataagagcat cggcgctagg attggtcttg
     7621 tggatattgt gaggggcgac ccgaactaca atcagatgat tgctcgggaa atgatcagcg
     7681 tccacaccaa ctttgctctg aggctttctg aggctctgaa gaagccggtc atcgttttca
     7741 agatgtacga gaaagagctg aacttcgaga cttacgacct gtccggtcgg tctttgtctt
     7801 accagaagga cagcagcggc aatatctact tcctgtctag gacccttgag atcctgccga
     7861 agtctctttc taccctgacc tacctgaaga acatctctcc tgcctgctgg aaagaatcca
     7921 tcagcatgca gcatttctac atcggggagc aagaagaggg accttctggt tctaatctgg
     7981 ccgagtctga agagaaggtg cagactgctt aggaattcgg tacgctgaaa tcaccagtct
     8041 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
     8101 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
     8161 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     8221 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
     8281 ccagacaccg gacgactaaa cctggagccc agacgccgtt cgaagctaga agtaccgctt
     8341 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
     8401 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
     8461 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
     8521 atactatacg ctaagggaat gcttgtattt ataccctata ccccctaata accccttatc
     8581 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     8641 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag ttcttaactc
     8701 taaagataaa agatggcgcg tggccggcca tgagcggaga attaagggag tcacgttatg
     8761 acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt
     8821 tgaaggagcc actcagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa
     8881 accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt
     8941 caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt
     9001 cactctcaat ccaaataatc tgcaccggat ctggatcgtt tcgcatgatt gaacaagatg
     9061 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     9121 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     9181 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     9241 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     9301 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     9361 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     9421 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     9481 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagaacat caggggctcg
     9541 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgat gatctcgtcg
     9601 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     9661 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     9721 gtgatattgc tgaagaactt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     9781 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     9841 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     9901 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     9961 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacg ggatctctgc
    10021 gaaacaggcg gtcgaaggtg ccgatatcat tacgacagca acggccgaca agcacaacgc
    10081 cacgatcctg agcgacaata tgatcgcggc gtccacatca acggcgtcgg cggcgactgc
    10141 ccaggcaaga ccgagatgca ccgcgatatc ttgctgcgtt cggatatttt cgtggagttc
    10201 ccgccacaga cccggatgat ccccgatcgt tcaaacattt ggcaataaag tttcttaaga
    10261 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
    10321 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
    10381 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
    10441 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg ggccggccct cactggtgaa
    10501 aagaaaaacc accc
//
