LOCUS       sequence-267148-       12951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector coding Cas9 and mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12951)
  AUTHORS   Huang Y, Mouttet B, Warnatz HJ, Risch T, Rietmann F, Frommelt F, Ngo
            QA, Dobay MP, Marovca B, Jenni S, Tsai YC, Matzk S, Amstislavskiy V,
            Schrappe M, Stanulla M, Gstaiger M, Bornhauser B, Yaspo ML, Bourquin
            JP
  TITLE     The Leukemogenic TCF3-HLF Complex Rewires Enhancers Driving Cellular
            Identity and Self-Renewal Conferring EP300 Vulnerability.
  JOURNAL   Cancer Cell. 2019 Dec 9;36(6):630-644.e9. doi: 
            10.1016/j.ccell.2019.10.004. Epub 2019 Nov 14.
  PUBMED    31735627
REFERENCE   2  (bases 1 to 12951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2019.10.004"; journalName: "Cancer Cell"; date: 
            "2019-12-9- 9"; volume: "36"; issue: "6"; pages: "630-644.e9"
COMMENT     Sequence Label: pL40C_PGKintron_Cas9_Green
FEATURES             Location/Qualifiers
     source          1..12951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        437..628
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(460..480)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        647..873
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             874..1054
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1101..1226
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1719..1952
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2137..2181
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2479..2596
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2641..3151
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3070..3088
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3160..3732
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3753..3762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3762..3785
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3792..3812
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3837..7937
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3837..7937
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7938..7985
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8004..8060
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      8316..8325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8787..9375
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8840..8860)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9258..9269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9447..9680
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9758..9879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9795..9814)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9849..9868
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9919..10054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10074..10093)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10075..10093)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10103..10120)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10103..10119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10112..10134)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10261..10716
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10348..10367)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10558..10579
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10742..10846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10847..10915,10916..11707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11065..11084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11878..12466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12367..12386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12620..12637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12754..12775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12790..12795,12796..12813,12814..12820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12828..12844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12833..12855
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12852..12868
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12852..12868
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12887..12907
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12889..12907
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gcgcgccatg cagggcctga aataacctct gaaagaggaa
      421 cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
      481 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      541 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      601 atctcaatta gtcagcaacc atagtccctt aagaggcgcg ccttaatgta gtcttatgca
      661 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      721 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      781 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      841 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      901 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      961 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     1021 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     1081 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     1141 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     1201 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     1261 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1321 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1381 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1441 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1501 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1561 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1621 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1681 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1741 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1801 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1861 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1921 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1981 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     2041 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     2101 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     2161 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     2221 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2281 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2341 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2401 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     2461 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2521 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2581 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa
     2641 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     2701 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     2761 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
     2821 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
     2881 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
     2941 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
     3001 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
     3061 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
     3121 cgttgaccga atcaccgacc tctctcccca gggggatctg tgagtttggg gacccttgat
     3181 tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg
     3241 gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt
     3301 ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct
     3361 gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt
     3421 tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata
     3481 ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga
     3541 atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac
     3601 atcctggtca tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg
     3661 aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc
     3721 tttttcctac agctcctggg caacgtggat ccgccaccat ggattacaaa gacgatgacg
     3781 ataagatggc cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccgaca
     3841 agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg
     3901 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
     3961 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
     4021 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
     4081 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
     4141 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
     4201 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
     4261 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
     4321 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
     4381 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     4441 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     4501 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg
     4561 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     4621 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     4681 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     4741 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     4801 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     4861 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     4921 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     4981 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     5041 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     5101 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     5161 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     5221 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     5281 acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     5341 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg
     5401 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     5461 agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     5521 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     5581 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     5641 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     5701 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     5761 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     5821 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     5881 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     5941 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     6001 acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg
     6061 tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa
     6121 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     6181 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     6241 acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt
     6301 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc
     6361 ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga
     6421 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     6481 ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg
     6541 ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg
     6601 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt
     6661 acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg
     6721 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc
     6781 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta
     6841 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg
     6901 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca
     6961 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga
     7021 tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg
     7081 tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag
     7141 gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa
     7201 agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg
     7261 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc
     7321 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg
     7381 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc
     7441 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg
     7501 gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg
     7561 agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca
     7621 agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg
     7681 ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca
     7741 gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg
     7801 ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc
     7861 tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt
     7921 ctcagctggg aggcgacaag cgtcctgctg ctactaagaa agctggtcaa gctaagaaaa
     7981 agaaagctag cggaggaagc ggcgccacca atttcagcct gctgaaacag gccggcgacg
     8041 tggaagagaa ccctggccct atggtgtcca agggcgaaga ggacaacatg gccagcctgc
     8101 ctgccaccca cgagctgcac atcttcggca gcatcaacgg cgtggacttc gacatggtgg
     8161 gacagggcac cggcaacccc aacgacggct acgaggaact gaacctgaag tccacaaagg
     8221 gcgacctgca gttcagcccc tggattctgg tgccccacat cggctacggc ttccaccagt
     8281 acctgcccta ccccgacggc atgagccctt tccaagccgc catggtggat ggcagcggct
     8341 accaggtgca ccggaccatg cagtttgagg acggcgccag cctgaccgtg aactacaggt
     8401 acacatacga gggcagccac atcaagggcg aggcccaagt gaagggcaca ggctttccag
     8461 ccgacggccc cgtgatgacc aatagcctga cagccgccga ctggtgcaga agcaagaaaa
     8521 cctaccccaa tgacaagacc atcatcagca ccttcaagtg gtcctacacc accggcaatg
     8581 gcaagcggta cagaagcacc gcccggacca cctacacctt cgccaaaccc atggccgcca
     8641 actacctgaa gaaccagcct atgtacgtgt tccgcaagac cgagctgaag cactccaaga
     8701 cagaactgaa cttcaaagag tggcagaaag ccttcaccga cgtgatgggc atggacgagc
     8761 tgtacaagtg aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     8821 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     8881 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     8941 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     9001 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     9061 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     9121 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     9181 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     9241 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     9301 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     9361 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     9421 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     9481 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9541 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9601 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9661 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     9721 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     9781 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     9901 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9961 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    10021 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
    10081 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
    10141 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
    10201 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
    10261 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    10321 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    10381 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    10441 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    10501 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    10561 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    10621 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    10681 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
    10741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    10801 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
    10861 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
    10921 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
    10981 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
    11041 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
    11101 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
    11161 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
    11221 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
    11281 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
    11341 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
    11401 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
    11461 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
    11521 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
    11581 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
    11641 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    11701 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    11761 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    11821 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
    11881 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
    11941 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
    12001 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
    12061 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
    12121 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
    12181 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
    12241 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
    12301 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
    12361 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
    12421 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
    12481 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
    12541 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
    12601 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
    12661 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    12721 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    12781 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    12841 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
    12901 ctaaagggaa caaaagctgg agctgcaagc ggtaccgtcg acctgcaggt t
//
