LOCUS       sequence-267122-        5814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for dCas9 NLS, Part 3(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM767
FEATURES             Location/Qualifiers
     source          1..5814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4206..4226
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(4261..4295)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4366..5025)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4940..4959
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5207..5795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5696..5715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
       61 ctgattctgt ggataaccgt agtcggtctc atatggacaa gaagtattct atcggactgg
      121 ccatcgggac taatagcgtc gggtgggccg tgatcactga cgagtacaag gtgccctcta
      181 agaagttcaa ggtgctcggg aacaccgacc ggcattccat caagaaaaat ctgatcggag
      241 ctctcctctt tgattcaggg gaaaccgctg aagcaacccg cctcaagcgg actgctagac
      301 ggcggtacac caggaggaag aaccggattt gttaccttca agagatattc tccaacgaaa
      361 tggcaaaggt cgacgacagc ttcttccata ggctggaaga atcattcctc gtggaagagg
      421 ataagaagca tgaacggcat cccatcttcg gtaatatcgt cgacgaggtg gcctatcacg
      481 agaaataccc aaccatctac catcttcgca aaaagctggt ggactcaacc gacaaggcag
      541 acctccggct tatctacctg gccctggccc acatgatcaa gttcagaggc cacttcctga
      601 tcgagggcga cctcaatcct gacaatagcg atgtggataa actgttcatc cagctggtgc
      661 agacttacaa ccagctcttt gaagagaacc ccatcaatgc aagcggagtc gatgccaagg
      721 ccattctgtc agcccggctg tcaaagagcc gcagacttga gaatcttatc gctcagctgc
      781 cgggtgaaaa gaaaaatgga ctgttcggga acctgattgc tctttcactt gggctgactc
      841 ccaatttcaa gtctaatttc gacctggcag aggatgccaa gctgcaactg tccaaggaca
      901 cctatgatga cgatctcgac aacctcctgg cccagatcgg tgaccaatac gccgaccttt
      961 tccttgctgc taagaatctt tctgacgcca tcctgctgtc tgacattctc cgcgtgaaca
     1021 ctgaaatcac caaggcccct ctttcagctt caatgattaa gcggtatgat gagcaccacc
     1081 aggacctgac cctgcttaag gcactcgtcc ggcagcagct tccggagaag tacaaggaaa
     1141 tcttctttga ccagtcaaag aatggatacg ccggctacat cgacggaggt gcctcccaag
     1201 aggaatttta taagtttatc aaacctatcc ttgagaagat ggacggcacc gaagagctcc
     1261 tcgtgaaact gaatcgggag gatctgctgc ggaagcagcg cactttcgac aatgggagca
     1321 ttccccacca gatccatctt ggggagcttc acgccatcct tcggcgccaa gaggacttct
     1381 acccctttct taaggacaac agggagaaga ttgagaaaat tctcactttc cgcatcccct
     1441 actacgtggg acccctcgcc agaggaaata gccggtttgc ttggatgacc agaaagtcag
     1501 aagaaactat cactccctgg aacttcgaag aggtggtgga caagggagcc agcgctcagt
     1561 cattcatcga acggatgact aacttcgata agaacctccc caatgagaag gtcctgccga
     1621 aacattccct gctctacgag tactttaccg tgtacaacga gctgaccaag gtgaaatatg
     1681 tcaccgaagg gatgaggaag cccgcattcc tgtcaggcga acaaaagaag gcaattgtgg
     1741 accttctgtt caagaccaat agaaaggtga ccgtgaagca gctgaaggag gactatttca
     1801 agaaaattga atgcttcgac tctgtggaga ttagcggggt cgaagatcgg ttcaacgcaa
     1861 gcctgggtac ctaccatgat ctgcttaaga tcatcaagga caaggatttt ctggacaatg
     1921 aggagaaaga ggacatcctt gaggacattg tcctgactct cactctgttc gaggaccggg
     1981 aaatgatcga ggagaggctt aagacctacg cccatctgtt cgacgataaa gtgatgaagc
     2041 aacttaaacg gagaagatat accggatggg gacgccttag ccgcaaactc atcaacggaa
     2101 tccgggacaa acagagcgga aagaccattc ttgatttcct taagagcgac ggattcgcta
     2161 atcgcaactt catgcaactt atccatgatg attccctgac ctttaaggag gacatccaga
     2221 aggcccaagt gtctggacaa ggtgactcac tgcacgagca tatcgcaaat ctggctggtt
     2281 cacccgctat taagaagggt attctccaga ccgtgaaagt cgtggacgag ctggtcaagg
