LOCUS       sequence-267121-        2104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for Linus, Part 4a(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM582
FEATURES             Location/Qualifiers
     source          1..2104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(559..593)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(664..1323)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1238..1257
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1505..2093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1994..2013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
       61 gttatcccct gattctgtgg ataaccgtag tcggtctcaa tccttggcta ctacacttga
      121 acgtattgag aagaactttg tcattactga cccaagattg ccagataatc ccattatatt
      181 cgcgtccgat agtttcttgc agttgacaga atatagccgt gaagaaattt tgggaagaaa
      241 ctgcaggttt ctacaaggtc ctgaaactga tcgcgcgaca gtgagaaaaa ttagagatgc
      301 catagataac caaacagagg tcactgttca gctgattaat tatacaaaga gtggtaaaaa
      361 gttctggaac ctctttcact tgcagcctat gcgagatcag aagggagatg tccagtactt
      421 tattggggtt cagttggatg gaactgagca tgtccgagat gctgccgaga gagagggagt
      481 catgctgatt aagaaaactg cagaagagat tgccaaaaag aagagaaagg tctaactcga
      541 gtggctgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      601 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
      661 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
      721 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
      781 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
      841 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat
      901 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
      961 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     1021 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     1081 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     1141 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     1201 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     1261 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     1321 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     1381 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa
     1441 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1501 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1561 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1621 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1681 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1741 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1801 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1861 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1921 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1981 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2041 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2101 gcaa
//
