LOCUS       sequence-267046-       11053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SpyTag003-Talin rod-mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, 
            Hytonen VP, Howarth M
  TITLE     Approaching infinite affinity through engineering of peptide-protein
            interaction.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 
            10.1073/pnas.1909653116.
  PUBMED    31822621
REFERENCE   2  (bases 1 to 11053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Dec 10. pii: 1909653116. doi: 
            10.1073/pnas.1909653116."
COMMENT     Sequence Label: SpyTag003-Talin rod-mCherry
FEATURES             Location/Qualifiers
     source          1..11053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      242..251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             254..301
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
     CDS             6626..7336
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6771..6789)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7039..7058
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7477..7598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7514..7533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7568..7587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7605..8060)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7742..7763)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7954..7973
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8087..8191
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8193..8550
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8193..8384
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8216..8236)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8401..8536
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8585..9379
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8585..9379
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8639..8658)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9249..9268
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9567..9586)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9611..9658
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9987..10575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10476..10495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        10750..11053
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tgccaccatg ggccgtggcg tgcctcatat cgtgatggtg gacgcctaca agcgttacaa
      301 gcagcagtac aaccgagtgg ggaaagtgga gcacggctct gtggctctgc cagccatcat
      361 gcgctctgga gcctctggtc ctgagaattt ccaggtgggg agcatgccac ctgcccagca
      421 gcagatcacc agtggccaga tgcaccgagg acacatgcca cctctgactt cagcccagca
      481 ggcgctcact ggaaccatta actccagcat gcaggctgtg caggctgccc aggccactct
      541 ggatgacttt gagactctac cccctcttgg ccaggatgct gcctccaaag cttggcgtaa
      601 gaacaagatg gatgaatcaa agcatgagat ccactcccag gtagatgcca tcacagctgg
      661 cactgcctct gtggtaaacc tgacagcagg agatcctgca gagacagact atacagcagt
      721 gggctgtgca gtcaccacca tctcttccaa cctgacggag atgtcccgtg gggtgaagct
      781 attggctgcc ctgctggagg atgaaggcgg caatggccgg ccccttctgc aagcagcaaa
      841 gggccttgca ggggctgtgt cggaactgct tcgcagtgct cagcccgcca gcgctgagcc
      901 tcgtcagaac ctgctgcagg cagccgggaa cgtgggccag gccagtgggg agctgttgca
      961 gcaaattggg gaaagtgaca ctgaccccca cttccaggat gttctaatgc agctagcaaa
     1021 cgcagtggca agtgctgcag ccgccctggt cctcaaggcc aagagtgtgg cccagcgcac
     1081 agaggattcc gggcttcaga cccaagtgat tgctgcagct acgcaatgtg ctctgtccac
     1141 ttcccagctg gtggcctgta ccaaggtggt agcacctaca atcagctcac ctgtgtgcca
     1201 agaacagcta gtcgaggctg ggcgactggt ggccaaagct gtggagggct gtgtgtctgc
     1261 ctcgcaagca gctacagaag atggacagct gcttcgaggg gtaggagcag cagccacggc
     1321 tgtcacccag gccctcaatg agctgctgca gcacgtgaag gcccacgcca ccggagctgg
     1381 gcctgccggt cggtatgacc aagccactga taccatcctc accgtcactg aaaacatctt
     1441 cagctccatg ggtgatgctg gggagatggt gcgccaggcc cgcatcctgg cccaagccac
     1501 ctcagacctg gtcaatgcta tcaaggccga tgctgagggg gagagtgatc tggagaactc
     1561 tagaaagctc ctgagtgctg ccaagatcct cgctgatgcc accgccaaga tggtggaggc
     1621 ggccaaggga gcagccgccc accctgacag tgaggaacag cagcagcgac tgcgtgaagc
     1681 agctgagggg cttcgcatgg ccaccaatgc agctgcgcag aacgccatca agaagaagtt
     1741 ggtgcagcgc ctggagcatg cagccaagca agctgcagcc tctgcaacac agaccattgc
     1801 tgcagcccaa catgcagcct ctgcccccaa ggcctctgcg ggcccccagc ccctactggt
     1861 gcagagctgt aaggccgtgg cagagcagat tccgttgctg gtgcagggtg tccgaggaag
     1921 tcaagctcag cctgacagcc ctagtgctca gctcgccctc attgctgcca gccagagctt
     1981 cctgcagcca ggtgggaaga tggtggcagc ggcaaaagcc tcagtgccaa caattcagga
     2041 ccaggcttca gcgatgcagc tgagtcagtg cgcaaagaac ctaggcactg ccctggccga
     2101 gctgcgcact gctgctcaga aggctcagga ggcatgtgga cctttggaga tggattctgc
     2161 actgagtgtt gtacaaaatc tagagaaaga cctacaggaa ataaaagcag cagctcgaga
     2221 tggcaagctt aagcccttac ctggggaaac aatggagaag tgtacccaag atcttggcaa
     2281 cagcaccaaa gcggtgagct ctgccatcgc caagctgctg ggggagattg cccagggcaa
