LOCUS       sequence-267151-        9617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with N-terminal 
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9617)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-PKMYT1_IDG-K
FEATURES             Location/Qualifiers
     source          1..9617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2826..2835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2835..2858
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2868..2892
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4390..4414)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          4488..4717
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4700..4719
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4897..5359
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4953..4970)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5180..5199
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5392..5991
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5392..5411)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5888..5908
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6006..6594
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6059..6079)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6477..6488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6669..6902
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7021..7039)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7107..7211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7212..7280,7281..8072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7430..7449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8243..8831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8732..8751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8985..9002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9243..9264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9279..9284,9285..9302,9303..9309)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9317..9333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9322..9344
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9341..9357
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9341..9357
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9407..9426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccat accggtcagc
     2821 tcgaggccac catggactac aaggacgacg acgacaaggg cggatcaaca agtttgtaca
     2881 aaaaagttgg caccatgcta gaacggcctc ctgcactggc catgcccatg cccacggagg
     2941 gcaccccgcc acctctgagt ggcaccccca tcccagtccc agcctacttc cgccacgcag
     3001 aacctggatt ctccctcaag aggcccaggg ggctcagccg gagcctccca cctccgcccc
     3061 ctgccaaggg cagcattccc atcagccgcc tcttccctcc tcggacccca ggctggcacc
     3121 agctgcagcc ccggcgggtg tcattccggg gcgaggcctc agagactctg cagagccctg
     3181 ggtatgaccc aagccggcca gagtccttct tccagcagag cttccagagg ctcagccgcc
     3241 tgggccatgg ctcctacgga gaggtcttca aggtgcgctc caaggaggac ggccggctct
     3301 atgcggtaaa gcgttccatg tcaccattcc ggggccccaa ggaccgggcc cgcaagttgg
     3361 ccgaggtggg cagccacgag aaggtggggc agcacccatg ctgcgtgcgg ctggagcagg
     3421 cctgggagga gggcggcatc ctgtacctgc agacggagct gtgcgggccc agcctgcagc
     3481 aacactgtga ggcctggggt gccagcctgc ctgaggccca ggtctggggc tacctgcggg
     3541 acacgctgct tgccctggcc catctgcaca gccagggcct ggtgcacctt gatgtcaagc
     3601 ctgccaacat cttcctgggg ccccggggcc gctgcaagct gggtgacttc ggactgctgg
     3661 tggagctggg tacagcagga gctggtgagg tccaggaggg agacccccgc tacatggccc
     3721 ccgagctgct gcagggctcc tatgggacag cagcggatgt gttcagtctg ggcctcacca
     3781 tcctggaagt ggcatgcaac atggagctgc cccacggtgg ggagggctgg cagcagctgc
     3841 gccagggcta cctgccccct gagttcactg ccggtctgtc ttccgagctg cgttctgtcc
     3901 ttgtcatgat gctggagcca gaccccaagc tgcgggccac ggccgaggcc ctgctggcac
     3961 tgcctgtgtt gaggcagccg cgggcctggg gtgtgctgtg gtgcatggca gcggaggccc
     4021 tgagccgagg gtgggccctg tggcaggccc tgcttgccct gctctgctgg ctctggcatg
     4081 ggctggctca ccctgccagc tggctacagc ccctgggccc gccagccacc ccgcctggct
     4141 caccaccctg cagtttgctc ctggacagca gcctctccag caactgggat gacgacagcc
     4201 tagggccttc actctcccct gaggctgtcc tggcccggac tgtggggagc acctccaccc
     4261 cccggagcag gtgcacaccc agggatgccc tggacctaag tgacatcaac tcagagcctc
     4321 ctcggggctc cttcccctcc tttgagcctc ggaacctcct cagcctgttt gaggacaccc
     4381 tagacccaac cccaactttc ttgtacaaag tggttgatta agatccacta gtaacggccg
     4441 ccagtgtgct ggaattaatt cgctgtctgc gagggccagc tgttggggtg agtactccct
     4501 ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt
     4561 gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa
     4621 gacaatcttt ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg
     4681 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg
     4741 caggtcgagc atgcatctag ggcggccaat tccgcccctc tccccccccc ccctctcccc
     4801 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     4861 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     4921 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     4981 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     5041 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     5101 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     5161 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     5221 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     5281 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     5341 ttgaaaaaca cgatgataag cttgccacaa cccacaagga gacgaccttc catgaccgag
     5401 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc
     5461 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc
     5521 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5581 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5641 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5701 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     5761 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     5821 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     5881 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     5941 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg atatcgatag
     6001 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6061 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6121 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6181 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6241 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6301 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6361 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6421 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6481 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6541 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga
     6601 tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6661 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     6721 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6781 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6841 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6901 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6961 agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg
     7021 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     7081 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7141 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7201 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7261 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7321 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7381 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7441 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7501 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7561 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7621 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7681 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7741 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7801 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7861 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7921 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7981 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8041 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8101 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8161 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8221 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8281 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8341 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8401 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8461 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8521 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8581 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8641 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8701 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8761 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8821 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8881 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8941 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9001 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9061 aatgcagcaa gctcatggct gactaatttt tttttattta tgcagaggcc gaggccgcct
     9121 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     9181 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     9241 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     9301 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
     9361 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9421 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     9481 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     9541 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     9601 actacaaact tagtagt
//
