LOCUS       sequence-267149-       12690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK13 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12690)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-CDK13_IDG-K
FEATURES             Location/Qualifiers
     source          1..12690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2826..2835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2835..2858
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2868..2892
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5449..5460
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7459..7483)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          7557..7786
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7769..7788
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(7859..8433,8434..8436,8437..8445)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8026..8043)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8253..8272
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8465..9064
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8465..8484)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8961..8981
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9079..9667
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9132..9152)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9550..9561)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9742..9975
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10094..10112)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10180..10284
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10285..10353,10354..11145)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10503..10522)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11316..11904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11805..11824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12058..12075
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12316..12337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12352..12357,12358..12375,12376..12382)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12390..12406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12395..12417
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12414..12430
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12414..12430
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12480..12499
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccat accggtcagc
     2821 tcgaggccac catggactac aaggacgacg acgacaaggg cggatcaaca agtttgtaca
     2881 aaaaagcaga agggccgtca aggcccacca tgccgagcag ctcggacacg gcgctggggg
     2941 gaggcggggg cctgagctgg gcggagaaga agttggagga acgccgcaag cggaggcgat
     3001 tcctgtcccc tcagcagccg ccgctgctgt tgccgctcct gcagccgcag ctcctgcaac
     3061 cgccgccgcc cccgccgcct ctgctcttcc tggctgctcc cggcacggcc gccgccgcag
     3121 ccgccgccgc cgcggcctcc tcctcttgct tcagcccggg cccccctctg gaggtcaagc
     3181 ggctggcgag aggcaagagg cgcgcaggag ggcggcagaa gcggcgtcgc gggccccgcg
     3241 ccgggcagga ggcggagaag cgtcgggtct tctcgctgcc ccagccgcag caggacggcg
     3301 gtggcggtgc tagtagcggc gggggtgtga ccccgctggt ggaatacgag gatgtgagct
     3361 cccagtccga gcaggggctg ctgctggggg gggccagcgc ggcaacggcg gcgacggctg
     3421 ccgggggaac ggggggcagc ggcgggagtc cggcctcctc ctccggcacc cagcggcgcg
     3481 gggaggggtc ggagcgcagg ccccgccggg accgccgcag cagcagtggc cgcagcaagg
     3541 agcgccaccg cgagcaccgg cggcgggatg ggcagcgcgg tggcagcgag gcctccaagt
     3601 cccgcagccg ccacagccac agcggcgagg aacgggccga ggtcgccaag agcggcagca
     3661 gcagcagcag cggcggccgc cggaaaagcg cttcggccac atccagcagc agtagcagcc
     3721 gcaaggaccg ggactcgaag gcccaccgca gccggactaa gtcgtccaag gagccgcctt
     3781 cggcctacaa ggaaccgccc aaggcctacc gggaggacaa gaccgagcct aaggcctaca
     3841 ggcggcggcg gtccctcagc ccactgggag gccgggacga cagcccggtg tcccacaggg
     3901 cctctcagag cctgaggagc cgcaagtccc ccagcccggc aggaggtggc agcagcccct
     3961 attctcggcg gctgccgcgc tccccgagcc cctacagtcg ccgccgctcc cccagctaca
     4021 gccgccacag ctcctacgag cggggcggcg acgtgtcccc tagtccctac agcagcagca
     4081 gctggcgccg ctctcgcagt ccctacagcc ctgtgctcag acggtctgga aaatcccgaa
     4141 gcagaagccc gtattcatct aggcattcaa gatctcgtag caggcacaga ttgtctagat
     4201 ccagaagtcg tcattctagt atttctccta gcacactaac tctgaagagt agcctggcag
     4261 ctgaattgaa caagaataaa aaagcacgag cagcagaggc agcaagagcc gcagaagcag
     4321 cgaaagctgc agaagcaact aaggctgctg aggctgctgc caaggctgca aaagcttcaa
     4381 acacttctac acctaccaag gggaacacgg aaactagtgc cagtgcatca caaacaaacc
     4441 atgtgaagga tgtgaagaaa attaaaattg aacatgcacc ttctccctca agtggtggaa
     4501 ctttaaaaaa tgacaaagca aaaacaaagc cacctcttca ggtaacgaag gtggaaaata
     4561 atttgattgt agataaagcc accaagaaag cagtcatagt tggaaaggag agtaaatctg
     4621 ctgctacaaa ggaggaatca gtatctctta aagagaaaac caaaccactt acaccaagca
     4681 taggagccaa ggagaaggag caacatgtag ctttagtcac ctctacatta ccaccgttac
     4741 ctttgcctcc catgctgcct gaagataaag aagctgatag cttacgagga aatatttcag
     4801 taaaagcagt taaaaaagaa gtagaaaaga aactccgatg tcttcttgct gatttaccgc
     4861 tgccccctga gctaccagga ggagatgatc tttcaaagag tccagaggaa aagaaaacag
     4921 caacacagtt acatagtaaa aggaggccta aaatatgtgg gcctcgctat ggtgaaacca
     4981 aagaaaaaga tattgactgg ggaaaacgct gcgtggataa atttgatatc atcggaatta
     5041 ttggagaagg tacttacgga caagtttaca aagccaggga taaagacact ggagaaatgg
     5101 tagccttaaa aaaagtacgt ctggataatg aaaaggaagg ctttccaatt acagcaattc
     5161 gagaaattaa aattctccgg cagcttaccc atcagagtat tatcaatatg aaggaaatag
     5221 tgactgataa agaagatgct ttggatttca agaaggacaa aggtgcattt tatctggtgt
     5281 ttgaatatat ggaccatgat ctgatgggac tactggaatc aggcttggtt cattttaatg
     5341 aaaatcacat aaagtcattt atgagacagc tcatggaggg tctggattat tgtcataaga
     5401 agaacttttt gcatagagat attaaatgtt ccaatatcct tctaaataat agagggcaga
     5461 taaaacttgc agactttgga cttgctcgat tgtatagctc agaagaaagt cggccgtata
     5521 ctaacaaggt aattacttta tggtaccgtc cacctgaact gctactggga gaagaacgat
     5581 acacaccagc cattgatgta tggagctgtg gctgtatcct tggcgaactc ttcactaaaa
     5641 aacctatatt tcaagcaaat caggaacttg cacaactaga attaataagc cgaatatgtg
     5701 ggagtccatg tcctgcagtg tggcctgatg taatcaaact accatatttc aacaccatga
     5761 aaccaaagaa gcaatatcgt cgaaagttaa gagaagaatt tgtttttatt cctgcagctg
     5821 cgctagactt atttgattac atgcttgcct tggatcctag taagcgctgc actgctgaac
     5881 aggctcttca gtgcgagttc ctccgagatg tggaaccctc aaaaatgcct ccaccagatc
     5941 tccctttatg gcaagattgt catgagttat ggagtaaaaa gcgaagaaga cagaagcaga
     6001 tgggcatgac tgatgatgtt tccacaatta aagcccccag gaaggacttg tctctgggct
     6061 tggatgacag cagaaccaac acaccccagg gtgtgctgcc atcttcacag ctgaaatctc
     6121 agggcagctc aaatgtggca cctgtaaaaa caggccctgg acagcactta aaccacagtg
     6181 aattggcaat tctactaaac ctactacaat ctaaaacaag tgttaatatg gctgattttg
     6241 tccaagtgtt gaacattaag gtaaactctg agactcaaca gcagctaaat aaaataaacc
     6301 ttcctgctgg aattttggca acaggtgaaa aacagacaga tccatcaaca ccacaacagg
