LOCUS       sequence-267132-        5817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for dCas9 NLS Stop, Part 3(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM765
FEATURES             Location/Qualifiers
     source          1..5817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4194..4214
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(4252..4286)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4357..5016)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4931..4950
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5198..5786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5687..5706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
       61 ataaccgtag tcggtctcat atggacaaga agtattctat cggactggcc atcgggacta
      121 atagcgtcgg gtgggccgtg atcactgacg agtacaaggt gccctctaag aagttcaagg
      181 tgctcgggaa caccgaccgg cattccatca agaaaaatct gatcggagct ctcctctttg
      241 attcagggga aaccgctgaa gcaacccgcc tcaagcggac tgctagacgg cggtacacca
      301 ggaggaagaa ccggatttgt taccttcaag agatattctc caacgaaatg gcaaaggtcg
      361 acgacagctt cttccatagg ctggaagaat cattcctcgt ggaagaggat aagaagcatg
      421 aacggcatcc catcttcggt aatatcgtcg acgaggtggc ctatcacgag aaatacccaa
      481 ccatctacca tcttcgcaaa aagctggtgg actcaaccga caaggcagac ctccggctta
      541 tctacctggc cctggcccac atgatcaagt tcagaggcca cttcctgatc gagggcgacc
      601 tcaatcctga caatagcgat gtggataaac tgttcatcca gctggtgcag acttacaacc
      661 agctctttga agagaacccc atcaatgcaa gcggagtcga tgccaaggcc attctgtcag
      721 cccggctgtc aaagagccgc agacttgaga atcttatcgc tcagctgccg ggtgaaaaga
      781 aaaatggact gttcgggaac ctgattgctc tttcacttgg gctgactccc aatttcaagt
      841 ctaatttcga cctggcagag gatgccaagc tgcaactgtc caaggacacc tatgatgacg
      901 atctcgacaa cctcctggcc cagatcggtg accaatacgc cgaccttttc cttgctgcta
      961 agaatctttc tgacgccatc ctgctgtctg acattctccg cgtgaacact gaaatcacca
     1021 aggcccctct ttcagcttca atgattaagc ggtatgatga gcaccaccag gacctgaccc
     1081 tgcttaaggc actcgtccgg cagcagcttc cggagaagta caaggaaatc ttctttgacc
     1141 agtcaaagaa tggatacgcc ggctacatcg acggaggtgc ctcccaagag gaattttata
     1201 agtttatcaa acctatcctt gagaagatgg acggcaccga agagctcctc gtgaaactga
     1261 atcgggagga tctgctgcgg aagcagcgca ctttcgacaa tgggagcatt ccccaccaga
     1321 tccatcttgg ggagcttcac gccatccttc ggcgccaaga ggacttctac ccctttctta
     1381 aggacaacag ggagaagatt gagaaaattc tcactttccg catcccctac tacgtgggac
     1441 ccctcgccag aggaaatagc cggtttgctt ggatgaccag aaagtcagaa gaaactatca
     1501 ctccctggaa cttcgaagag gtggtggaca agggagccag cgctcagtca ttcatcgaac
     1561 ggatgactaa cttcgataag aacctcccca atgagaaggt cctgccgaaa cattccctgc
     1621 tctacgagta ctttaccgtg tacaacgagc tgaccaaggt gaaatatgtc accgaaggga
     1681 tgaggaagcc cgcattcctg tcaggcgaac aaaagaaggc aattgtggac cttctgttca
     1741 agaccaatag aaaggtgacc gtgaagcagc tgaaggagga ctatttcaag aaaattgaat
     1801 gcttcgactc tgtggagatt agcggggtcg aagatcggtt caacgcaagc ctgggtacct
     1861 accatgatct gcttaagatc atcaaggaca aggattttct ggacaatgag gagaaagagg
     1921 acatccttga ggacattgtc ctgactctca ctctgttcga ggaccgggaa atgatcgagg
     1981 agaggcttaa gacctacgcc catctgttcg acgataaagt gatgaagcaa cttaaacgga
     2041 gaagatatac cggatgggga cgccttagcc gcaaactcat caacggaatc cgggacaaac
     2101 agagcggaaa gaccattctt gatttcctta agagcgacgg attcgctaat cgcaacttca
     2161 tgcaacttat ccatgatgat tccctgacct ttaaggagga catccagaag gcccaagtgt
     2221 ctggacaagg tgactcactg cacgagcata tcgcaaatct ggctggttca cccgctatta
     2281 agaagggtat tctccagacc gtgaaagtcg tggacgagct ggtcaaggtg atgggtcgcc
     2341 ataaaccaga gaacattgtc atcgagatgg ccagggaaaa ccagactacc cagaagggac
     2401 agaagaacag cagggagcgg atgaaaagaa ttgaggaagg gattaaggag ctcgggtcac
     2461 agatccttaa agagcacccg gtggaaaaca cccagcttca gaatgagaag ctctatctgt
