LOCUS       sequence-267159-       10796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TPC2 with truncated N-terminus, tagged with mCherry on C terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10796)
  AUTHORS   Ruas M, Davis LC, Chen CC, Morgan AJ, Chuang KT, Walseth TF, Grimm 
            C, Garnham C, Powell T, Platt N, Platt FM, Biel M, Wahl-Schott C, 
            Parrington J, Galione A
  TITLE     Expression of Ca(2)(+)-permeable two-pore channels rescues NAADP 
            signalling in TPC-deficient cells.
  JOURNAL   EMBO J. 2015 Jul 2;34(13):1743-58. doi: 10.15252/embj.201490009. 
            Epub 2015 Apr 14.
  PUBMED    25872774
REFERENCE   2  (bases 1 to 10796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201490009"; journalName: "EMBO J"; date: "2015-07-2- 
            2"; volume: "34"; issue: "13"; pages: "1743-58"
COMMENT     Sequence Label: TPC2(Delta-N49)-mCherry
FEATURES             Location/Qualifiers
     source          1..10796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    160..275
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        333..636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        637..840
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     791..811
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     837..861
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3027..3734
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNLAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3169..3187)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3437..3456
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        3763..4262
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3798..3821)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4195..4214
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4283..4882
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4283..4302)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4779..4799
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4896..5484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4949..4969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5367..5378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5691..6324
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6453..6469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6453..6469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6466..6488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6477..6493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6501..6507,6508..6525,6526..6531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6546..6567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6684..6701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6855..7443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6935..6954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7614..8405,8406..8474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8237..8256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8475..8579)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8627..8761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8664..8683)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8718..8737
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8929..9562
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9609..9734
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10231..10464
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10649..10693
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
       61 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
      121 gatccattcg attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa aggggggatt
      181 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
      241 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
      301 gatccagttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
      361 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      421 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      481 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      541 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      601 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      661 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      721 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      781 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      841 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      901 agacaccgac tctactagag gatcgctagc gctaccggac tcagatctcg agctcaagct
      961 tcgaattcac catggatgct ggttcattgt ggctttaccg ctggtattac tcgaacgtct
     1021 gccaacgtgt gctgggcttc atcattttcc tgatcctgat cttggctttt gtggaggtcc
     1081 catcttcctt cacaaagact gcagatgtgc gctaccgttc ccagccctgg cagccgccct
     1141 gtggcctgac cgagacgatc gaggcgttct gcttgcttgc cttcctggtt gacctctctg
     1201 tgaagggcta cttggttggg caggcccagt tgcagcagaa cctgtggctg ctggcctact
     1261 ttatggtgct ggttgtgtct gtggtggact ggatcgtttc gctgagcctt gcttgtgagg
     1321 agcccctgcg gatgcgccgg ctgctccgcc ccttcttcct gctgcagaat tcctccatga
     1381 tgaagaagac cctgaagtgt atacggtggt cgctaccaga aatggccagt gtcgggctgc
     1441 tgctggccat ccacctgtgt ctcttcacca tcattgggat gctgctgttc accattggtg
     1501 agaaggatga agcacaggac caggagaggc tggcctactt ccggaacctt ccagaagcac
     1561 tgacctcact cctagtgctg ctgacgactt ccaacaaccc tgatgtgatg atccctgcgt
     1621 atactcagaa ccgggccttt gctctcttct tcatagtctt caccttgata ggaagcttgt
     1681 ttctgatgaa cctgctgaca gccatcatct ataatcagtt ccgtggctac ctaatgaaat
     1741 ctctacagac ctcactgttc cggcggcgac tgggggcccg tgcagcctac gaagtcctcg
     1801 cctccagggc cgggccagct ggaaccaccc ctgagttagt tggggtgaat cctgagacct
     1861 tcctgccagt gcttcagaaa acgcagctga acaaaaccca caaacaagcc attatgcaga
     1921 aggtgcagtc ctatgaaggc cgcccaatgc tggctgatga gttccagaaa ctcttcgatg
     1981 aggttgacaa aggtgtggcc aaagagcgcc cgctgaagcc ccaataccag tctccatttc
     2041 tgcagactgc ccagttcatc tttagccacc actacttcga ctacctgggg aacctcgtcg
     2101 ctctgggaaa cctcttgtct atttgtgtgt tcctggtgct ggactcagac ctgctgcctg
     2161 gggaacgtga tgactttgtc ctggggattc ttgactacat cttcatcttg tactacctgc
     2221 tggagctgct gtttaaggtg ttcgcgctgg gcctgccagg ctacctgtcc taccatagca
     2281 atgtgtttga tggcctcctc accatcatcc tgctggtttc ggagatttgc accctggctg
     2341 tataccgatt gccgcactca ggatggaagc cagagcagta tggcccactg tcgctctggg
     2401 acatgacacg actgatgaac acactgattg tgtttcgctt cctgcgcatc atccccaaca
     2461 taaagccaat ggctgaggta gccaacacca tcctgggcct aatccctaac ttgagggcat
     2521 ttggtgggat cctggtggtg gcatactatg tgtttgccat gatcgggatc aacctgttcc
     2581 gaggtgtcat cgtgcctcct ggaaacagca gcctggttcc cgacaacaac tcagctgtat
     2641 gtggaagctt tgagcagcta ggttactggc ccaacaactt tgacgacttt gctgctgctt
     2701 tgatcacact gtggaacgtg atggtggtga acaattggca ggtgatcctg gaagcctata
     2761 agcgctacgc aggcccgtgg tcaatggtgt attttgtgct atggtggctg gtgtcctctg
     2821 ttatctggat caacctgttt ctggctcttc ttctggagaa ctttctccac agatgggacc
     2881 ctcaaggtca taagcagctc cttgttggga ccaagcagat gtctgtggag ctcatgttca
     2941 gggatatcct agaagagccc aaggaggagg aactgatgga gaagctacac aaacacccac
     3001 acttgcatct gtgcagggcg gccgcggtga gcaagggcga ggaggataac ttagccatca