     6361 agtcttcgaa accgttggga ggaattcagc cttcttctca gaccatccag cctaaagtgg
     6421 agactgatgc tgcccaggcg gctgtgcaga gtgcatttgc agttctgttg actcagttaa
     6481 taaaggctca gcagtcaaag cagaaagatg tgctactaga agagagggaa aatggatcgg
     6541 gacatgaagc gtcattacaa ctcaggccac ctccagaacc tagcactccg gtgtcgggac
     6601 aagatgacct catccagcat caagatatga ggatcttgga gctaacgcca gaaccagacc
     6661 ggcctcgaat tctgcctcct gaccaacgac ctcccgagcc tcctgaacca ccaccagtca
     6721 ctgaggaaga tctagattat cggacagaaa accagcatgt acccaccacc agttcttcat
     6781 taactgaccc tcatgccgga gtgaaggcag ccctgttaca gctgcttgct cagcatcagc
     6841 cccaggatga ccccaaaaga gaaggtggga ttgattatca agcaggagac acttacgtgt
     6901 ccacttcaga ctacaaggac aactttggat cctcttcttt ctcttctgct ccttatgtta
     6961 gcaatgatgg tctaggaagc agttctgctc caccactaga acgacgtagt ttcattggaa
     7021 attcagatat tcagtctttg gataactaca gtactgcttc atctcattct ggtggtccac
     7081 ctcagccttc tgccttttct gagtcatttc ccagttcagt agctggatat ggagacattt
     7141 acctcaatgc tggtcccatg ttgtttagtg gagacaagga ccatagattt gaatatagcc
     7201 atggtcctat tgcagtcctg gcaaacagca gtgacccttc cacggggcca gagagtactc
     7261 atcctttgcc agcaaagatg cacaactata actatggtgg taacttacag gaaaatccga
     7321 gtggccccag cctcatgcat ggacagacct ggacttctcc tgcccaagga cctggatatt
     7381 cacaaggata caggggacat attagcacat caactggcag aggcagaggc agagggttac
     7441 catactaggg cctcatgggc ccagctttct tgtacaaagt ggttgattaa gatccactag
     7501 taacggccgc cagtgtgctg gaattaattc gctgtctgcg agggccagct gttggggtga
     7561 gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga
     7621 ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg cgtccatctg
     7681 gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc
     7741 atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt ccactcccag
     7801 gtccaactgc aggtcgagca tgcatctagg gcggccaatt ccgcccctct cccccccccc
     7861 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     7921 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     7981 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     8041 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     8101 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     8161 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     8221 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     8281 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     8341 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     8401 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc
     8461 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     8521 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     8581 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8641 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     8701 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8761 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8821 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     8881 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     8941 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9001 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9061 ctgatatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     9121 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     9181 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     9241 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     9301 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     9361 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     9421 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     9481 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     9541 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     9601 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     9661 tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat cttagccact
     9721 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     9781 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     9841 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     9901 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     9961 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
    10021 agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct
    10081 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
    10141 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    10201 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    10261 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    10321 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    10381 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    10441 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    10501 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
    10561 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    10621 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    10681 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    10741 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    10801 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    10861 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    10921 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    10981 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    11041 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    11101 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    11161 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    11221 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    11281 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    11341 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    11401 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    11461 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    11521 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    11581 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    11641 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    11701 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    11761 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    11821 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    11881 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    11941 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    12001 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    12061 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    12121 tggccgattc attaatgcag caagctcatg gctgactaat ttttttttat ttatgcagag
    12181 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    12241 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
    12301 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    12361 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    12421 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
    12481 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    12541 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    12601 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    12661 ttgttaaata tgtactacaa acttagtagt
//
