LOCUS       sequence-267183-        9613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ABE and ST2-com modified sgRNA scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Lyu P, Lu Z, Cho SI, Yadav M, Yoo KW, Atala A, Kim JS, Lu B
  TITLE     Adenine Base Editor Ribonucleoproteins Delivered by Lentivirus-Like 
            Particles Show High On-Target Base Editing and Undetectable RNA 
            Off-Target Activities.
  JOURNAL   CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1089/crispr.2020.0095.
  PUBMED    33616436
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2020"; journalName: "CRISPR J"; date: "2021-02"; 
            volume: "4"; issue: "1"; pages: "69-81"
COMMENT     Sequence Label: pspCas9-ABE-3'UTR-sgRNA-ST2-com vector
FEATURES             Location/Qualifiers
     source          1..9613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..291
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        292..495
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     446..466
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     537..556
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        537..555
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             605..625
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1814..1837,1838..1840,1841..5914)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1814..5914
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5915..5962
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(6272..6289)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6278..6485
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6497..6637
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6497..6626
                     /label=AAV2 ITR
     rep_origin      6712..7167
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6799..6818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7009..7030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7184..7203)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7303..7325
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7363..7381)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7449..7553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7554..7622,7623..8414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7772..7791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8585..9173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9074..9093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9235..9483
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9235..9255
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9406..9425
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gtacccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      121 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      181 attgacgtca atgacggtaa atggcccgcc tggcattgtg cccagtacat gaccttatgg
      241 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      301 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      361 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      421 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      481 tatataagca gagctggttt agtgaaccgt cagatccgct agagatccgc ggccgctaat
      541 acgactcact atagggagag ccgccaccat gaaacggaca gccgacggaa gcgagttcga
      601 gtcaccaaag aagaagcgga aagtctctga agtcgagttt agccacgagt attggatgag
      661 gcacgcactg accctggcaa agcgagcatg ggatgaaaga gaagtccccg tgggcgccgt
      721 gctggtgcac aacaatagag tgatcggaga gggatggaac aggccaatcg gccgccacga
      781 ccctaccgca cacgcagaga tcatggcact gaggcaggga ggcctggtca tgcagaatta
      841 ccgcctgatc gatgccaccc tgtatgtgac actggagcca tgcgtgatgt gcgcaggagc
      901 aatgatccac agcaggatcg gaagagtggt gttcggagca cgggacgcca agaccggcgc
      961 agcaggctcc ctgatggatg tgctgcacca ccccggcatg aaccaccggg tggagatcac
     1021 agagggaatc ctggcagacg agtgcgccgc cctgctgagc gatttcttta gaatgcggag
     1081 acaggagatc aaggcccaga agaaggcaca gagctccacc gactctggag gatctagcgg
     1141 aggatcctct ggaagcgaga caccaggcac aagcgagtcc gccacaccag agagctccgg
     1201 cggctcctcc ggaggatcct ctgaggtgga gttttcccac gagtactgga tgagacatgc
     1261 cctgaccctg gccaagaggg cacgcgatga gagggaggtg cctgtgggag ccgtgctggt
     1321 gctgaacaat agagtgatcg gcgagggctg gaacagagcc atcggcctgc acgacccaac
     1381 agcccatgcc gaaattatgg ccctgagaca gggcggcctg gtcatgcaga actacagact
     1441 gattgacgcc accctgtacg tgacattcga gccttgcgtg atgtgcgccg gcgccatgat
     1501 ccactctagg atcggccgcg tggtgtttgg cgtgaggaac gcaaaaaccg gcgccgcagg
     1561 ctccctgatg gacgtgctgc actaccccgg catgaatcac cgcgtcgaaa ttaccgaggg
     1621 aatcctggca gatgaatgtg ccgccctgct gtgctatttc tttcggatgc ctagacaggt
     1681 gttcaatgct cagaagaagg cccagagctc caccgactcc ggaggatcta gcggaggctc
     1741 ctctggctct gagacacctg gcacaagcga gagcgcaaca cctgaaagca gcgggggcag
     1801 cagcgggggg tcagacaaga agtacagcat cggcctggcc atcggcacca actctgtggg
     1861 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
     1921 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
     1981 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
     2041 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
     2101 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
     2161 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
     2221 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
     2281 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
     2341 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     2401 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
     2461 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
     2521 gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     2581 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
     2641 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     2701 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
     2761 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
     2821 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
     2881 cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa
     2941 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
     3001 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     3061 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
     3121 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
     3181 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
     3241 cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa
     3301 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
     3361 cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
     3421 cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg
     3481 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
     3541 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
     3601 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
     3661 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
     3721 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
     3781 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
     3841 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
     3901 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
     3961 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
     4021 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
     4081 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
     4141 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
     4201 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     4261 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
     4321 tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct
     4381 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
     4441 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
     4501 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
     4561 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
     4621 ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac
     4681 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
     4741 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
     4801 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
     4861 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
     4921 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
     4981 ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg
     5041 ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa
     5101 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
     5161 taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
     5221 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
     5281 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
     5341 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
     5401 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
     5461 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
     5521 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
     5581 gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc
     5641 caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca
     5701 ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
     5761 tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
     5821 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
     5881 gacacggatc gacctgtctc agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc
     5941 cggccaggca aaaaagaaaa agggatccta agctcgcttt cttgctgtcc aatttctatt
     6001 aaaggttcct ttgttcccta agtccaacta ctaaactggg ggatattatg aagggccttg
     6061 agcatctgga ttctgcctaa taaaaaacat ttattttcat tgctagctcg ctttcttgct
     6121 gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac tgggggatat
     6181 tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt tcattgcgaa
     6241 ttctagaaaa ttcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     6301 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6361 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6421 gggggtgggg tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct
     6481 ggggaggtac ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     6541 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     6601 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     6661 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     6721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6781 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6841 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6901 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     6961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7021 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     7081 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7141 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     7201 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     7261 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     7321 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     7381 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7441 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7501 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7561 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7621 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7681 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7741 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7801 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7861 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7921 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7981 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8041 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8101 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8161 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8221 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta
     8281 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8341 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8401 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8461 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8521 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8581 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8641 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8701 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8761 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8821 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8881 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8941 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9001 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9061 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9121 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9181 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtgagggc
     9241 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     9301 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     9361 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     9421 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     9481 accggtgtct tcctcgagga agacccgttt gagagctaga aatagcaagt tcaaataagg
     9541 ctagtccgtt atcaacttgg ctgaatgcct gcgagcatcc cacccaagtg gcaccgagtc
     9601 ggtgcttttt ttg
//