     2341 tgatgggtcg ccataaacca gagaacattg tcatcgagat ggccagggaa aaccagacta
     2401 cccagaaggg acagaagaac agcagggagc ggatgaaaag aattgaggaa gggattaagg
     2461 agctcgggtc acagatcctt aaagagcacc cggtggaaaa cacccagctt cagaatgaga
     2521 agctctatct gtactacctt caaaatggac gcgatatgta tgtggaccaa gagcttgata
     2581 tcaacaggct ctcagactac gacgtggacg ccatcgtccc tcagagcttc ctcaaagacg
     2641 actcaattga caataaggtg ctgactcgct cagacaagaa ccggggaaag tcagataacg
     2701 tgccctcaga ggaagtcgtg aaaaagatga agaactattg gcgccagctt ctgaacgcaa
     2761 agctgatcac tcagcggaag ttcgacaatc tcactaaggc tgagaggggc ggactgagcg
     2821 aactggacaa agcaggattc attaaacggc aacttgtgga gactcggcag attactaaac
     2881 atgtagccca aatccttgac tcacgcatga ataccaagta cgacgaaaac gacaaactta
     2941 tccgcgaggt gaaggtgatt accctgaagt ccaagctggt cagcgatttc agaaaggact
     3001 ttcaattcta caaagtgcgg gagatcaata actatcatca tgctcatgac gcatatctga
     3061 atgccgtggt gggaaccgcc ctgatcaaga agtacccaaa gctggaaagc gagttcgtgt
     3121 acggagacta caaggtctac gacgtgcgca agatgattgc caaatctgag caggagatcg
     3181 gaaaggccac cgcaaagtac ttcttctaca gcaacatcat gaatttcttc aagaccgaaa
     3241 tcacccttgc aaacggtgag atccggaaga ggccgctcat cgagactaat ggggagactg
     3301 gcgaaatcgt gtgggacaag ggcagagatt tcgctaccgt gcgcaaagtg ctttctatgc
     3361 ctcaagtgaa catcgtgaag aaaaccgagg tgcaaaccgg aggcttttct aaggaatcaa
     3421 tcctccccaa gcgcaactcc gacaagctca ttgcaaggaa gaaggattgg gaccctaaga
     3481 agtacggcgg attcgattca ccaactgtgg cttattctgt cctggtcgtg gctaaggtgg
     3541 aaaaaggaaa gtctaagaag ctcaagagcg tgaaggaact gctgggtatc accattatgg
     3601 agcgcagctc cttcgagaag aacccaattg actttctcga agccaaaggt tacaaggaag
     3661 tcaagaagga ccttatcatc aagctcccaa agtatagcct gttcgaactg gagaatgggc
     3721 ggaagcggat gctcgcctcc gctggcgaac ttcagaaggg taatgagctg gctctcccct
     3781 ccaagtacgt gaatttcctc taccttgcaa gccattacga gaagctgaag gggagccccg
     3841 aggacaacga gcaaaagcaa ctgtttgtgg agcagcataa gcattatctg gacgagatca
     3901 ttgagcagat ttccgagttt tctaaacgcg tcattctcgc tgatgccaac ctcgataaag
     3961 tccttagcgc atacaataag cacagagaca aaccaattcg ggagcaggct gagaatatca
     4021 tccacctgtt caccctcacc aatcttggtg cccctgccgc attcaagtac ttcgacacca
     4081 ccatcgaccg gaaacgctat acctccacca aagaagtgct ggacgccacc ctcatccacc
     4141 agagcatcac cggactttac gaaactcgga ttgacctctc acagctcgga ggggatgagg
     4201 gagctcccaa gaaaaagcgc aaggtaggta gttccggatc cggatcctga gaccagacca
     4261 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     4321 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     4381 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     4441 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     4501 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     4561 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
     4621 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     4681 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     4741 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     4801 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     4861 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     4921 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     4981 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     5041 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     5101 aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt
     5161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5341 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5581 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcct
//
