LOCUS       sequence-267054-        5814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for dCas9 NLS, Part 3b(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM768
FEATURES             Location/Qualifiers
     source          1..5814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4200..4220
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(4255..4289)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4360..5019)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4934..4953
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5201..5789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5690..5709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
       61 ctgtggataa ccgtagtcgg tctcattctg acaagaagta ttctatcgga ctggccatcg
      121 ggactaatag cgtcgggtgg gccgtgatca ctgacgagta caaggtgccc tctaagaagt
      181 tcaaggtgct cgggaacacc gaccggcatt ccatcaagaa aaatctgatc ggagctctcc
      241 tctttgattc aggggaaacc gctgaagcaa cccgcctcaa gcggactgct agacggcggt
      301 acaccaggag gaagaaccgg atttgttacc ttcaagagat attctccaac gaaatggcaa
      361 aggtcgacga cagcttcttc cataggctgg aagaatcatt cctcgtggaa gaggataaga
      421 agcatgaacg gcatcccatc ttcggtaata tcgtcgacga ggtggcctat cacgagaaat
      481 acccaaccat ctaccatctt cgcaaaaagc tggtggactc aaccgacaag gcagacctcc
      541 ggcttatcta cctggccctg gcccacatga tcaagttcag aggccacttc ctgatcgagg
      601 gcgacctcaa tcctgacaat agcgatgtgg ataaactgtt catccagctg gtgcagactt
      661 acaaccagct ctttgaagag aaccccatca atgcaagcgg agtcgatgcc aaggccattc
      721 tgtcagcccg gctgtcaaag agccgcagac ttgagaatct tatcgctcag ctgccgggtg
      781 aaaagaaaaa tggactgttc gggaacctga ttgctctttc acttgggctg actcccaatt
      841 tcaagtctaa tttcgacctg gcagaggatg ccaagctgca actgtccaag gacacctatg
      901 atgacgatct cgacaacctc ctggcccaga tcggtgacca atacgccgac cttttccttg
      961 ctgctaagaa tctttctgac gccatcctgc tgtctgacat tctccgcgtg aacactgaaa
     1021 tcaccaaggc ccctctttca gcttcaatga ttaagcggta tgatgagcac caccaggacc
     1081 tgaccctgct taaggcactc gtccggcagc agcttccgga gaagtacaag gaaatcttct
     1141 ttgaccagtc aaagaatgga tacgccggct acatcgacgg aggtgcctcc caagaggaat
     1201 tttataagtt tatcaaacct atccttgaga agatggacgg caccgaagag ctcctcgtga
     1261 aactgaatcg ggaggatctg ctgcggaagc agcgcacttt cgacaatggg agcattcccc
     1321 accagatcca tcttggggag cttcacgcca tccttcggcg ccaagaggac ttctacccct
     1381 ttcttaagga caacagggag aagattgaga aaattctcac tttccgcatc ccctactacg
     1441 tgggacccct cgccagagga aatagccggt ttgcttggat gaccagaaag tcagaagaaa
     1501 ctatcactcc ctggaacttc gaagaggtgg tggacaaggg agccagcgct cagtcattca
     1561 tcgaacggat gactaacttc gataagaacc tccccaatga gaaggtcctg ccgaaacatt
     1621 ccctgctcta cgagtacttt accgtgtaca acgagctgac caaggtgaaa tatgtcaccg
     1681 aagggatgag gaagcccgca ttcctgtcag gcgaacaaaa gaaggcaatt gtggaccttc
     1741 tgttcaagac caatagaaag gtgaccgtga agcagctgaa ggaggactat ttcaagaaaa
     1801 ttgaatgctt cgactctgtg gagattagcg gggtcgaaga tcggttcaac gcaagcctgg
     1861 gtacctacca tgatctgctt aagatcatca aggacaagga ttttctggac aatgaggaga
     1921 aagaggacat ccttgaggac attgtcctga ctctcactct gttcgaggac cgggaaatga
     1981 tcgaggagag gcttaagacc tacgcccatc tgttcgacga taaagtgatg aagcaactta
     2041 aacggagaag atataccgga tggggacgcc ttagccgcaa actcatcaac ggaatccggg
     2101 acaaacagag cggaaagacc attcttgatt tccttaagag cgacggattc gctaatcgca
     2161 acttcatgca acttatccat gatgattccc tgacctttaa ggaggacatc cagaaggccc
     2221 aagtgtctgg acaaggtgac tcactgcacg agcatatcgc aaatctggct ggttcacccg
     2281 ctattaagaa gggtattctc cagaccgtga aagtcgtgga cgagctggtc aaggtgatgg
     2341 gtcgccataa accagagaac attgtcatcg agatggccag ggaaaaccag actacccaga
     2401 agggacagaa gaacagcagg gagcggatga aaagaattga ggaagggatt aaggagctcg
     2461 ggtcacagat ccttaaagag cacccggtgg aaaacaccca gcttcagaat gagaagctct
