LOCUS       sequence-267135-       10609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mEGFP expression regulated by the CaMV35S promoter, co-expressed 
            hygromycin phosphotransferase selectable marker (hpt).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10609)
  AUTHORS   Behrendorff JBYH, Borras-Gas G, Pribil M
  TITLE     Antimicrobial solid media for screening non-sterile Arabidopsis 
            thaliana seeds.
  JOURNAL   Physiol Plant. 2020 Feb 25. doi: 10.1111/ppl.13079.
  PUBMED    32096870
REFERENCE   2  (bases 1 to 10609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Physiol 
            Plant. 2020 Feb 25. doi: 10.1111/ppl.13079."
COMMENT     Sequence Label: pH_35S/mEGFP
FEATURES             Location/Qualifiers
     source          1..10609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(453..455,456..458,459..1172)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(498..519)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(759..778)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1106..1127
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1175..1199)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1519..1543
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2874..3503
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3932..5005
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5071..5265
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5501..5523)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5609..5749
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5664..5683
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5764..5781)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5935..6523)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6015..6034)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6769..7560)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8081..8105
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8198..8450)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8648..9673)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9709..9888)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttatggtgag caagggcgag gagctgttca
      481 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      541 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      601 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      661 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      721 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      781 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      841 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      901 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      961 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1021 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
     1081 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1141 tcactctcgg catggacgag ctgtacaagt aatcgttcag ctttcttgta caaagtggtg
     1201 atatcccgcg gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
     1261 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     1321 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     1381 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgcaggc
     1441 atgcgacgtc gggcccaagc ttagcttgag cttggatcag attgtcgttt cccgccttca
     1501 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     1561 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     1621 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttaaagt actttaaagt
     1681 actttaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     1741 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     1801 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     1861 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     1921 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     1981 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     2041 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     2101 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     2161 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     2221 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     2281 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     2341 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     2401 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     2461 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     2521 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     2581 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     2641 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     2701 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     2761 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     2821 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     2881 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     2941 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     3001 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     3061 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     3121 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     3181 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     3241 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     3301 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     3361 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     3421 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     3481 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     3541 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     3601 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     3661 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     3721 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     3781 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     3841 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
     3901 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     3961 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     4021 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     4081 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     4141 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     4201 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     4261 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     4321 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     4381 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     4441 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     4501 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     4561 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     4621 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     4681 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     4741 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     4801 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     4861 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     4921 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     4981 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     5041 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     5101 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     5161 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     5221 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     5281 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     5341 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     5401 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     5461 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     5521 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     5581 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     5641 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     5701 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     5761 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5821 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5881 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5941 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6001 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6061 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6121 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6181 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6241 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6301 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6361 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6421 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6481 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6541 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca
     6601 tgatatatct cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga
     6661 cttgacctga tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta
     6721 agccgcgccg cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac
     6781 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga
     6841 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
     6901 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     6961 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     7021 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     7081 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     7141 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     7201 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
     7261 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
     7321 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
     7381 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
     7441 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
     7501 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat
     7561 gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
     7621 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
     7681 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     7741 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt
     7801 acgaaccgaa caggcttatg tccactgggt tcgtgcccga attgatcaca ggcagcaacg
     7861 ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg
     7921 gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta
     7981 caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt
     8041 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
     8101 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa
     8161 ttaacgccga attgaattat cagcttgcat gccggtcgat ctagtaacat agatgacacc
     8221 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     8281 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     8341 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     8401 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc tgcttgactc
     8461 tagggaatta attcctgaat cactgcgacc ggccctcccg cgacccagcc gagcgagctt
     8521 agcgaactgt ggacgagaac tgtgccacca agcgtaaggc cgttctctcg catttgcctt
     8581 gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa tcagctcttg ttcggtcggc
     8641 atctactcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga
     8701 gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc
     8761 cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat
     8821 catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat
     8881 acgcccggag ccttggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct
     8941 gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg
     9001 aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca
     9061 ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg
     9121 cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca
     9181 cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag
     9241 tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg
     9301 ccgcagcgat agcatccata gcctccgcga ccggctgaag aacagcgggc agttcggttt
     9361 caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct
     9421 cgctgaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc
     9481 gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc
     9541 cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca
     9601 ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt
     9661 caggcttttt catagggggg attcgagttg agagtgaata tgagactcta attggatacc
     9721 gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata
     9781 gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat
     9841 taaactccag aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg tcagttccaa
     9901 acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc
     9961 cgctcatgat cgataattcg agctctccca tatggtcgac tagagccaag ctgatctcct
    10021 ttgccccgga gatcaccatg gacgactttc tctatctcta cgatctagga agaaagttcg
    10081 acggagaagg tgacgatacc atgttcacca ccgataatga gaagattagc ctcttcaatt
    10141 tcagaaagaa tgctgaccca cagatggtta gagaggccta cgcggcaggt ctcatcaaga
    10201 cgatctaccc gagtaataat ctccaggaga tcaaatacct tcccaagaag gttaaagatg
    10261 cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga
    10321 tcagaagtac tattccagta tggacgattc aaggcttgct tcataaacca aggcaagtaa
    10381 tagagattgg agtctctaag aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa
    10441 ttcagatcga ggatctaaca gaactcgccg tgaagactgg cgaacagttc atacagagtc
    10501 ttttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctcg
    10561 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctat
//
