LOCUS       sequence-267127-        8752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression fusion protein ALKBH5-dCas9 in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Liu XM, Zhou J, Mao Y, Ji Q, Qian SB
  TITLE     Programmable RNA N(6)-methyladenosine editing by CRISPR-Cas9 
            conjugates.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):865-871. doi: 
            10.1038/s41589-019-0327-1. Epub 2019 Aug 5.
  PUBMED    31383972
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0327-1"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "865-871"
COMMENT     Sequence Label: ALKBH5-dCas9
FEATURES             Location/Qualifiers
     source          1..8752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      652..661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      656..665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1888..5286,5287..5289,5290..5886,5887,5888..5890,
                     5891..5892,5893..5895,5896..5988)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1888..1911,1912..1914,1915..4401,4402..4404,
                     4405..5988)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5989..6009
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6016..6033
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6056..6073)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6062..6286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6357..6373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6357..6373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6370..6392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6381..6397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6405..6411,6412..6429,6430..6435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6450..6471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6588..6605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6759..7347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6839..6858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7518..8309,8310..8378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8141..8160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8379..8483)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8562..8581)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 gcggccgcca gcggctacac ggacctgcgt gagaagctca agtccatgac gtcccgggac
      721 aactataagg cgggcagccg ggaggccgcc gccgctgccg cagccgccgt agccgccgca
      781 gccgcagccg ccgctgccgc cgaaccttac cctgtgtccg gggccaagcg caagtatcag
      841 gaggactcgg accccgagcg cagcgactat gaggagcagc agctgcagaa ggaggaggag
      901 gcgcgcaagg tgaagagcgg catccgccag atgcgcctct tcagccagga cgagtgcgcc
      961 aagatcgagg cccgcattga cgaggtggtg tcccgcgctg agaagggcct gtacaacgag
     1021 cacacggtgg accgggcccc actgcgcaac aagtacttct tcggcgaagg ctacacttac
     1081 ggcgcccagc tgcagaagcg cgggcccggc caggagcgcc tctacccgcc gggcgacgtg
     1141 gacgagatcc ccgagtgggt gcaccagctg gtgatccaaa agctggtgga gcaccgcgtc
     1201 atccccgagg gcttcgtcaa cagcgccgtc atcaacgact accagcccgg cggctgcatc
     1261 gtgtctcacg tggaccccat ccacatcttc gagcgcccca tcgtgtccgt gtccttcttt
     1321 agcgactctg cgctgtgctt cggctgcaag ttccagttca agcctattcg ggtgtcggaa
     1381 ccagtgcttt ccctgccggt gcgcagggga agcgtgactg tgctcagtgg atatgctgct
     1441 gatgaaatca ctcactgcat acggcctcag gacatcaagg agcgccgagc agtcatcatc
     1501 ctcaggaaga caagattaga tgcaccccgg ttggaaacaa agtccctgag cagctccgtg
     1561 ttaccaccca gctatgcttc agatcgcctg tcaggaaaca acagggaccc tgctctgaaa
     1621 cccaagcggt cccaccgcaa ggcagaccct gatgctgccc acaggccacg gatcctggag
     1681 atggacaagg aagagaaccg gcgctcggtg ctgctgccca cacaccggcg gaggggtagc