     3061 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     3121 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     3181 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     3241 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     3301 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     3361 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     3421 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     3481 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     3541 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     3601 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     3661 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     3721 agctgtacaa gtagggatcc cgcgactcta gataattcta ccgggtaggg gaggcgcttt
     3781 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     3841 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     3901 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     3961 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     4021 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     4081 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     4141 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     4201 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     4261 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4321 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4381 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4441 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4501 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4561 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4621 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4681 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     4741 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4801 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4861 gacccgcaag cccggtgcct gaccgcgtct ggaacaatca acctctggat tacaaaattt
     4921 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4981 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5041 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5101 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5161 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5221 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5281 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     5341 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5401 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5461 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     5521 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     5581 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     5641 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct
     5701 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     5761 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     5821 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     5881 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     5941 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     6001 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     6061 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     6121 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6181 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6241 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6301 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     6361 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag cgttttaccg
     6421 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6481 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6541 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6601 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6661 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6841 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6901 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6961 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7021 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7081 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7141 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7201 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7261 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7321 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7381 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7441 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7501 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7561 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7621 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7681 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7741 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7801 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7861 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7921 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7981 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8041 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8101 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8161 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8221 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8281 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8341 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8401 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8461 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8521 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8581 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
     8641 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8701 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     8761 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     8821 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     8881 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
     8941 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     9001 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     9061 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     9121 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     9181 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     9241 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     9301 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     9361 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     9421 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9481 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9541 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     9601 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     9661 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     9721 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     9781 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     9841 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     9901 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     9961 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    10021 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
    10081 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
    10141 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
    10201 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
    10261 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
    10321 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
    10381 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
    10441 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    10501 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
    10561 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    10621 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    10681 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
    10741 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttcta
//