     2521 atctgtacta ccttcaaaat ggacgcgata tgtatgtgga ccaagagctt gatatcaaca
     2581 ggctctcaga ctacgacgtg gacgccatcg tccctcagag cttcctcaaa gacgactcaa
     2641 ttgacaataa ggtgctgact cgctcagaca agaaccgggg aaagtcagat aacgtgccct
     2701 cagaggaagt cgtgaaaaag atgaagaact attggcgcca gcttctgaac gcaaagctga
     2761 tcactcagcg gaagttcgac aatctcacta aggctgagag gggcggactg agcgaactgg
     2821 acaaagcagg attcattaaa cggcaacttg tggagactcg gcagattact aaacatgtag
     2881 cccaaatcct tgactcacgc atgaatacca agtacgacga aaacgacaaa cttatccgcg
     2941 aggtgaaggt gattaccctg aagtccaagc tggtcagcga tttcagaaag gactttcaat
     3001 tctacaaagt gcgggagatc aataactatc atcatgctca tgacgcatat ctgaatgccg
     3061 tggtgggaac cgccctgatc aagaagtacc caaagctgga aagcgagttc gtgtacggag
     3121 actacaaggt ctacgacgtg cgcaagatga ttgccaaatc tgagcaggag atcggaaagg
     3181 ccaccgcaaa gtacttcttc tacagcaaca tcatgaattt cttcaagacc gaaatcaccc
     3241 ttgcaaacgg tgagatccgg aagaggccgc tcatcgagac taatggggag actggcgaaa
     3301 tcgtgtggga caagggcaga gatttcgcta ccgtgcgcaa agtgctttct atgcctcaag
     3361 tgaacatcgt gaagaaaacc gaggtgcaaa ccggaggctt ttctaaggaa tcaatcctcc
     3421 ccaagcgcaa ctccgacaag ctcattgcaa ggaagaagga ttgggaccct aagaagtacg
     3481 gcggattcga ttcaccaact gtggcttatt ctgtcctggt cgtggctaag gtggaaaaag
     3541 gaaagtctaa gaagctcaag agcgtgaagg aactgctggg tatcaccatt atggagcgca
     3601 gctccttcga gaagaaccca attgactttc tcgaagccaa aggttacaag gaagtcaaga
     3661 aggaccttat catcaagctc ccaaagtata gcctgttcga actggagaat gggcggaagc
     3721 ggatgctcgc ctccgctggc gaacttcaga agggtaatga gctggctctc ccctccaagt
     3781 acgtgaattt cctctacctt gcaagccatt acgagaagct gaaggggagc cccgaggaca
     3841 acgagcaaaa gcaactgttt gtggagcagc ataagcatta tctggacgag atcattgagc
     3901 agatttccga gttttctaaa cgcgtcattc tcgctgatgc caacctcgat aaagtcctta
     3961 gcgcatacaa taagcacaga gacaaaccaa ttcgggagca ggctgagaat atcatccacc
     4021 tgttcaccct caccaatctt ggtgcccctg ccgcattcaa gtacttcgac accaccatcg
     4081 accggaaacg ctatacctcc accaaagaag tgctggacgc caccctcatc caccagagca
     4141 tcaccggact ttacgaaact cggattgacc tctcacagct cggaggggat gagggagctc
     4201 ccaagaaaaa gcgcaaggta ggtagttccg gatccggatc ctgagaccag accaataaaa
     4261 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     4321 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
     4381 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     4441 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     4501 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     4561 gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa
     4621 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     4681 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     4741 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     4801 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     4861 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     4921 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     4981 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     5041 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     5101 ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5161 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5221 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5281 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5341 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5401 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5461 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5521 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5581 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5641 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5701 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5761 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttac
//