     2341 cgagaactat gcaggtattg cagctcggga tgtagctggt ggactaaggt cactagccca
     2401 ggctgcgcgg ggtgtggctg cgctgacatc agatcctgca gtgcaggcca ttgtgcttga
     2461 cacagccagc gacgttctgg acaaggccag cagcctcatt gaagaggcaa aaaaagcatc
     2521 tggacaccca ggggacccag aaagccagca aaggcttgct caggtagcta aagcagtgac
     2581 ccaagccctg aaccgctgtg tcagctgcct gcctggccaa agagacgtgg ataatgccct
     2641 acgagccgtt ggagatgcca gcaagcgact cctgagtgac ttgcttcctc caagcacggg
     2701 gacatttcaa gaagcacaga gccgattgaa tgaagctgca gctgggttaa atcaggccgc
     2761 cacagaactg gtgcaagcct cccgaggaac tcctcaggac ctggctcggg cctccggccg
     2821 atttggacag gacttcagca ccttcctgga agctggtgtg gagatggccg gacaggcccc
     2881 gagccaggag gaccgagccc aggtggtgtc caacctgaag ggcatatcca tgtcttcaag
     2941 caaacttctc cttgccgcta aggccttgtc cacagaccct gcttctccca acctcaagag
     3001 tcagctggct gcggctgccc gggcagtgac ggacagcatc aaccagctca tcaccatgtg
     3061 cacccagcag gcacctggcc agaaggagtg tgacaatgca cttcggcagc tagagacagt
     3121 ccgagaactc ctggagaatc cagtccagcc catcaacgac atgtcctact tcggttgctt
     3181 ggacagtgtc atggagaact ccaaggtcct aggtgaggcc atgactggca tctcccaaaa
     3241 tgccaagaat ggaaatctgc cggagtttgg agatgccatt gccacagcct ccaaggctct
     3301 ctgtggcttc acggaggcag ccgcacaggc agcatatcta gttggtgtct ctgaccccaa
     3361 cagccaagct ggacagcaag gactggtgga acccacacag tttgcccgtg caaaccaggc
     3421 aattcagatg gcctgtcaga gtctggggga gcctggctgt acccaggccc aggtgttatc
     3481 tgcagccact attgtagcca aacacacatc tgcattgtgt aacagctgtc gcctggcttc
     3541 cgctaggact gccaatccca ctgccaagcg ccagtttgta cagtcagcca aggaggtggc
     3601 caacagtaca gccaatcttg tcaagaccat caaggcacta gatggggact tcacagaaga
     3661 gaaccgtgcc cagtgccgag cagccacagc ccctctgctg gaagctgtgg ataacctgag
     3721 tgcctttgcc tccaaccctg agttctccag cgtccctgcc cagatcagcc ctgagggcag
     3781 agcagccatg gagcccattg taatctctgc taagacaatg ttggagagtg ctggagggct
     3841 catccagaca gctcgggcgt tagcagtcaa tccccgagac cccccacgtt ggtctgtgct
     3901 agctggccac tctcggactg tctcagattc catcaagaaa cttattacaa gcatgaggga
     3961 caaagcccca gggcagctgg agtgtgagac agccattgcg gctctgaaca gctgcttgcg
     4021 ggacctagat caggcttcgc ttgccgctgt cagccagcag cttgctcccc gtgaaggaat
     4081 ctctcaagag gctttgcaca cccagatgct cactgcagtg caagaaattt ctcatctcat
     4141 tgagccgctg gccagcgctg ctcgggctga agcctcccag ctgggacaca aggtgtccca
     4201 aatggcccag tactttgaac cactcaccct ggctgcagtg ggtgctgcgt ctaagaccct
     4261 gagccacccg cagcagatgg cacttctgga ccagactaaa acgttggcag agtctgcctt
     4321 gcagttgcta tatactgcca aggaggctgg tggtaacccc aagcaagcag cacacaccca
     4381 ggaagccctg gaggaggctg tgcagatgat gacagaggcc gtagaagacc tgacgacgac
     4441 cctcaacgaa gcagccagtg ctgcaggagt cgttggcggc atggtggact ctatcactca
     4501 ggccatcaac cagctagatg aaggacctat gggtgaccca gaaggctcat tcgtagatta
     4561 ccagacaacc atggtgagga cagccaaggc cattgctgtc actgttcagg agatggtaac
     4621 caagtcaaac accagccctg aagagctagg ccctcttgcc aaccagctga ccagtgacta
     4681 tggccgactg gcctcacaag ccaagcctgc agctgtggct gctgaaaatg aagagatagg
     4741 cgctcacatc aaacaccgag tacaggagct gggccatggc tgctctgctc tggtcaccaa
     4801 ggcaggtgcc ttgcagtgta gccccagtga tgtctacacc aagaaggagc tcatagagtg
     4861 tgcccgcaga gtgtcagaga aggtctccca tgttctggct gcactccagg ctgggaatcg
     4921 tggtacccag gcctgcatta cagcagccag tgctgtgtct ggtatcattg ctgacctcga
     4981 caccaccatc atgttcgcta ctgctggcac acttaaccgt gagggtgctg aaacttttgc
     5041 tgaccaccgg gagggtatct taaagacagc aaaggttctt gtggaggaca ccaaggtcct
     5101 agtgcagaat gcagctggga gccaggagaa gttggcacaa gccgcccagt cctccgtggc
     5161 caccattacc cgcctcgctg atgtggtcaa gctcggtgca gccagcctag gagccgaaga
     5221 ccctgaaact caggtggtgc tgatcaatgc agtaaaggac gtagccaagg ccctgggtga
     5281 cctcatcagc gctacgaagg ctgcagcggg caaagttggg gatgaccctg cagtgtggca
     5341 gctcaagaac tctgccaagg tgatggtgac caatgtgaca tcattgctca agacagtgaa
     5401 ggctgtggaa gatgaggcca ccaaaggcac acgggcccta gaggcaacca cagagcacat
     5461 acgtcaggaa ctggcggtct tctgttcccc agagccacct gccaagacct ctacccctga
     5521 agacttcatc cgaatgacca agggtattac tatggcaaca gccaaagccg ttgctgctgg
     5581 caattcctgt cgacaggaag atgtcattgc cacagccaat ctgagccgac gggctattgc
     5641 ggacatgctt cgggcttgca aggaagcagc tttccaccca gaagtggcgc ctgatgtacg
     5701 gctccgagcc ctgcactatg gccgggagtg tgccaatggt tacctggaac tgctggacca
     5761 cgtgctgctg acccttcaga agccaaaccc agacctgaag cagcagctaa ctggacactc
     5821 aaagcgggtt gctggctctg tgactgagct catccaggct gctgaagcca tgaaaggaac
     5881 agagtgggtg gacccagagg accctactgt cattgctgag aatgagctct tgggagccgc
     5941 agccgccatc gaggctgcag ccaagaagct ggagcagctg aagcccaggg ccaaacccaa
     6001 ggaggcggat gagtccttga actttgagga acaaatccta gaagctgcca agtccatcgc
     6061 tgcagccacc agtgcactgg taaaggctgc gtcagcagcc cagagggagc tggtggctca
     6121 aggaaaggtg ggcgccattc cagccaatgc actggatgat gggcagtggt cgcagggcct
     6181 catttccgct gcccgtatgg tggctgcggc caccaacaat ctgtgtgaag cagctaacgc
     6241 agctgtccag ggccatgcta gccaggagaa actcatttcc tcagccaagc aagtagctgc