     1741 ttcagctctg agaactactg gcgcaagtca tacgagtcct cagaggactg ctctgaggca
     1801 gcaggcagcc ctgcccgaaa ggtgaagatg cggcggcaca gcggcagcga gactcccggg
     1861 acctcagagt ccgccacacc cgaaagtgat aaaaagtatt ctattggttt agccatcggc
     1921 actaattccg ttggatgggc tgtcataacc gatgaataca aagtaccttc aaagaaattt
     1981 aaggtgttgg ggaacacaga ccgtcattcg attaaaaaga atcttatcgg tgccctccta
     2041 ttcgatagtg gcgaaacggc agaggcgact cgcctgaaac gaaccgctcg gagaaggtat
     2101 acacgtcgca agaaccgaat atgttactta caagaaattt ttagcaatga gatggccaaa
     2161 gttgacgatt ctttctttca ccgtttggaa gagtccttcc ttgtcgaaga ggacaagaaa
     2221 catgaacggc accccatctt tggaaacata gtagatgagg tggcatatca tgaaaagtac
     2281 ccaacgattt atcacctcag aaaaaagcta gttgactcaa ctgataaagc ggacctgagg
     2341 ttaatctact tggctcttgc ccatatgata aagttccgtg ggcactttct cattgagggt
     2401 gatctaaatc cggacaactc ggatgtcgac aaactgttca tccagttagt acaaacctat
     2461 aatcagttgt ttgaagagaa ccctataaat gcaagtggcg tggatgcgaa ggctattctt
     2521 agcgcccgcc tctctaaatc ccgacggcta gaaaacctga tcgcacaatt acccggagag
     2581 aagaaaaatg ggttgttcgg taaccttata gcgctctcac taggcctgac accaaatttt
     2641 aagtcgaact tcgacttagc tgaagatgcc aaattgcagc ttagtaagga cacgtacgat
     2701 gacgatctcg acaatctact ggcacaaatt ggagatcagt atgcggactt atttttggct
     2761 gccaaaaacc ttagcgatgc aatcctccta tctgacatac tgagagttaa tactgagatt
     2821 accaaggcgc cgttatccgc ttcaatgatc aaaaggtacg atgaacatca ccaagacttg
     2881 acacttctca aggccctagt ccgtcagcaa ctgcctgaga aatataagga aatattcttt
     2941 gatcagtcga aaaacgggta cgcaggttat attgacggcg gagcgagtca agaggaattc
     3001 tacaagttta tcaaacccat attagagaag atggatggga cggaagagtt gcttgtaaaa
     3061 ctcaatcgcg aagatctact gcgaaagcag cggactttcg acaacggtag cattccacat
     3121 caaatccact taggcgaatt gcatgctata cttagaaggc aggaggattt ttatccgttc
     3181 ctcaaagaca atcgtgaaaa gattgagaaa atcctaacct ttcgcatacc ttactatgtg
     3241 ggacccctgg cccgagggaa ctctcggttc gcatggatga caagaaagtc cgaagaaacg
     3301 attactccat ggaattttga ggaagttgtc gataaaggtg cgtcagctca atcgttcatc
     3361 gagaggatga ccaactttga caagaattta ccgaacgaaa aagtattgcc taagcacagt
     3421 ttactttacg agtatttcac agtgtacaat gaactcacga aagttaagta tgtcactgag
     3481 ggcatgcgta aacccgcctt tctaagcgga gaacagaaga aagcaatagt agatctgtta
     3541 ttcaagacca accgcaaagt gacagttaag caattgaaag aggactactt taagaaaatt
     3601 gaatgcttcg attctgtcga gatctccggg gtagaagatc gatttaatgc gtcacttggt
     3661 acgtatcatg acctcctaaa gataattaaa gataaggact tcctggataa cgaagagaat
     3721 gaagatatct tagaagatat agtgttgact cttaccctct ttgaagatcg ggaaatgatt
     3781 gaggaaagac taaaaacata cgctcacctg ttcgacgata aggttatgaa acagttaaag
     3841 aggcgtcgct atacgggctg gggacgattg tcgcggaaac ttatcaacgg gataagagac
     3901 aagcaaagtg gtaaaactat tctcgatttt ctaaagagcg acggcttcgc caataggaac
     3961 tttatgcagc tgatccatga tgactcttta accttcaaag aggatataca aaaggcacag
     4021 gtttccggac aaggggactc attgcacgaa catattgcga atcttgctgg ttcgccagcc
     4081 atcaaaaagg gcatactcca gacagtcaaa gtagtggatg agctagttaa ggtcatggga
     4141 cgtcacaaac cggaaaacat tgtaatcgag atggcacgcg aaaatcaaac gactcagaag
     4201 gggcaaaaaa acagtcgaga gcggatgaag agaatagaag agggtattaa agaactgggc
     4261 agccagatct taaaggagca tcctgtggaa aatacccaat tgcagaacga gaaactttac
     4321 ctctattacc tacaaaatgg aagggacatg tatgttgatc aggaactgga cataaaccgt
     4381 ttatctgatt acgacgtcga tgccattgta ccccaatcct ttttgaagga cgattcaatc
     4441 gacaataaag tgcttacacg ctcggataag aaccgaggga aaagtgacaa tgttccaagc
     4501 gaggaagtcg taaagaaaat gaagaactat tggcggcagc tcctaaatgc gaaactgata
     4561 acgcaaagaa agttcgataa cttaactaaa gctgagaggg gtggcttgtc tgaacttgac
