LOCUS       sequence-267157-       11201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TPC1 with C-terminus mCherry tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Ruas M, Davis LC, Chen CC, Morgan AJ, Chuang KT, Walseth TF, Grimm 
            C, Garnham C, Powell T, Platt N, Platt FM, Biel M, Wahl-Schott C, 
            Parrington J, Galione A
  TITLE     Expression of Ca(2)(+)-permeable two-pore channels rescues NAADP 
            signalling in TPC-deficient cells.
  JOURNAL   EMBO J. 2015 Jul 2;34(13):1743-58. doi: 10.15252/embj.201490009. 
            Epub 2015 Apr 14.
  PUBMED    25872774
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201490009"; journalName: "EMBO J"; date: "2015-07-2- 
            2"; volume: "34"; issue: "13"; pages: "1743-58"
COMMENT     Sequence Label: TPC1-mCherry
FEATURES             Location/Qualifiers
     source          1..11201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    501..734
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             919..963
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1226..1341
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        1399..1702
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1703..1906
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1857..1877
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1903..1927
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4498..5205
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNLAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4640..4658)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4908..4927
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        5234..5733
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5269..5292)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5666..5685
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5754..6353
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5754..5773)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6250..6270
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6367..6955
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6420..6440)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6838..6849)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7162..7795
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7924..7940)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7924..7940)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7937..7959)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7948..7964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7972..7978,7979..7996,7997..8002))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8017..8038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8155..8172)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8326..8914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8406..8425)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9085..9876,9877..9945))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9708..9727
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9946..10050)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10098..10232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10135..10154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10189..10208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10400..11033
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    join(11080..11201,1..4)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
       61 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
      121 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
      181 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
      241 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
      301 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
      361 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
      421 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
      481 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
      541 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
      601 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
      661 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
      721 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
      781 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
      841 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
      901 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
      961 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     1021 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     1081 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     1141 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     1201 gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg gggattgggg ggtacagtgc
     1261 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     1321 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttatcg
     1381 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1441 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     1501 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     1561 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     1621 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     1681 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     1741 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1801 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     1861 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     1921 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     1981 ctagaggatc gctagcgcta ccggactcag atctcgagct caagcttcga attcaccatg
     2041 gctgtaagtt tagatgacga tgtgccgctc atcctgacct tggacgaggc tgagagtgct
     2101 ccgctgcctc cttcgaacag cctgggccaa gagcagctgc ccagcaaaaa tgggggcagc
     2161 cacagcatcc acaactccca ggtccccagt ctggtctccg gagcggacag ccccccctcc
     2221 agtcccaccg gacacaactg ggagatgaat tatcaagagg cggcaatcta cctccaggaa
     2281 ggtcagaaca acgacaagtt cttcacccac cccaaggatg ccagagcgct ggcggcctac
     2341 ctcttcgtcc acaaccactt cttctacatg atggagctgc tcacggccct gctcctgctg
     2401 ctgctgtcgc tgtgcgagtc ccccgctgtc cccgtgctca agctgcacac ttacgtccac
     2461 gccacgctgg aactctttgc cctcatggtg gtggtatttg aactctgcat gaaattgcgg
     2521 tggctgggct tccacacgtt cgtccggcac aaacgtacca tggtcaagac gtccgtcctc
     2581 gtggtgcagt tcatcgaggc cattgtggtg ctggttcggc agacgtccca cgtgcgggtg
     2641 acccgggcac tacgctgcat tttcctggtg gactgtcgct actgtggcgg tgtacggcgc
     2701 aacctgcggc agatcttcca gtctctccca cccttcatgg acatcctcct gttgctgctc
     2761 ttctttatga tcatctttgc catcctcggt ttctacttat tctccacaaa tccttccgac
     2821 ccctacttca gcaccctgga gaacagcatc gtcaacctgt tcgttctcct gaccacagcc
     2881 aactttccag atgtcatgat gccctcctac tcccggaacc cctggtcctg cgtcttcttc
     2941 attgtatacc tctccattga gctgtacttc atcatgaacc tgctcctggc cgtggtgttc
     3001 gacaccttca acgacattga aaagcacaag ttcaagtctt tgctgctgca caaacggacc
     3061 gccatccagc atgcctacgg cctgcttgcc agccaacgga ggccggctgg catctcctac
     3121 aggcagttcg aaggcttaat gcgcttctac aagccccgga tgagtgcaag ggaacgcttc
     3181 ctgactttca aggccttgaa ccagagcaac acgcctctgc tcagcctgaa ggacttctat
     3241 gatatttacg aagtcgctgc tctgcagtgg aaggcaaaga gaaacagaca gcattggttt
     3301 gatgagctcc cccggacagc cttcctcatc ttcaaaggga ttaacatcct tgtgaattcc
     3361 aaggccttcc agtatttcat gtacttggtg gtggctgtca acggtgtctg gatcctggtg
     3421 gagacattca tgttgaaagg tgggaatttc acctcaaagc atgtgccctg gagttacctc
     3481 gtgtttctta ccatctatgg agttgaactg ttcatgaagg tggctggcct gggccctgtg
     3541 gagtacctgt cctctggatg gaacctgttc gatttctcgg tcacggcatt cgccttcctg
     3601 ggactgctcg cactgacgct caacatggaa cccttctatt tcattgtggt cctgcgtccc
     3661 cttcagctgc tgaggttatt taaactgaag aaacgctacc gcaacgtgtt ggacaccatg