     6301 ctccacagct cagctcctgg tagcttgcaa ggtcaaagca gatcaggact ctgaggcaat
     6361 gaaacggctc caggctgctg gcaatgcagt gaagagggcc tcagataacc tggtaaaggc
     6421 ggcccagaaa gctgcagcct tcgaagacca ggagaatgag acggtggtgg tgaaggagaa
     6481 aatggttggg ggcattgccc agattatcgc agcacaggaa gagatgcttc ggaaggaacg
     6541 agagctggaa gaggctcgga aaaagctcgc ccagatccgg cagcagcagt acaagttctt
     6601 gccttcagag cttcgagacg agcacatggt gagcaagggc gaggaggata acatggccat
     6661 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     6721 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     6781 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     6841 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     6901 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     6961 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     7021 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     7081 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     7141 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     7201 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     7261 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     7321 cgagctgtac aagtaaccgc gggcccggga tccaccggat ctagataact gatcataatc
     7381 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     7441 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     7501 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7561 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     7621 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     7681 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     7741 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     7801 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     7861 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     7921 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     7981 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     8041 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8101 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8161 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     8221 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8281 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8341 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8401 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8461 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8521 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     8581 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     8641 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     8701 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     8761 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     8821 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     8881 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     8941 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     9001 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     9061 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     9121 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     9181 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     9241 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     9301 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     9361 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     9421 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     9481 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     9541 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     9601 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     9661 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     9721 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     9781 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     9841 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9901 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9961 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10021 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10081 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10141 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10201 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10261 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10321 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10381 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10441 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10501 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10561 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10621 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10681 gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
    10741 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
    10801 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
    10861 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
    10921 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
    10981 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
    11041 cgctattacc atg
//
