LOCUS       sequence-267131-        2104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for ERG25 sgRNA, Part 234(promoter,coding region, 
            terminator).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM756
FEATURES             Location/Qualifiers
     source          1..2104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        303..378
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(475..509)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(580..1239)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1154..1173
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1421..2009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1910..1929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgtagtcgg tctccaacga tgtgcttcag tattacattt tttgccttca acgccttgat
       61 tgttctattt ttgctaataa taaatctatt tcatcggact aaaagtccat tagttgtaag
      121 cggatttagc tcagttggga gagcgccaga ctgaagaaaa acttcggtca agtcatctgg
      181 aggtcctgtg ttcgatccac agaattcgca gatggccggc atggtcccag cctcctcgct
      241 ggcgccggct gggcaacacc ttcgggtggc gaatgggact ttatatagaa gtatgcatac
      301 acgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      361 gtggcaccga gtcggtgctt tttttatttt ttgtcactat tgttatgtaa aatgccacct
      421 ctgacagtat ggaacgcaaa cttctgtcta gtggatagct gtgagaccag accaataaaa
      481 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
      541 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
      601 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
      661 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
      721 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
      781 gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa
      841 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
      901 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
      961 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     1021 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     1081 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     1141 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     1201 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     1261 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     1321 ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1381 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1441 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1501 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1561 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1621 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1681 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1741 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1801 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1861 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1921 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1981 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2041 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2101 ataa
//