     4621 aaggccggat ttattaaacg tcagctcgtg gaaacccgcc aaatcacaaa gcatgttgca
     4681 cagatactag attcccgaat gaatacgaaa tacgacgaga acgataagct gattcgggaa
     4741 gtcaaagtaa tcactttaaa gtcaaaattg gtgtcggact tcagaaagga ttttcaattc
     4801 tataaagtta gggagataaa taactaccac catgcgcacg acgcttatct taatgccgtc
     4861 gtagggaccg cactcattaa gaaatacccg aagctagaaa gtgagtttgt gtatggtgat
     4921 tacaaagttt atgacgtccg taagatgatc gcgaaaagcg aacaggagat aggcaaggct
     4981 acagccaaat acttctttta ttctaacatt atgaatttct ttaagacgga aatcactctg
     5041 gcaaacggag agatacgcaa acgaccttta attgaaacca atggggagac aggtgaaatc
     5101 gtatgggata agggccggga cttcgcgacg gtgagaaaag ttttgtccat gccccaagtc
     5161 aacatagtaa agaaaactga ggtgcagacc ggagggtttt caaaggaatc gattcttcca
     5221 aaaaggaata gtgataagct catcgctcgt aaaaaggact gggacccgaa aaagtacggt
     5281 ggcttcgata gccctacagt tgcctattct gtcctagtag tggcaaaagt tgagaaggga
     5341 aaatccaaga aactgaagtc agtcaaagaa ttattgggga taacgattat ggagcgctcg
     5401 tcttttgaaa agaaccccat cgacttcctt gaggcgaaag gttacaagga agtaaaaaag
     5461 gatctcataa ttaaactacc aaagtatagt ctgtttgagt tagaaaatgg ccgaaaacgg
     5521 atgttggcta gcgccggaga gcttcaaaag gggaacgaac tcgcactacc gtctaaatac
     5581 gtgaatttcc tgtatttagc gtcccattac gagaagttga aaggttcacc tgaagataac
     5641 gaacagaagc aactttttgt tgagcagcac aaacattatc tcgacgaaat catagagcaa
     5701 atttcggaat tcagtaagag agtcatccta gctgatgcca atctggacaa agtattaagc
     5761 gcatacaaca agcacaggga taaacccata cgtgagcagg cggaaaatat tatccatttg
     5821 tttactctta ccaacctcgg cgctccagcc gcattcaagt attttgacac aacgatagat
     5881 cgcaaacgat acacttctac caaggaggtg ctagacgcga cactgattca ccaatccatc
     5941 acgggattat atgaaactcg gatagatttg tcacagcttg ggggtgaccc caagaagaag
     6001 aggaaagtca ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
     6061 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6121 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6181 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6241 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6301 agaaccagct ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6361 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6421 agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6481 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6541 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7501 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7561 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7621 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7681 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7741 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7801 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7861 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7921 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7981 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8041 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8101 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8161 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8221 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8281 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8341 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8401 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8461 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     8521 tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc
     8581 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     8641 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     8701 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
