LOCUS       sequence-267129-        2263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for Linus-Mxi, Part 4a(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM725
FEATURES             Location/Qualifiers
     source          1..2263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             541..663
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     terminator      complement(690..724)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(795..1454)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1369..1388
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1636..2224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2125..2144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
       61 agtcggtctc aatccttggc tactacactt gaacgtattg agaagaactt tgtcattact
      121 gacccaagat tgccagataa tcccattata ttcgcgtccg atagtttctt gcagttgaca
      181 gaatatagcc gtgaagaaat tttgggaaga aactgcaggt ttctacaagg tcctgaaact
      241 gatcgcgcga cagtgagaaa aattagagat gccatagata accaaacaga ggtcactgtt
      301 cagctgatta attatacaaa gagtggtaaa aagttctgga acctctttca cttgcagcct
      361 atgcgagatc agaagggaga tgtccagtac tttattgggg ttcagttgga tggaactgag
      421 catgtccgag atgctgccga gagagaggga gtcatgctga ttaagaaaac tgcagaagag
      481 attgccaaaa agaagagaaa ggtcggcggc agcggcggcg gcagcggcag cggcggcagc
      541 atggaacgtg tgagaatgat taatgtgcaa aggctgttag aagccgcaga gtttttagaa
      601 agaagagaaa gagaatgcga acacgggtat gccagttctt tccctagcat gccctctccc
      661 agataactcg agtggctgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc
      721 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
      781 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      841 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
      901 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
      961 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa
     1021 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1081 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1141 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1201 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1261 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1321 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1381 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1441 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1501 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat
     1561 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1621 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1681 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1741 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1801 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1861 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1921 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1981 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2041 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2101 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2161 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2221 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttt
//