     2521 actaccttca aaatggacgc gatatgtatg tggaccaaga gcttgatatc aacaggctct
     2581 cagactacga cgtggacgcc atcgtccctc agagcttcct caaagacgac tcaattgaca
     2641 ataaggtgct gactcgctca gacaagaacc ggggaaagtc agataacgtg ccctcagagg
     2701 aagtcgtgaa aaagatgaag aactattggc gccagcttct gaacgcaaag ctgatcactc
     2761 agcggaagtt cgacaatctc actaaggctg agaggggcgg actgagcgaa ctggacaaag
     2821 caggattcat taaacggcaa cttgtggaga ctcggcagat tactaaacat gtagcccaaa
     2881 tccttgactc acgcatgaat accaagtacg acgaaaacga caaacttatc cgcgaggtga
     2941 aggtgattac cctgaagtcc aagctggtca gcgatttcag aaaggacttt caattctaca
     3001 aagtgcggga gatcaataac tatcatcatg ctcatgacgc atatctgaat gccgtggtgg
     3061 gaaccgccct gatcaagaag tacccaaagc tggaaagcga gttcgtgtac ggagactaca
     3121 aggtctacga cgtgcgcaag atgattgcca aatctgagca ggagatcgga aaggccaccg
     3181 caaagtactt cttctacagc aacatcatga atttcttcaa gaccgaaatc acccttgcaa
     3241 acggtgagat ccggaagagg ccgctcatcg agactaatgg ggagactggc gaaatcgtgt
     3301 gggacaaggg cagagatttc gctaccgtgc gcaaagtgct ttctatgcct caagtgaaca
     3361 tcgtgaagaa aaccgaggtg caaaccggag gcttttctaa ggaatcaatc ctccccaagc
     3421 gcaactccga caagctcatt gcaaggaaga aggattggga ccctaagaag tacggcggat
     3481 tcgattcacc aactgtggct tattctgtcc tggtcgtggc taaggtggaa aaaggaaagt
     3541 ctaagaagct caagagcgtg aaggaactgc tgggtatcac cattatggag cgcagctcct
     3601 tcgagaagaa cccaattgac tttctcgaag ccaaaggtta caaggaagtc aagaaggacc
     3661 ttatcatcaa gctcccaaag tatagcctgt tcgaactgga gaatgggcgg aagcggatgc
     3721 tcgcctccgc tggcgaactt cagaagggta atgagctggc tctcccctcc aagtacgtga
     3781 atttcctcta ccttgcaagc cattacgaga agctgaaggg gagccccgag gacaacgagc
     3841 aaaagcaact gtttgtggag cagcataagc attatctgga cgagatcatt gagcagattt
     3901 ccgagttttc taaacgcgtc attctcgctg atgccaacct cgataaagtc cttagcgcat
     3961 acaataagca cagagacaaa ccaattcggg agcaggctga gaatatcatc cacctgttca
     4021 ccctcaccaa tcttggtgcc cctgccgcat tcaagtactt cgacaccacc atcgaccgga
     4081 aacgctatac ctccaccaaa gaagtgctgg acgccaccct catccaccag agcatcaccg
     4141 gactttacga aactcggatt gacctctcac agctcggagg ggatgaggga gctcccaaga
     4201 aaaagcgcaa ggtaggtagt tccggatcct agggatcctg agaccagacc aataaaaaac
     4261 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     4321 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     4381 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     4441 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     4501 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     4561 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
     4621 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     4681 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     4741 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     4801 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     4861 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     4921 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     4981 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     5041 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     5101 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
     5161 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5221 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5281 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5341 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5401 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5461 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5521 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5581 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5641 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5701 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5761 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcc
//
