LOCUS       sequence-26716-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Andrews P, He YJ, Xiong Y
  TITLE     Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and 
            promotes cell growth by antagonizing p53 function.
  JOURNAL   Oncogene. 2006 Aug 3. 25(33):4534-48.
  PUBMED    16547496
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2006 Aug 3. 25(33):4534-48."
COMMENT     Sequence Label: pcDNA3-HA-Cul7
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             119..145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagcta tgtacccata cgatgttcct gactatgcgg gctatcccta
      121 tgacgtcccg gactatgcaa gcttggtacc gttaacatgg atcctgggag aactccgcta
      181 cagggaattc agggtgcccc tggggcccgg cttacatgcc tatcctgatg agctgatccg
      241 ccagcgcgtg ggccatgatg ggcatcctga gtaccagatc cgttggctca tcctgcggcg
      301 tggcgatgag ggggacgggg gctctggcca agtggactgc aaggctgagc acatcctgct
      361 gtggatgtcc aaggatgaga tctatgccaa ctgccacaag atgctgggcg aggatggcca
      421 ggtcatcggg ccctcccagg agtctgcagg ggaggttggg gccctggaca aatctgtgct
      481 ggaggagatg gaaaccgatg tgaagtccct cattcagaga gcccttcggc agctggagga
      541 gtgtgtgggc actatccctc ctgctcctct acttcacact gtccacgtgc tcagcgccta
      601 tgccagcatt gagcccctca ctggagtatt caaggancca ngggtcctgg acttg
//
