LOCUS       sequence-267094-        8478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Env gene from HIV patient plasma in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pHP022Env
FEATURES             Location/Qualifiers
     source          1..8478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        147..526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        527..730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     681..701
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     775..794
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        775..793
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2672..2905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3428..3454
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3888..3929
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3939..3956
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3979..3996)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3985..4209
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4255..4683
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4342..4361)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4552..4573
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4692..4712)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4697..5026
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4877..5012
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4939..4958
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5093..5887
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5147..5166)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5757..5776
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6063..6184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6100..6119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6154..6173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6233..6249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6233..6249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6246..6268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6257..6273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6281..6287,6288..6305,6306..6311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6326..6347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6464..6481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6635..7223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6715..6734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7394..8185,8186..8254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8017..8036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8255..8359)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8434..8453)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
       61 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
      121 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      181 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      361 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      421 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      541 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      601 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      661 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      721 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      781 gactcactat agggagaccc aagctggcta gttaagcttg gtaccgagct cggatccagt
      841 acccttcacc gatcaagctt taggcatctc ctatggcagg aagaagcgga gacagcgacg
      901 aagagctcct caagacagcc aggctcatca agtttctcta tcaaagcagt aagtagtaca
      961 tgtaatgcaa cctttagtaa tattatcaat agtagcatta gtagtagtaa caataatagc
     1021 aataattgta tggtccatag tactcataga atataggaaa atattaaaag aaagaaaaat
     1081 agaaaggtta attgatagaa taagagaaag agcagaagac agtggcaatg acagtgacgg
     1141 ggaccaggaa gaattatcaa cacttgtgga gatggggcac catgctcctt gggatattaa
     1201 tgatctgtag tgctgcagga cagttgtggg tcacagtcta ttatggggta cctgtgtgga
     1261 aagacgcaac caccacttta ttttgtgcat cagatgctaa ggcatatgag acagaagtac
     1321 ataatgtttg ggccacacat gcctgtgtac ccacagaccc caacccacaa gaaatagtaa
     1381 tggaaaatgt gacagaaaat tttaacatgt ggaaaaacta tatggtagaa caaatgcatg
     1441 aggatataat cagtttatgg gatcaaagtc taaagccatg tgtaaaatta accccacttt
     1501 gtgttaggct aaattgcagt gatcctaaga atagtactaa tatcagtaat ggtactactg
     1561 ccaataatag tactgttggc ccctccgttt tagaagaagt aagaaactgc tctttcaata
     1621 tctccacaaa cctaaatgaa aagagacaaa aagaatatgc actttttgct aaacttgatg
     1681 tagagtcaat aggcaatggt actaccatgt acaggttggt gcaatgtaac acctcaacca
     1741 ttacacaggc ctgtccaaag gtatcctttg atccaattcc catacattat tgtgccccgg
     1801 ctggttttgc gattataaag tgtaaccagg aaaatttcaa tggaacaggg ccatgtaaaa
     1861 atgtcagcac agtacaatgt acacatggaa ttaagccagt agtatcaact caactgctgt
     1921 taaatggcag tctagcagaa gatgagatag taattagatc tgaaaatttc acaaacaatg
     1981 ttaaaaccat aatagtacag ctgaatgaat ctgtagaaat taattgtaca agacccaaca
     2041 acaatacaag aagaagtata catttaggat tcgggagggc attgtataca acaggagaaa
     2101 taacaggaga tataagaaaa gcccattgca acattagtag acaaaaatgg aataacactt
     2161 taaaacagat agttagcaaa ttaaaagaac aatttaataa aacaatagtc cttcagccgc
     2221 cctccccagg aggagaccca gaaattgtaa tgcacagttt tacttgtgga ggagaatttt
     2281 tctactgtaa tacatcacaa ctgtttaata gtacttggaa tagtactact gaccatacta
     2341 accttacagg agatgcaatt atcacgctcc catgcagaat aaaacaaatt ataaacatgt
     2401 ggcaaggagt aggaagagca atgtatgccc ctcccatcag aggacaaatt aactgttcat
     2461 caaatattac agggctgcta ttaacaagag atggtggtgt taacaatacc accgataata
     2521 ataccgagat cttcagacca ggaggaggag atatgaggga caattggaga agtgaattat
     2581 ataaatataa agtggtaaaa attgaaccat taggaatagc acccaccaag gcaaagagaa
     2641 gagtggtgca tagagaaaga agagcggcac taggagctat gttccttggg ttcttgggag
     2701 cagcaggaag cactatgggc gcagcgtcag tgacgctgac ggtacaggcc agactattat
     2761 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcata
     2821 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagagtcctg gctgtggaaa
     2881 gatacctaag ggatcaacag ctcctaggaa tttggggttg ctctggaaaa ctcatctgca
     2941 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggatgag atttggaata
     3001 acatgacctg gatggagtgg gaaagagaaa ttgacaacta cacaggctta atatacaact
     3061 tacttgaaca atcgcagaac cagcaagata agaatgaaca ggaattatta gagttaaata
     3121 aatgggcaaa tttgtggaat tggtttgaca taacaaattg gctatggtat ataaaaatat
     3181 tcataatgat agtaggaggc ttaataggtt taagaatagt ttttgctgtg ctttctatag
     3241 tgaatagagt taggcaggga tactcaccaa tctcgtttca gacccccttc ccagccccga
     3301 ggggacccga caggcccgga ggaacagaag aagaaggtgg agagagagac agagacagat
     3361 ccgctcgatt agtgaacgga ttcttgccac ttatctggga cgacttgagg agcctgtgcc
     3421 tcttcagcta ccaccgcttg agagacttac tcttgattgt aacgaggatt gtggaacttc
     3481 tgggacgcag ggggtggcaa cttctcaagt attggtggaa cctcctccag tattggagtc
     3541 aggaactaag gaatagcgct attagcttgc tcaacgcaac agccatagca gtaggtgagg
     3601 gaacagatag ggttttagaa gcattacaga gagcttgtag agctatccgc aacatacctg
     3661 taagagtaag acaaggcttg gaaaggcttt tgctataaag atgggtggca agtggtcaaa
     3721 aagtgtacgt ggatggccta ctgtaaggga aagaatgaga caagctgagc cagcagcaga
     3781 tggggtggga gcagtatctc gagacggatc cagctaaggg tcaagacaat tctgcagata
     3841 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
     3901 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
     3961 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4021 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4081 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4141 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     4201 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     4261 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4321 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4381 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4441 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4501 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4561 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4621 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4681 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4741 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4801 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4861 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4921 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4981 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     5041 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     5101 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5161 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5221 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5281 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5341 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5401 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5461 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5521 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5581 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5641 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5701 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5761 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5821 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5881 cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat
     5941 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6001 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6061 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6121 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6181 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6301 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6421 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8401 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8461 aagccagtat ctgctccc
//
