LOCUS       sequence-267038-        2104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for ERG11 sgRNA, Part 234(promoter,coding region, 
            terminator).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM755
FEATURES             Location/Qualifiers
     source          1..2104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        297..372
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(469..503)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(574..1233)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1148..1167
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1415..2003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1904..1923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcca acgatgtgct tcagtattac attttttgcc ttcaacgcct tgattgttct
       61 atttttgcta ataataaatc tatttcatcg gactaaaagt ccattagttg taagcggatt
      121 tagctcagtt gggagagcgc cagactgaag aaaaacttcg gtcaagtcat ctggaggtcc
      181 tgtgttcgat ccacagaatt cgcagatggc cggcatggtc ccagcctcct cgctggcgcc
      241 ggctgggcaa caccttcggg tggcgaatgg gacttttata taatgaatac acatgggttt
      301 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
      361 ccgagtcggt gcttttttta ttttttgtca ctattgttat gtaaaatgcc acctctgaca
      421 gtatggaacg caaacttctg tctagtggat agctgtgaga ccagaccaat aaaaaacgcc
      481 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      541 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
      601 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      661 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      721 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      781 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
      841 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
      901 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
      961 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1021 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1081 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1141 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1201 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1261 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1321 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1381 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1441 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1501 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1561 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1621 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1681 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1741 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1801 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1861 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1921 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1981 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2041 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2101 gtag
//