     3721 tttgagctgc tgccgcggat ggccagcctt ggcctcacgc tgctcacctt ctactattcc
     3781 ttcgccatcg tgggcatgga gttcttcaac gggcggctga cccccaactg ctgcaacacc
     3841 agcacagtgg ccgacgccta ccggttcatc aaccacactg tgggcaataa gaccaaggta
     3901 gaggaaggct actactatct caacaacttt gacaacatcc tcaacagctt cgtgaccttg
     3961 tttgagctca ccgttgtcaa caattggtac atcatcatgg aaggcgtcac ctcgcagacg
     4021 tcccactgga gccgcctgta cttcatgacc ttttacatag tgaccatggt ggtgatgacc
     4081 attatcgtgg ccttcatcct ggaggccttc gtcttccgca tgaactacag ccgcaagagc
     4141 caggactcgg aagtggacag tggcatcgtc atcgagaagg aaatgtccaa ggaggagctt
     4201 atggccgtcc tggagcttta tcgtgaggag cgaggcacct cctctgacgt gacccggctg
     4261 ctggacaccc tctctcagat ggagaaatac cagcaaaatt ccatggtgtt tctgggacgg
     4321 cgatcgagaa ccaaaagtga cctgagtctg aagatgtacc aggaggagat ccaggagtgg
     4381 tacgaggagc atgcccggga acaggagcag cagaagctca ggggcagcgt gcccggccct
     4441 gcagcccagc agccccctgg cagtcgccag cgctcccaga ctgtcaccgc ggccgcggtg
     4501 agcaagggcg aggaggataa cttagccatc atcaaggagt tcatgcgctt caaggtgcac
     4561 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     4621 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     4681 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     4741 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     4801 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     4861 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     4921 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     4981 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     5041 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     5101 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     5161 gagggccgcc actccaccgg cggcatggac gagctgtaca agtagggatc ccgcgactct
     5221 agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
     5281 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
     5341 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
     5401 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
     5461 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag
     5521 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag
     5581 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc
     5641 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg
     5701 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg
     5761 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     5821 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     5881 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     5941 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6001 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6061 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6121 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6181 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6241 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6301 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc
     6361 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6421 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6481 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6541 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6601 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6661 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6721 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     6781 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6841 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6901 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     6961 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     7021 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     7081 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7141 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7201 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     7261 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7321 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     7381 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     7441 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     7501 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     7561 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     7621 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7681 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7741 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7801 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7861 gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc
     7921 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7981 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8041 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8101 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8161 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8221 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8281 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8341 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8401 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8461 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8521 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8581 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8641 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8701 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8761 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8821 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8881 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8941 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9001 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9061 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9121 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9181 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9241 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9301 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9361 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9421 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9481 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9541 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9601 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9661 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9721 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9781 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9841 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9901 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9961 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10021 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10081 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10141 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10201 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10261 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    10321 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10381 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
    10441 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    10501 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    10561 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    10621 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    10681 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    10741 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    10801 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    10861 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10921 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10981 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    11041 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    11101 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    11161 ttgactagcg gaggctagaa ggagagagat gggtgcgaga g
//
